Synthetic Biology Tools to Engineer Microbial Communities for Biotechnology

Trends in biotechnology, Год журнала: 2018, Номер 37(2), С. 181 - 197

Опубликована: Ноя. 26, 2018

Microbial consortia have been used in biotechnology processes, including fermentation, waste treatment, and agriculture, for millennia. Today, synthetic biologists are increasingly engineering microbial diverse applications, the bioproduction of medicines, biofuels, biomaterials from inexpensive carbon sources. An improved understanding natural ecosystems, development new tools to construct program their behaviors, will vastly expand functions that can be performed by communities interacting microorganisms. Here, we review recent advancements biology approaches engineer consortia, discuss ongoing emerging efforts apply various biotechnological suggest future applications.

Язык: Английский

---

The Chemistry of Plant–Microbe Interactions in the Rhizosphere and the Potential for Metabolomics to Reveal Signaling Related to Defense Priming and Induced Systemic Resistance

Frontiers in Plant Science, Год журнала: 2018, Номер 9

Опубликована: Фев. 8, 2018

Plant roots communicate with microbes in a sophisticated manner through chemical communication within the rhizosphere, thereby leading to biofilm formation of beneficial and, case plant growth-promoting rhizomicrobes / -bacteria (PGPR), resulting priming defense or induced resistance host. The knowledge plant-plant and plant-microbe interactions have been greatly extended over recent years; however, is far from being well understood. Furthermore, linkage between below- above-ground physiological processes adds complexity. In metabolomics studies, main aim profile annotate all exo- endo-metabolites biological system that drive participate processes. Recent advances this field has enabled researchers analyze hundreds compounds one sample short time period. Here, viewpoint, we review rhizosphere subsequent 'signalomics', emphasize contributions mass spectrometric-based metabolomic approaches can bring study plant-beneficial – events.

Язык: Английский

---

Emerging and evolving concepts in gene essentiality

Nature Reviews Genetics, Год журнала: 2017, Номер 19(1), С. 34 - 49

Опубликована: Окт. 16, 2017

Язык: Английский

---

Modular co-culture engineering, a new approach for metabolic engineering

Metabolic Engineering, Год журнала: 2016, Номер 37, С. 114 - 121

Опубликована: Май 28, 2016

Язык: Английский

---

Massively parallel screening of synthetic microbial communities

Proceedings of the National Academy of Sciences, Год журнала: 2019, Номер 116(26), С. 12804 - 12809

Опубликована: Июнь 11, 2019

Microbial communities have numerous potential applications in biotechnology, agriculture, and medicine. Nevertheless, the limited accuracy with which we can predict interspecies interactions environmental dependencies hinders efforts to rationally engineer beneficial consortia. Empirical screening is a complementary approach wherein synthetic are combinatorially constructed assayed high throughput. However, assembling many combinations of microbes logistically complex difficult achieve on timescale commensurate microbial growth. Here, introduce kChip, droplets-based platform that performs rapid, massively parallel, bottom-up construction communities. We first show kChip enables phenotypic characterization across conditions. Next, screen ∼100,000 multispecies comprising up 19 soil isolates, identified sets promote growth model plant symbiont

Язык: Английский

---

Next-generation biocontainment systems for engineered organisms

Nature Chemical Biology, Год журнала: 2018, Номер 14(6), С. 530 - 537

Опубликована: Май 16, 2018

Язык: Английский

---

Revisiting Plant–Microbe Interactions and Microbial Consortia Application for Enhancing Sustainable Agriculture: A Review

Frontiers in Microbiology, Год журнала: 2020, Номер 11

Опубликована: Дек. 21, 2020

The present scenario of agricultural sector is dependent hugely on the use chemical-based fertilizers and pesticides that impact nutritional quality, health status, productivity crops. Moreover, continuous release these chemical inputs causes toxic compounds such as metals to accumulate in soil move plants with prolonged exposure, which ultimately human health. Hence, it becomes necessary bring out alternatives pesticides/fertilizers for improvement outputs. rhizosphere plant an important niche abundant microorganisms residing it. They possess properties growth promotion, disease suppression, removal compounds, assimilating nutrients plants. Utilizing beneficial microbes crop presents efficient way modulate yield by maintaining healthy status quality through bioformulations. To understand microbial formulation compositions, essential processes going well their concrete identification better utilization diversity growth–promoting bacteria arbuscular mycorrhizal fungi. this background, review article highlights microbiome aboveground belowground, importance inoculants various species, subsequent interactive mechanisms sustainable agriculture.

Язык: Английский

---

Toward low-cost biological and hybrid biological/catalytic conversion of cellulosic biomass to fuels

Energy & Environmental Science, Год журнала: 2022, Номер 15(3), С. 938 - 990

Опубликована: Янв. 1, 2022

Hybrid processes, featuring biological conversion of lignocellulose to small molecules followed by chemo-catalytic larger suitable for difficult-to-electrify transport modes, are a promising route biomass-derived fuels in demand climate stabilization.

Язык: Английский

---

Biotechnological potential and applications of microbial consortia

Biotechnology Advances, Год журнала: 2019, Номер 40, С. 107500 - 107500

Опубликована: Дек. 18, 2019

Язык: Английский

---

A heterogeneous microbial consortium producing short-chain fatty acids from lignocellulose

Science, Год журнала: 2020, Номер 369(6507)

Опубликована: Авг. 28, 2020

Microbial consortia are a promising alternative to monocultures of genetically modified microorganisms for complex biotransformations. We developed versatile consortium-based strategy the direct conversion lignocellulose short-chain fatty acids, which included funneling lignocellulosic carbohydrates lactate as central intermediate in engineered food chains. A spatial niche enabled situ cellulolytic enzyme production by an aerobic fungus next facultative anaerobic lactic acid bacteria and product-forming anaerobes. Clostridium tyrobutyricum, Veillonella criceti, or Megasphaera elsdenii were integrated into platform produce 196 kilograms butyric per metric ton beechwood. The demonstrates benefits mixed cultures, such their modularity ability convert substrates valuable biochemicals.

Язык: Английский

---