Thinking small to win big? A critical review on the potential application of extracellular vesicles for biomarker discovery and new therapeutic approaches in pancreatic cancer DOI Creative Commons

Mahrou Vahabi,

Annalisa Comandatore,

Chiara Centra

и другие.

Seminars in Cancer Biology, Год журнала: 2023, Номер 97, С. 50 - 67

Опубликована: Ноя. 11, 2023

Pancreatic ductal adenocarcinoma (PDAC) is an extremely deadly form of cancer, with limited progress in 5-year survival rates despite significant research efforts. The main challenges treating PDAC include difficulties early detection, and resistance to current therapeutic approaches due aggressive molecular microenvironment features. These emphasize the importance identifying clinically validated biomarkers for detection clinical management. Extracellular vesicles (EVs), particularly exosomes, have emerged as crucial mediators intercellular communication by transporting cargo. Recent has unveiled their role initiation, metastasis, chemoresistance PDAC. Consequently, utilizing EVs liquid biopsies holds promise identification prognosis, monitoring drug efficacy. However, numerous limitations, including isolation characterization homogeneous populations, well absence standardized protocols, can affect reliability studies involving biomarkers, underscoring necessity a prudent approach. also garnered considerable attention promising delivery system novel therapy tumors. loading biomolecules or chemical drugs into exosomes subsequent target cells effectively impede tumor progression. Nevertheless, there are obstacles that must be overcome ensure accuracy efficacy therapies relying on treatment In this review, we examine both recent advancements remaining obstacles, exploring potential biomarker discovery development vehicles.

Язык: Английский

Cancer organoid co-culture model system: Novel approach to guide precision medicine DOI Creative Commons
Jin Yuan, Xiaoyang Li, Shengji Yu

и другие.

Frontiers in Immunology, Год журнала: 2023, Номер 13

Опубликована: Янв. 12, 2023

Three-dimensional cancer organoids derived from self-organizing stems are ex vivo miniatures of tumors that faithfully recapitulate their structure, distinctive features, and genetic signatures. As novel tools, current have been well established rapidly applied in drug testing, genome editing, transplantation, with the ultimate aim entering clinical practice for guiding personalized therapy. However, given lack a tumor microenvironment, including immune cells fibrous cells, is major limitation this emerging methodology, co-culture models inspire high hope further application technology research. Co-culture or fibroblasts available to investigate molecular interactions, chimeric antigen receptor-engineered lymphocytes treatment. In light recent progress organoid models, it only possible recognize advantages drawbacks model exploit its full potential. review, we summarize advances how they could be improved future benefit research, especially precision medicine.

Язык: Английский

Процитировано

58
Engineered matrices reveal stiffness-mediated chemoresistance in patient-derived pancreatic cancer organoids DOI
Bauer L. LeSavage, D.H. Zhang, Carla Huerta-López

и другие.

Nature Materials, Год журнала: 2024, Номер 23(8), С. 1138 - 1149

Опубликована: Июль 4, 2024

Язык: Английский

Процитировано

31
Extracellular signals regulate the biogenesis of extracellular vesicles DOI Creative Commons
Yong Jin,

Lele Ma,

Wanying Zhang

и другие.

Biological Research, Год журнала: 2022, Номер 55(1)

Опубликована: Ноя. 26, 2022

Abstract Extracellular vesicles (EVs) are naturally released membrane that act as carriers of proteins and RNAs for intercellular communication. With various biomolecules specific ligands, EV has represented a novel form information transfer, which possesses extremely outstanding efficiency specificity compared to the classical signal transduction. In addition, extended concept transduction aspect by working collection extracellular information. Therefore, functions EVs have been extensively characterized exhibit an exciting prospect clinical applications. However, biogenesis and, in particular, regulation this process signals, essential conduct further studies support optimal utility, remain unclear. Here, we review current understanding EVs, focus on signals discuss their therapeutic value.

Язык: Английский

Процитировано

60
The role of biomechanical stress in extracellular vesicle formation, composition and activity DOI Creative Commons
Will Thompson,

Eleftherios T. Papoutsakis

Biotechnology Advances, Год журнала: 2023, Номер 66, С. 108158 - 108158

Опубликована: Апрель 26, 2023

Язык: Английский

Процитировано

37
PDAC, the Influencer Cancer: Cross-Talk with Tumor Microenvironment and Connected Potential Therapy Strategies DOI Open Access

Leonardo Mercanti,

Maria Sindaco,

Mariangela Mazzone

и другие.

