NR5A2 promotes epithelial-to-mesenchymal transition in renal fibrosis by targeting MMP25 transcription DOI Creative Commons
Xiao Wang, Guang Chen,

Weimin Shan

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Июнь 7, 2024

Abstract Epithelial-to-mesenchymal transition (EMT) is crucial for the progression of renal tubulointerstitial fibrosis, typically leading to end-stage failure. The role Nuclear receptor subfamily 5 group A member 2 (NR5A2) in fibrosis not yet fully understood. This research assessed NR5A2 expression human and mouse models with unilateral ureteral obstruction (UUO). We further investigated NR5A2's function TGF-β1-driven tubular EMT. Our findings demonstrated a significant elevation profibrogenic agents like collagen-I(Col-Ⅰ), alpha-smooth muscle actin (α-SMA), fibronectin (FN) UUO-induced model. Suppressing blocking it ML180 diminished TGF-β1-induced E-cadherin, α-SMA, Col-Ⅰ , FN HK2 cells. Functionally, boosted MMP25 cells after TGF-β1 activation. Luciferase Chromatin Immunoprecipitation (ChIP) assays verified attachment distinct motif on promoter, initiating transcription. Crucially, silencing countered NR5A2-induced factors following These insights reveal that orchestrates TGF-β1-prompted EMT via MMP25, highlighting novel avenue curbing fibrosis.

Язык: Английский

Depleting profibrotic macrophages using bioactivated in vivo assembly peptides ameliorates kidney fibrosis DOI
Qing Ouyang, Chao Wang,

Tian Sang

и другие.

Cellular and Molecular Immunology, Год журнала: 2024, Номер 21(8), С. 826 - 841

Опубликована: Июнь 13, 2024

Язык: Английский

Процитировано

9
Spatiotemporal delivery of multiple components of rhubarb-astragalus formula for the sysnergistic treatment of renal fibrosis DOI Creative Commons
Qibin Zhang, Xiaofeng Ye, Lin Zhu

и другие.

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Окт. 2, 2024

Purpose Rhubarb ( Rheum palmatum L.) and astragalus (Radix astragali) find widespread used in clinical formulations for treating chronic kidney disease (CKD). Notably, the key active components, total rhubarb anthraquinone (TRA) saponin (TAS), exhibit superiority over terms of their clear composition, stability, quality control, small dosage, efficacy treatment. Additionally, polysaccharides (APS) significantly contribute to treatment renal fibrosis by modulating gut microbiota. However, due differences biopharmaceutical properties these achieving synergistic effects remains challenging. This study aims develop combined pellets (CPs) evaluate potential effect on unilateral ureteral obstruction (UUO)-induced fibrosis. Methods The CPs were obtained combining TRA/TAS-loaded SNEDDS APS-loaded pellets, prepared using fluidized bed coating process. underwent evaluation morphology, bulk density, hardness, flowing property. Moreover, vitro release payloads was evaluated with CHP Type I method. Furthermore, (UUO) model utilized investigate contribution microbiota modulation. Results ex-vivo demonstrated that developed not only improved dissolution TRA TAS but also delivered TRA/TAS APS spatiotemporally appropriate site along gastrointestinal tract. In an animal (UUO rats), oral administration ameliorated histological pathology, reduced collagen deposition, decreased levels inflammatory cytokines. restored disturbed induced UUO surgery protected intestinal barrier. Conclusion represent a promising strategy efficiently delivering components traditional Chinese medicine formulas, offering effective approach CKD.

Язык: Английский

Процитировано

6
Increased NPM1 inhibit ferroptosis and aggravate renal fibrosis via Nrf2 pathway in chronic kidney disease DOI Creative Commons

Wenjing Fu,

Mingyu Zhang, Yilin Meng

и другие.

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Год журнала: 2024, Номер 1871(1), С. 167551 - 167551

Опубликована: Окт. 20, 2024

Язык: Английский

Процитировано

5
Role of Histone Modifications in Kidney Fibrosis DOI Creative Commons

Shengyu Pan,

Tianhui Yuan,

Yuqi Xia

и другие.

