Strategies for promoting neurovascularization in bone regeneration

Military Medical Research, Год журнала: 2025, Номер 12(1)

Опубликована: Март 3, 2025

Abstract Bone tissue relies on the intricate interplay between blood vessels and nerve fibers, both are essential for many physiological pathological processes of skeletal system. Blood provide necessary oxygen nutrients to bone tissues, remove metabolic waste. Concomitantly, fibers precede during growth, promote vascularization, influence cells by secreting neurotransmitters stimulate osteogenesis. Despite critical roles components, current biomaterials generally focus enhancing intraosseous vessel repair, while often neglecting contribution nerves. Understanding distribution main functions in is crucial developing effective engineering. This review first explores anatomy highlighting their vital embryonic development, metabolism, repair. It covers innovative regeneration strategies directed at accelerating intrabony neurovascular system over past 10 years. The issues covered included material properties (stiffness, surface topography, pore structures, conductivity, piezoelectricity) acellular biological factors [neurotrophins, peptides, ribonucleic acids (RNAs), inorganic ions, exosomes]. Major challenges encountered neurovascularized materials clinical translation have also been highlighted. Furthermore, discusses future research directions potential developments aimed producing repair that more accurately mimic natural healing tissue. will serve as a valuable reference researchers clinicians novel into practice. By bridging gap experimental practical application, these advancements transform treatment defects significantly improve quality life patients with bone-related conditions.

Язык: Английский

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A Versatile Chitosan‐Based Hydrogel Accelerates Infected Wound Healing via Bacterial Elimination, Antioxidation, Immunoregulation, and Angiogenesis

Advanced Healthcare Materials, Год журнала: 2024, Номер 13(19)

Опубликована: Фев. 26, 2024

Abstract Drug‐resistant bacterial infection of cutaneous wounds causes great harm to the human body. These infections are characterized by a microenvironment with recalcitrant infections, persistent oxidative stress, imbalance immune regulation, and suboptimal angiogenesis. Treatment strategies available date incapable handling healing dynamics infected wounds. A Schiff base borate ester cross‐linked hydrogel, based on phenylboronic acid‐grafted chitosan (CS‐PBA), dibenzaldehyde‐grafted poly(ethylene glycol), tannic acid (TA), is fabricated in present study. Customized acid‐modified zinc oxide nanoparticles (ZnO) embedded hydrogel prior gelation. The CPP@ZnO‐P‐TA effectively eliminates methicillin‐resistant Staphylococcus aureus (MRSA) due pH‐responsive release Zn 2+ TA. Killing achieved via membrane damage, adenosine triphosphate reduction, leakage intracellular components, hydrolysis o ‐nitrophenyl‐β‐ d ‐galactopyranoside. capable scavenging reactive oxygen nitrogen species, alleviating stimulating M2 polarization macrophages. released TA also induce neovascularization PI3K/Akt pathway. improves tissue regeneration vivo inflammatory responses, angiogenesis, facilitating collagen deposition. findings suggest that this versatile possesses therapeutic potential for treatment MRSA‐infected

Язык: Английский

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Biomaterials science and surface engineering strategies for dental peri-implantitis management

Military Medical Research, Год журнала: 2024, Номер 11(1)

Опубликована: Май 13, 2024

Abstract Peri-implantitis is a bacterial infection that causes soft tissue inflammatory lesions and alveolar bone resorption, ultimately resulting in implant failure. Dental implants for clinical use barely have antibacterial properties, colonization biofilm formation on the dental are major of peri-implantitis. Treatment strategies such as mechanical debridement antibiotic therapy been used to remove plaque. However, it particularly important prevent occurrence peri-implantitis rather than treatment. Therefore, current research spot has focused improving properties implants, construction specific micro-nano surface texture, introduction diverse functional coatings, or application materials with intrinsic properties. The aforementioned surfaces can be incorporated bioactive molecules, metallic nanoparticles, other components further enhance osteogenic accelerate healing process. In this review, we summarize recent developments biomaterial science modification applied inhibit facilitate bone-implant integration. Furthermore, summarized obstacles existing process laboratory reach clinic products, propose corresponding directions future perspectives, so provide insights into rational design aim balance efficacy, biological safety, property.