Cancers, Год журнала: 2023, Номер 15(11), С. 2923 - 2923

Опубликована: Май 26, 2023

Pancreatic ductal adenocarcinoma (PDAC) is among the leading causes of death by cancer in world. What makes this pathological condition particularly lethal a combination clinical and molecular heterogeneity, lack early diagnostic indexes, underwhelming results from current therapeutic protocols. A major cause PDAC chemoresistance seems to lie ability cells spread out fill pancreatic parenchyma, exchanging nutrients, substrates, even genetic material with surrounding tumor microenvironment (TME). Several components can be found TME ultrastructure, including collagen fibers, cancer-associated fibroblasts, macrophages, neutrophils, mast cells, lymphocytes. Cross-talk between latter being converted into cancer-favoring phenotypes; behavior could compared an influencer guiding followers supporting his activity. Moreover, potential target for some newest strategies; these include use pegvorhyaluronidase-α CAR-T lymphocytes against HER2, FAP, CEA, MLSN, PSCA, CD133. Other experimental therapy options are currently studied, aiming interfere KRAS pathway, DNA-repairing proteins, apoptosis resistance cells. Hopefully new approaches will grant better outcomes future patients.

Язык: Английский

Процитировано

29
Strategy of targeting the tumor microenvironment via inhibition of fibroblast/fibrosis remodeling new era to cancer chemo-immunotherapy resistance DOI
Mahboubeh Tajaldini,

Amirhoushang Poorkhani,

Taghi Amiriani

и другие.

European Journal of Pharmacology, Год журнала: 2023, Номер 957, С. 175991 - 175991

Опубликована: Авг. 23, 2023

Язык: Английский

Процитировано

24
Patient-derived organoids in precision cancer medicine DOI Creative Commons
Le Tong, Weiyingqi Cui, Boya Zhang

и другие.

Med, Год журнала: 2024, Номер unknown

Опубликована: Сен. 1, 2024

Язык: Английский

Процитировано

13
Formation of pre-metastatic niches induced by tumor extracellular vesicles in lung metastasis DOI Creative Commons
Yuping Liu,

Dengxuan Mao,

Hong Wang

и другие.

Pharmacological Research, Год журнала: 2023, Номер 188, С. 106669 - 106669

Опубликована: Янв. 18, 2023

There are a number of malignant tumors that metastasize into the lung as one their most common sites dissemination. The successful infiltration tumor cells distant organs is result cooperation between and host cells. When have not yet reached organs, in situ secrete extracellular vesicles (EVs) carrying important biological information. In recent years, scholars found cells-derived EVs act bridge orthotopic secondary metastases by promoting formation pre-metastatic niche (PMN), which plays key role awakening dormant circulating cell colonization. This review provides an overview multiple routes mechanisms underlying PMN induced summaries study findings underline potential intervention PMN, both target or carrier for drug design. this review, highlighted well applications to metastasis diagnosis treatment.

Язык: Английский

Процитировано

23
Engineered 3D ex vivo models to recapitulate the complex stromal and immune interactions within the tumor microenvironment DOI Creative Commons
Kalpana Ravi, Twinkle Jina Minette Manoharan,

Kuei-Chun Wang

и другие.

Biomaterials, Год журнала: 2023, Номер 305, С. 122428 - 122428

Опубликована: Дек. 19, 2023

Язык: Английский

Процитировано

15
A pancreatic cancer organoid-in-matrix platform shows distinct sensitivities to T cell killing DOI Creative Commons

Anton Lahusen,

Jierui Cai,

Reinhold Schirmbeck

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Апрель 23, 2024

Abstract Poor treatment responses of pancreatic ductal adenocarcinoma (PDAC) are in large part due to tumor heterogeneity and an immunosuppressive desmoplastic stroma that impacts interactions with cells the microenvironment (TME). Thus, there is a pressing need for models probe contributions cellular noncellular crosstalk. Organoids promising model systems potential generate plethora data including phenotypic, transcriptomic genomic characterization but still require improvements culture conditions mimicking TME. Here, we describe INTERaction Organoid-in-MatriX ("InterOMaX") system, presents 3D co-culture-based platform investigating matrix-dependent We its uncover new molecular mechanisms T cell murine KPC ( LSL- Kras G12D /+27 /Trp53 tm1Tyj/J /p48 Cre/ + ) PDAC as well patient-derived organoids (PDOs). For this, customizable matrix homogenously sized organoid-in-matrix positioning cancer were designed based on standardized agarose microwell chip array system established co-culture inclusion stromal cells. detection orthogonal analysis human populations distinct sensitivity killing corroborated vivo . By enabling both identification validation gene candidates resistance, this sets stage better mechanistic understanding cell-intrinsic resistance phenotypes PDAC.

Язык: Английский

Процитировано

6