Langmuir,
Год журнала:
2024,
Номер
41(1), С. 945 - 954
Опубликована: Дек. 30, 2024
The
remarkable
flexibility
in
structural
tunability
and
designability
of
poly(ionic
liquids)
(PILs)
has
garnered
significant
attention.
Integration
PILs
with
membranes,
novel
properties,
functionalities
is
anticipated
for
applications
the
fields
membrane
separation.
Here,
we
develop
a
facile
method
to
prepare
PIL-functionalized
membranes
one-step
process
by
combining
selective
swelling-induced
pore
generation
ionic
liquid
functionalization.
block
copolymer
poly(2-dimethylaminoethyl
methacrylate)-block-polystyrene
(PDMAEMA-b-PS,
abbreviated
as
SDMA)
films
immersed
mixture
ethanol
bromopropane.
In
addition
formation
nanoporous
structures,
an
interfacial
quaternization
reaction
between
PDMAEMA
blocks
bromopropane
occurs
generate
poly(methacrylatoethyl
propyl
dimethylammonium
bromide),
resulting
PIL-Br-functionalized
(SIL-Br)
during
swelling
process.
It
noteworthy
that
acting
reactant
also
promotes
swelling.
water
permeability
SIL-Br
several
times
higher
than
SDMA
membrane,
which
attributed
increased
size
significantly
hydrophilicity
membrane.
addition,
anion
exchange
l-proline
(l-Pro)
readily
forms
SIL-Pro-functionalized
(SIL-Pro),
exhibit
exceptional
electrical
neutrality.
Antifouling
tests
demonstrate
both
SIL-Pro
have
excellent
resistance
proteins
compared
non-PIL-functionalization
implying
their
great
potential
antifouling
treatment.
Molecules,
Год журнала:
2024,
Номер
29(10), С. 2381 - 2381
Опубликована: Май 18, 2024
The
development
of
immobilized
enzymes
with
high
activity
and
stability
is
critical.
Metal–organic
frameworks
(MOFs)
have
attracted
much
academic
industrial
interest
in
the
field
enzyme
immobilization
due
to
their
unique
properties.
In
this
study,
amino-functionalized
ionic
liquid
(NIL)-modified
metal–organic
framework
(UiO-66-NH2)
was
prepared
immobilize
Candida
rugosa
lipase
(CRL),
using
dialdehyde
starch
(DAS)
as
cross-linker.
results
Fourier
transform
infrared
(FT-IR)
spectra,
X-ray
powder
diffraction
(XRD),
scanning
electronic
microscopy
(SEM)
confirmed
that
NIL
successfully
grafted
UiO-66-NH2.
CRL
on
NIL-modified
UiO-66-NH2
(UiO-66-NH2-NIL-DAS@CRL)
exhibited
satisfactory
recovery
(79.33%),
stability,
reusability,
excellent
organic
solvent
tolerance.
research
indicated
liquid-modified
had
practical
potential
for
application
immobilization.
International Journal of Biological Macromolecules,
Год журнала:
2024,
Номер
278, С. 135046 - 135046
Опубликована: Авг. 24, 2024
This
study
harnesses
glutamate
decarboxylase
(GAD)
from
Yarrowia
lipolytica
to
improve
the
biosynthesis
of
γ-aminobutyric
acid
(GABA),
focusing
on
boosting
enzyme's
catalytic
efficiency
and
stability
by
immobilizing
it
nanofibrous
membranes.
Through
recombinant
DNA
techniques,
two
GAD
genes,
YlGAD1
YlGAD2,
were
cloned
then
expressed
in
Escherichia
coli.
Compared
their
soluble
forms,
immobilized
enzymes
exhibited
significant
improvements
thermal
pH
increased
resistance
chemical
denaturants.
The
immobilization
notably
enhanced
substrate
affinity,
as
evidenced
reduced
K
