Accelerating the Discovery of Abiotic Vesicles with AI-Guided Automated Experimentation DOI
M. C. Ekosso, Hao Liu,

Avery Glagovich

и другие.

Langmuir, Год журнала: 2024, Номер 41(1), С. 858 - 867

Опубликована: Дек. 30, 2024

The first protocells are speculated to have arisen from the self-assembly of simple abiotic carboxylic acids, alcohols, and other amphiphiles into vesicles. To study complex process vesicle formation, we combined laboratory automation with AI-guided experimentation accelerate discovery specific compositions underlying principles governing formation. Using a low-cost commercial liquid handling robot, automated experimental procedures, enabling high-throughput testing various reaction conditions for mixtures seven (7) amphiphiles. Multitemplate multiscale template matching (MMTM) was used automate confocal microscopy image analysis, us quantify formation without tedious manual counting. results were create Gaussian surrogate model, then active learning iteratively direct experiments reduce model uncertainty. Mixtures containing primarily trimethyl decylammonium decylsulfate in equal amounts formed vesicles at submillimolar critical concentrations, more than 20% glycerol monodecanoate prevented forming even high total amphiphile concentrations.

Язык: Английский

Autonomous mobile robots for exploratory synthetic chemistry DOI Creative Commons

Tianwei Dai,

Sriram Vijayakrishnan, Filip Szczypiński

и другие.

Nature, Год журнала: 2024, Номер 635(8040), С. 890 - 897

Опубликована: Ноя. 6, 2024

Abstract Autonomous laboratories can accelerate discoveries in chemical synthesis, but this requires automated measurements coupled with reliable decision-making 1,2 . Most autonomous involve bespoke equipment 3–6 , and reaction outcomes are often assessed using a single, hard-wired characterization technique 7 Any algorithms 8 must then operate narrow range of data 9,10 By contrast, manual experiments tend to draw on wider instruments characterize products, decisions rarely taken based one measurement alone. Here we show that synthesis laboratory be integrated into an by mobile robots 11–13 make human-like way. Our modular workflow combines robots, platform, liquid chromatography–mass spectrometer benchtop nuclear magnetic resonance spectrometer. This allows share existing human researchers without monopolizing it or requiring extensive redesign. A heuristic decision-maker processes the orthogonal data, selecting successful reactions take forward automatically checking reproducibility any screening hits. We exemplify approach three areas structural diversification chemistry, supramolecular host–guest chemistry photochemical synthesis. strategy is particularly suited exploratory yield multiple potential as for assemblies, where also extend method function assay evaluating binding properties.

Язык: Английский

Процитировано

16

Autonomous Size-Targeting for Block Copolymer Nanoparticles DOI

Oliver Weismantel,

Lakshani J. Weerarathna, Tanja Junkers

и другие.

ACS Applied Polymer Materials, Год журнала: 2025, Номер unknown

Опубликована: Янв. 2, 2025

Using the combination of flow synthesis with online dynamic light scattering (DLS) analysis for particle size characterization and fully autonomous computer control allows reproducible targetable nanoparticles from block copolymer (BCP) solutions by rapid mixing water in a defined micromixer environment. Bayesian optimization (BO), nanoparticle sizes become programmable preselectable, wide range can be obtained per used BCP. Specifically, we show series polystyrene-b-poly(N,N-dimethyl acrylamide) polystyrene-b-poly(poly(ethylene glycol) methyl ether acrylate) BCPs how particles spanning 130 to 280 nm systematically targeted, between 100 1000 being at least principle also achievable. Further, Pareto fronts individual parameters overall rate, volume fraction, polymer concentration are established routines presented. This BO approach highlights efficacy platforms achieving precise over self-assembly processes, offering an optimal production window development polymeric nanostructures diverse fields, such as drug delivery materials science.

Язык: Английский

Процитировано

1

Do-it-yourself instrument integration into an existing mammalian cell line development automation platform DOI Creative Commons

Jie Ding,

Kee W. Tan, Xiaoyue Chen

и другие.

SLAS TECHNOLOGY, Год журнала: 2025, Номер 31, С. 100246 - 100246

Опубликована: Янв. 9, 2025

Язык: Английский

Процитировано

0

Solvent Screening for Separation Processes Using Machine Learning and High-Throughput Technologies DOI Creative Commons

Justin P. Edaugal,

Difan Zhang, Dupeng Liu

и другие.

Chem & Bio Engineering, Год журнала: 2025, Номер 2(4), С. 210 - 228

Опубликована: Март 5, 2025

As the chemical industry shifts toward sustainable practices, there is a growing initiative to replace conventional fossil-derived solvents with environmentally friendly alternatives such as ionic liquids (ILs) and deep eutectic (DESs). Artificial intelligence (AI) plays key role in discovery design of novel development green processes. This review explores latest advancements AI-assisted solvent screening specific focus on machine learning (ML) models for physicochemical property prediction separation process design. Additionally, this paper highlights recent progress automated high-throughput (HT) platforms screening. Finally, discusses challenges prospects ML-driven HT strategies optimization. To end, provides insights advance future

Язык: Английский

Процитировано

0

Rapid synthesis of metastable materials for electrocatalysis DOI
Qiao Chen,

Zichao Xi,

Ziyuan Xu

и другие.