Medicina, Год журнала: 2024, Номер 60(6), С. 888 - 888

Опубликована: Май 28, 2024

Chronic kidney disease (CKD) is characterized by persistent dysfunction, ultimately resulting in end-stage renal (ESRD). Renal fibrosis a crucial pathological feature of CKD and ESRD. However, there no effective treatment for this condition. Despite the complex molecular mechanisms involved fibrosis, increasing evidence highlights role histone modification its regulation. The reversibility modifications offers promising avenues therapeutic strategies to block or reverse fibrosis. Therefore, comprehensive understanding regulatory implications may provide novel insights into more safer approaches. This review recent advances particularly methylation acetylation. aim explore potential as targets treating

Язык: Английский

Процитировано

4
Editorial for “Evaluating the Potential of Quantitative Susceptibility Mapping for Detecting Iron Deposition of Renal Fibrosis in a Rabbit Model” DOI Open Access
Alexey Dimov, Martin R. Prince

Journal of Magnetic Resonance Imaging, Год журнала: 2025, Номер unknown

Опубликована: Фев. 17, 2025

Journal of Magnetic Resonance ImagingEarly View Editorial for "Evaluating the Potential Quantitative Susceptibility Mapping Detecting Iron Deposition Renal Fibrosis in a Rabbit Model" Alexey V. Dimov PhD, Corresponding Author PhD [email protected] orcid.org/0000-0003-0460-6635 Department Radiology, Weill Cornell Medicine, New York, USASearch more papers by this authorMartin R. Prince MD, Martin orcid.org/0000-0002-9883-0584 author First published: 17 February 2025 https://doi.org/10.1002/jmri.29729 Level Evidence: 5 Technical Efficacy: Stage 2 Read full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare text full-text accessPlease review our Terms and Conditions Use check box below share version article.I have read accept Wiley Online Library UseShareable LinkUse link article with your friends colleagues. Learn more.Copy URL Share linkShare onEmailFacebookxLinkedInRedditWechat No abstract is available article. References 1Kovesdy CP. Epidemiology chronic kidney disease: An update 2022. Kidney Int Suppl 2022; 12(1): 7-11. https://doi.org/10.1016/j.kisu.2021.11.003. 10.1016/j.kisu.2021.11.003 Web Science®Google Scholar 2Huang R, Fu P, Ma L. fibrosis: From mechanisms therapeutic medicines. Signal Transduct Target Ther 2023; 8:129. https://doi.org/10.1038/s41392-023-01379-7. 10.1038/s41392-023-01379-7 CASPubMedGoogle 3Klinkhammer BM, Boor P. Emerging diagnostic strategies. Mol Aspects Med 93:101206. https://doi.org/10.1016/j.mam.2023.101206. 10.1016/j.mam.2023.101206 4Morrell GR, Zhang JL, Lee VS. resonance imaging fibrotic kidney. J Am Soc Nephrol 2017; 28(9): 2564-2570. https://doi.org/10.1681/ASN.2016101089. 10.1681/ASN.2016101089 CASPubMedWeb 5Friedli I, Crowe LA, Berchtold L, et al. magnetic index renal fibrosis assessment: A comparison between diffusion-weighted T1 mapping histological validation. Sci Rep 2016; 6:30088. https://doi.org/10.1038/srep30088. 10.1038/srep30088 6Zhang JG, Xing ZY, Zha TT, Longitudinal assessment rabbit induced unilateral ureteral obstruction using two-dimensional susceptibility weighted imaging. Magn Reson Imaging 2018; 47(6): 1572-1577. https://doi.org/10.1002/jmri.25915. 10.1002/jmri.25915 PubMedWeb 7Zha T, Z, Pan Evaluating potential quantitative detecting iron deposition model. 2025. https://doi.org/10.1002/jmri.29722. 10.1002/jmri.29722 PubMedGoogle Early ViewOnline Version Record before inclusion an issue ReferencesRelatedInformation

Язык: Английский

Процитировано

0
Noninvasive grading of renal interstitial fibrosis and prediction of annual renal function loss in chronic kidney disease: the optimal solution of seven MR diffusion models DOI Creative Commons

Guanjie Yuan,

Zhouyan Liao,

Ping Liang

и другие.

Renal Failure, Год журнала: 2025, Номер 47(1)

Опубликована: Март 25, 2025

To explore the optimal choice of seven diffusion models (DWI, IVIM, DKI, CTRW, FROC, SEM, and sADC) to assess renal interstitial fibrosis (IF) annual function loss in chronic kidney disease (CKD). One hundred thirty-three CKD patients 30 controls underwent multi-b sequence scans. Patients were divided into training, testing, temporal external validation sets. Least absolute shrinkage selection operator regression logistic used select metrics for distinguishing mild from moderate-to-severe IF. The performances imaging, clinical, combined compared. A linear mixed-effects model calculated estimated glomerular filtration rate (eGFR) slope, multiple assessed association between 1-3-year eGFR slopes. sets had 75, 30, 28 patients, respectively. incorporating cortical fIVIM, MKDKI was superior clinical combining 24-hour urinary protein all (net reclassification index [NRI] > 0, p < 0.05). Decision curve analysis showed provided greater net benefit across most thresholds. Fifty-two, 35, 16 completed 1-, 2-, 3-year follow-ups. After adjusting covariates, fIVIM correlated with 1-year slope (β = 30.600, 0.001), αSEM 2- slopes 44.859, 0.002; β 95.631, 0.019). provides a useful comprehensive tool grading IF, as potential biomarkers progression.

