Plastic
products
and
nutrients
are
widely
used
in
aquaculture
facilities,
resulting
copresence
of
nanoplastics
(NPs)
released
from
plastics
microcystins
(MCs)
algae.
The
potential
effects
NPs-MCs
coexposure
on
aquatic
require
investigation.
This
study
investigated
the
toxic
polystyrene
(PS)
NPs
MC-LR
gut-liver
axis
silver
carp
Hypophthalmichthys
molitrix,
a
representative
commercial
fish,
explored
intestinal
microorganism
structure
liver
metabolic
function
using
traditional
toxicology
multi-omics
association
analysis.
results
showed
that
PS-NPs
significantly
shortened
villi
length,
higher
concentration
PS-NPs,
more
obvious
shortening.
high
concentrations
increased
hepatocyte
space
caused
loss
gill
filaments.
diversity
richness
fish
gut
microbes
after
exposure,
this
trend
was
amplified
MC-LR.
In
coexposure,
contributed
to
alteration
metabolism,
which
affected
enrichment
pathway
glycerophospholipid
metabolism
folic
acid
biosynthesis,
there
correlation
between
differential
metabolites
bacteria.
These
suggested
mechanism
may
be
pathological
changes
tissues,
microbiota
disturbance,
imbalance.
findings
not
only
improve
understanding
environmental
risks
combined
with
other
pollutants,
but
also
provide
biomarkers
carp.
Metabolites,
Год журнала:
2024,
Номер
14(12), С. 682 - 682
Опубликована: Дек. 5, 2024
Background:
In
recent
years,
titanium
dioxide
(TiO2)
nanoparticles
(NPs)
have
been
widely
used
in
various
industries
due
to
their
favorable
chemical
properties,
and
contamination
of
the
environment
has
attracted
much
attention,
especially
aquatic
animals.
Methods:
Therefore,
we
assessed
impact
TiO2
NPs
(5
mg/L)
on
marine
bivalve,
pearl
oyster
(Pinctada
fucata
martensii),
gill
metabolism.
Pearl
oysters
were
exposed
seawater
containing
5
mg/L
for
14
days,
followed
by
7
days
recovery
untreated
seawater.
Gill
tissues
hepatopancreatic
sampled
0,
14,
21
experiment
named
C0,
E14,
R7,
respectively.
Results:
Metabolomic
analysis
identified
102
significantly
different
metabolites
(SDMs)
gills
tissue
following
exposure
(C0
vs.
E14).
Compared
with
group
E14
had
76
SDMs
(such
as
acetylcholine,
itaconic
acid,
citric
taurine)
higher
concentrations
26
(including
L-arginine
isobutyryl-L-carnitine)
lower
concentrations.
KEGG
(Kyoto
Encyclopedia
Genes
Genomes)
showed
that
these
enriched
28
pathways,
including
glycine,
serine,
threonine
metabolism,
neuroactive
ligand–receptor
interaction,
taurine
hypotaurine
addition,
116
R7
oysters.
74
6-phosphogluconic
isocitric
acid)
42
uracil,
glycerophosphocholine,
N-Acetyl-D-glucosamine)
The
between
25
pentose
phosphate
pathway,
glutathione
citrate
cycle
(TCA
cycle).
energy
metabolism-associated
enzymes
revealed
reduced
Ca2+/Mg2+-ATPase,
Na+/K+-ATPase,
Total-ATPase
activities.
Conclusions:
These
findings
suggested
may
inhibit
metabolism
function
hepatopancreas
Meanwhile,
affect
normal
functioning
immune
osmoregulatory
functions
even
lead
oxidative
stress
neurotoxicity.
this
study
provide
a
reference
analyzing
bioadaptation
bivalves
potential
negative
effects
bivalves.
Plastic
products
and
nutrients
are
widely
used
in
aquaculture
facilities,
resulting
copresence
of
nanoplastics
(NPs)
released
from
plastics
microcystins
(MCs)
algae.
The
potential
effects
NPs-MCs
coexposure
on
aquatic
require
investigation.
This
study
investigated
the
toxic
polystyrene
(PS)
NPs
MC-LR
gut-liver
axis
silver
carp
Hypophthalmichthys
molitrix,
a
representative
commercial
fish,
explored
intestinal
microorganism
structure
liver
metabolic
function
using
traditional
toxicology
multi-omics
association
analysis.
results
showed
that
PS-NPs
significantly
shortened
villi
length,
higher
concentration
PS-NPs,
more
obvious
shortening.
high
concentrations
increased
hepatocyte
space
caused
loss
gill
filaments.
diversity
richness
fish
gut
microbes
after
exposure,
this
trend
was
amplified
MC-LR.
In
coexposure,
contributed
to
alteration
metabolism,
which
affected
enrichment
pathway
glycerophospholipid
metabolism
folic
acid
biosynthesis,
there
correlation
between
differential
metabolites
bacteria.
These
suggested
mechanism
may
be
pathological
changes
tissues,
microbiota
disturbance,
imbalance.
findings
not
only
improve
understanding
environmental
risks
combined
with
other
pollutants,
but
also
provide
biomarkers
carp.