Язык: Английский

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Bioinspired, Bioactive, and Bactericidal: Anodized Nanotextured Dental Implants

Advanced Functional Materials, Год журнала: 2024, Номер 34(30)

Опубликована: Фев. 22, 2024

Abstract Bioinspired titanium implant nanotexturing achieved via advanced techniques such as lithography, hydrothermal and laser patterning enables modulation of cell functions toward enhanced bioactivity antibacterial efficacy. However, costly, multi‐step methods limit clinical translation benchtop modification. For the first time, single‐step, cost‐effective, translatable electrochemical anodization is reported to fabricate bioinspired nanopillar‐like textures on micro‐rough titanium. In‐depth surface characterization confirms formation novel nanostructures, namely nanoscale Spinules , Daggers Papillae Spikes Flames exhibiting varied roughness wettability. Next, in separate experiments, primary human osteoblasts polymicrobial salivary biofilm are cultured substrates. Nanotextured surfaces show high protein adhesion maintain proliferation, adhesion, spreading osteoblasts. Interestingly, all nanotextures exhibit superior antibiofilm abilities compared control surfaces. Bioactive nano‐texturing single‐step has potential for next generation orthopedic dental

Язык: Английский

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“Photo-Thermo-Electric” Dental Implant for Anti-Infection and Enhanced Osteoimmunomodulation

ACS Nano, Год журнала: 2024, Номер 18(36), С. 24968 - 24983

Опубликована: Авг. 28, 2024

The dental implant market has experienced explosive growth, owing to the widespread acceptance of implants as core oral rehabilitation. Clinically, achieving simultaneous anti-infective effects and rapid osseointegration is a crucial but challenging task for implants. demand with long-term broad-spectrum antibacterial immune-osteogenic properties growing. Existing methods are limited by lack safety, efficiency, short-lasting ability, inadequate consideration immunomodulatory on osteogenesis. Herein, ZnO/black TiO

Язык: Английский

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Regulation of immune microenvironments by polyetheretherketone surface topography for improving osseointegration

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Март 11, 2025

Optimizing the immune microenvironment is essential for successful implant osseointegration. In this study, four different nano/microstructures were fabricated on polyetheretherketone (PEEK) substrates by varying agitation speed during sulfonation to influence osteoimmunomodulation and integration. The results indicate that with minimal dimensions (SP450) inhibit actin polymerization reducing calcium influx through PIEZO1, activating anti-inflammatory M2 macrophage phenotype. Among tested specimens, SP450 exhibited lowest expression levels of tumor necrosis factor-α interleukin-1β while releasing highest factors, including interleukin-4 interleukin-10. This optimized environment promotes osteogenesis MC3T3-E1 pre-osteoblasts enhances osseointegration PEEK implants. Transcriptomic analysis validation experiment further revealed inhibits osteoclastic differentiation down-regulating transforming growth factor-β2 suppressing NF-κB signaling pathway. These findings suggest manipulating surface topography implants an effective strategy enhancing promising clinical applications.

Язык: Английский

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Lateral Spacing of TiO2 Nanotube Coatings Modulates In Vivo Early New Bone Formation