Язык: Английский

Процитировано

0
Bioinformatics analysis and experimental validation of potential targets and pathways in chronic kidney disease associated with renal fibrosis DOI Creative Commons

Cui Huimin,

Zhao Yuxin,

Peng Wang

и другие.

Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)

Опубликована: Апрель 2, 2025

Chronic kidney disease (CKD) has emerged as a major health problem worldwide. Previous studies have shown that specific miRNA expression profiles of patients with CKD are significantly changed. In this study, we aim to elucidate the role miRNAs potential biomarkers in progression by integrating bioinformatics analysis experimental validation, thereby providing medical evidence for prevention and treatment CKD. Bioinformatics was used identify targets pathways CKD-associated renal fibrosis through randomly obtaining microarray data related Gene Expression Omnibus (GEO) database according inclusion exclusion criteria, conducting pathway enrichment constructing protein-protein interaction (PPI) networks miRNA-mRNA network Cytoscape 3.8.0. vitro experiments were employed verify mechanism miR-223-3p human tubular epithelial cells (HK2) Quantitative real-time PCR assays, Western blot, Immunofluorescence Double luciferase reporter gene experiment. Multi-group one-way variance (ANOVA) Dunnett-t test uesd analyze results SPSS24.0. 10 up-regulated 11 down-regulated screened out. Phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) first analysis. MiR-223-3p (logFC=-2.047, p = 0.002) one four hub miRNAs. Furthermore, observed reduction α-smooth muscle actin (α-SMA) (p 0.001) Collagen type I alpha 1 (Col1-a1) 0.023) levels upon overexpression, which aligned our predictions. This downregulation attributed inhibition nuclear factor kappa-B (NF-κB) translocation subsequent decrease secretion inflammatory cytokines, such interleukin-6 (IL-6) 0.005). Conversely, when CHUK further overexpressed, inhibitory effect on epithelial-mesenchymal transition (EMT) attenuated, confirming between CHUK. Our findings provide compelling acts suppressor EMT specifically targeting modulating PI3K/Akt pathway, holds great promise novel therapeutic target treatment. Additionally, study offers avenue development future interventions aimed at halting or reversing

Язык: Английский

Процитировано

0
Diagnostic Approaches in Hypertension and Chronic Kidney Disease Renal Markers in Evaluation and Follow-Up of Hypertension and Classical and Modern Imaging Techniques in Evaluation of BP Disorders DOI
Michel Burnier,

William Rodriguez,

Menno Pruijm

и другие.

Updates in hypertension and cardiovascular protection, Год журнала: 2025, Номер unknown, С. 273 - 301

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Perturbed gut microbiota and serum metabolites are associated with progressive renal fibrosis DOI Creative Commons

Run-Xi Wang,

Hong-Bing Zhou, Jin Gao

и другие.

Frontiers in Medicine, Год журнала: 2025, Номер 12

Опубликована: Апрель 28, 2025

The intricate pathogenesis of renal fibrosis necessitates identifying biomarkers at various stages to facilitate targeted therapeutic interventions, which would enhance patient survival rates and significantly improve prognosis. We investigated the changes in gut microbiota serum metabolites during early, middle, late rats using 16S rDNA sequencing UPLC-QTOF/MS-based metabolomics. identified 5, 21, 14 potential microbial markers 19, 23, 31 metabolic MOD1, MOD2, MOD4 groups, respectively. Bifidobacterium was as a shared marker between MOD1 MOD2 groups; Prevotellaceae_NK3B31_group Bacteroides were groups. pathways arachidonic acid metabolism retinol found play significant role modulation 1, 2, 4 weeks. Notably, 8,9-EET 5(S)-HPETE within these emerged critical determinants influencing fibrosis. Our findings demonstrated that severity is associated with dysbiosis alterations metabolites.

Язык: Английский

Процитировано

0
Cellular crosstalk in fibrosis: insights into macrophage and fibroblast dynamics DOI Creative Commons

Zachary S.C.S. Froom,

Neal I. Callaghan, Locke Davenport Huyer

и другие.

Journal of Biological Chemistry, Год журнала: 2025, Номер unknown, С. 110203 - 110203

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0