Chemical Society Reviews, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Metastable materials are considered promising electrocatalysts for clean energy conversions by virtue of their structural flexibility and tunable electronic properties. However, the exploration synthesis metastable via traditional equilibrium methods face challenges because requirements high precise control. In this regard, rapid method (RSM), with efficiency ultra-fast heating/cooling rates, enables production under non-equilibrium conditions. relationship between RSM properties remains largely unexplored. review, we systematically examine unique benefits various techniques mechanisms governing formation materials. Based on these insights, establish a framework, linking electrocatalytic performance Finally, outline future directions emerging field highlight importance high-throughput approaches autonomous screening optimal electrocatalysts. This review aims to provide an in-depth understanding electrocatalysts, opening up new avenues both fundamental research practical applications in electrocatalysis.

Язык: Английский

Процитировано

0

Science acceleration and accessibility with self-driving labs DOI Creative Commons
Richard B. Canty, Jeffrey A. Bennett, Keith A. Brown

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Апрель 24, 2025

Язык: Английский

Процитировано

0

Computer-Aided Retrosynthesis for Greener and Optimal Total Synthesis of a Helicase-Primase Inhibitor Active Pharmaceutical Ingredient DOI Creative Commons

Rodolfo I. Teixeira,

Michael Andresini, Renzo Luisi

и другие.

JACS Au, Год журнала: 2024, Номер 4(11), С. 4263 - 4272

Опубликована: Окт. 2, 2024

This study leverages and upgrades the capabilities of computer-aided retrosynthesis (CAR) in systematic development greener more efficient total synthetic routes for active pharmaceutical ingredient (API) IM-204, a helicase-primase inhibitor that demonstrated enhanced efficacy against Herpes simplex virus (HSV) infections. Using various CAR tools, several were uncovered, evaluated, experimentally validated, with goal to maximize selectivity yield minimize environmental impact. The tools revealed options under different constraints, which can overperform patented route used as reference. selected CAR-based significant improvement from 8% (patented route) 26%, along moderate overall green performance. It was also shown human-in-the-loop approach be synergistically combined drive further improvements deliver alternatives. strategy metrics by substituting solvents merging two steps into one. These changes led IM-204 synthesis 8 35%. Additionally, performance score, based on GreenMotion metrics, improved 0 18, cost building blocks reduced 550-fold. work demonstrates potential drug development, highlighting its capacity streamline processes, reduce footprint, lower production costs, thereby advancing field toward sustainable practices.

Язык: Английский

Процитировано

1

Modeling Enzyme Kinetics: Current Challenges and Future Perspectives for Biocatalysis DOI
Jürgen Pleiss

Biochemistry, Год журнала: 2024, Номер unknown

Опубликована: Сен. 26, 2024

Biocatalysis is becoming a data science. High-throughput experimentation generates rapidly increasing stream of biocatalytic data, which the raw material for mechanistic and novel data-driven modeling approaches predictive design improved biocatalysts bioprocesses. The holistic molecular understanding enzymatic reaction systems will enable us to identify overcome kinetic bottlenecks shift thermodynamics reaction. full characterization community effort; therefore, published methods results should be findable, accessible, interoperable, reusable (FAIR), achieved by developing standardized exchange formats, complete reproducible documentation experimentation, collaborative platforms sustainable software analyzing repositories publishing together with data. FAIRification biocatalysis prerequisite highly automated laboratory infrastructures that improve reproducibility scientific reduce time costs required develop synthesis routes.

Язык: Английский

Процитировано

0

Accelerating the Discovery of Abiotic Vesicles with AI-Guided Automated Experimentation DOI
M. C. Ekosso, Hao Liu,

Avery Glagovich

и другие.

Langmuir, Год журнала: 2024, Номер 41(1), С. 858 - 867

Опубликована: Дек. 30, 2024

The first protocells are speculated to have arisen from the self-assembly of simple abiotic carboxylic acids, alcohols, and other amphiphiles into vesicles. To study complex process vesicle formation, we combined laboratory automation with AI-guided experimentation accelerate discovery specific compositions underlying principles governing formation. Using a low-cost commercial liquid handling robot, automated experimental procedures, enabling high-throughput testing various reaction conditions for mixtures seven (7) amphiphiles. Multitemplate multiscale template matching (MMTM) was used automate confocal microscopy image analysis, us quantify formation without tedious manual counting. results were create Gaussian surrogate model, then active learning iteratively direct experiments reduce model uncertainty. Mixtures containing primarily trimethyl decylammonium decylsulfate in equal amounts formed vesicles at submillimolar critical concentrations, more than 20% glycerol monodecanoate prevented forming even high total amphiphile concentrations.

Язык: Английский

Процитировано

0