Biomimetics, Год журнала: 2025, Номер 10(2), С. 81 - 81

Опубликована: Янв. 28, 2025

Due to the bio-inert nature of titanium (Ti) and subsequent accompanying chronic inflammatory response, an implant’s stability function can be significantly affected, which is why various surface modifications have been employed, including deposition oxide (TiO2) nanotubes (TNTs) onto native through anodic oxidation method. While influence nanotube diameter on cell behaviour osteogenesis very well documented, information regarding effects lateral spacing in vivo new bone formation process insufficient hard find. Considering this, present study’s aim was evaluate mechanical properties osteogenic ability two types TNTs-based pins with different spacing, e.g., 25 nm 92 (spTNTs). The TNT-coated implants were characterised from a morphological point view (tube diameter, tube length) using scanning electron microscopy (SEM). In addition, chemical composition evaluated X-ray photoelectron spectroscopy, while roughness topography atomic force (AFM). Finally, implants’ hardness elastic modulus investigated nanoindentation measurements. histologically (haematoxylin eosin—HE staining) at 6 30 days post-implantation rat model. Mechanical characterisation revealed that morphologies presented similar strength, but, terms roughness, spTNTs exhibited higher average roughness. microscopic examination 1 month (57.21 ± 34.93) capable promoting early tissue greater extent than TNTs-coated (24.37 6.5), difference thickness newly formed ~32.84 µm, thus highlighting importance this parameter when designing future dental/orthopaedic implants.

Язык: Английский

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Amino-Functionalized Mesoporous Silica Film as a Spatiotemporally Matched Degradable Nanotopography to Enhance Early Bioactivity and Osteogenesis on Titania Nanotube Surfaces

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Март 31, 2025

Nanotopographic fabrication has been proven to enhance the osteoinductivity of titanium implant surfaces; however, it is difficult for static nanostructures regulate multiple osteoblast behaviors. Herein, we proposed a novel strategy further modifying nanostructured surfaces using degradable nanotopography that was beneficial specific cellular processes and spatiotemporally matched. In this work, titania nanotube (TNT) array, known its strong capability promote osteogenic differentiation, employed as substrate. An oil-water biphase system containing 3-aminopropyl triethoxysilane (APTES) tetraethyl orthosilicate (TEOS) utilized achieve in situ deposition amino-functionalized mesoporous silica films on TNT surface (TNT@AHMS). The numerous mesopores (∼4 nm) amino groups AHMS significantly improved protein adsorption attachment rat bone marrow mesenchymal stem cells (rBMSCs). By culturing an AHMS-conditioned medium, effects enhancing early cell behavior were observed initially attributed potential synergism topography silicon element release (∼18 ppm). Impressively, effect maintained even when reseeded normal culture substrates. After 24 h, degraded completely, degradation products facilitated subsequent re-exposed TNT, which accounted robust osteogenesis both vitro vivo. This study demonstrated can serve (like buffer layer) accelerate adhesion matched manner, resulting enhanced bioactivity well-designed underlying without influencing physicochemical properties.

Язык: Английский

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Surface Modification of TiO2 Nanotubes via Pre-Loaded Hydroxyapatite Towards Enhanced Bioactivity

Materials Today Communications, Год журнала: 2024, Номер 39, С. 109216 - 109216

Опубликована: Май 13, 2024

Anodic TiO2 nanotubes (NTs) are widely established in biomedical applications, as the sub-100 nm morphology significantly impacts their biological activity. In this study, we examine use and surface functionalization of nanotube layers on titanium substrates to facilitate formation hydroxyapatite, a crucial ability for implant applications. NT grown by electrochemical anodization focus is as-formed anatase NTs with 100- 15-nm-diameters, latter amorphous available double-wall (DT) or single-wall (ST) structures. Surface modification achieved through an alternate immersion method (AIM) simple CaCl2 immersion. The former deposits hydroxyapatite (HA) coatings on/in layers, while forms thin Ca-surface-modified layer surface. Both methods effectively induce HA 100-nm-diameter after five days simulated body fluid (SBF). chemical composition deciding factor, that already contain phosphates (from anodizing electrolyte) also lead via Ca-functionalization (CaCl2 immersion). Whereas, smaller diameter NTs, nanotopography DT ST key nucleation AIM approach, but not calcium-containing solution. This promising approach accelerates growth nanomaterials initiating apatite and, thus, has significant implications increasing bioactivity

Язык: Английский

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Innovation of TiO2-x Nanomaterials in the Biomedical Field: Synthesis, Properties, and Application Prospects

Chemical Engineering Journal, Год журнала: 2024, Номер 491, С. 151773 - 151773

Опубликована: Май 7, 2024

Язык: Английский

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