Coinfection of SARS-CoV-2 and influenza A (H3N2) detected in bronchoalveolar lavage fluid of a patient with long COVID using metagenomic next−generation sequencing: a case report

Frontiers in Cellular and Infection Microbiology, Год журнала: 2023, Номер 13

Опубликована: Сен. 1, 2023

The growing number of long COVID cases has drawn clinical attention to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which been spreading worldwide since winter 2019. Its symptoms are not limited fatigue and shortness breath but also affect daily life. We report the use metagenomic next-generation sequencing (mNGS) detect coinfection with SARS-CoV-2 influenza A virus in a patient COVID. was admitted fever, expectoration, fatigue, breath. PCR test negative due possible clearance SARS-Cov-2 upper tract patients Other routine microbiological tests were negative, making diagnosis difficult. Bronchoalveolar lavage fluid (BALF) samples tested using mNGS. diagnosed treated promptly, recovered quickly, continued taking azvudine after discharge; his condition stable. This study illustrates that mNGS may be valuable for timely their mixed infections.

Язык: Английский

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The effect of COVID-19 on the frequency of Pneumocystis jirovecii pneumonia: a monocentric, retrospective, and observational study

BMC Infectious Diseases, Год журнала: 2023, Номер 23(1)

Опубликована: Окт. 17, 2023

Pneumocystis jirovecii pneumonia (PCP) and SARS-CoV2 share some similarities in their effects on the respiratory system, clinical presentation, management. The COVID-19 pandemic required rapid action to curb transmission mitigate its lethiferous impact. Non-pharmaceutical interventions (NPIs) were globally adopted. We hypothesized that these measures reduced acquisition of P. both hospital community settings.We conducted a retrospective observational study 2950 specimens from patients with suspected pulmonary infection, analyzed at Laboratory Parasitology Unit Policlinico Tor Vergata Rome, Italy, January 2014 December 2022.We show significant reduction frequency PCP era compared previous period. Among four sequence types identified, genotype 1 was most prevalent (37%). observed non-significant trend decreasing cases increasing 3 over period.The nationwide implementation NPIs against may have changed microbiological landscape exposure, thereby exposure consequently reducing incidence PCP.

Язык: Английский

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The effect of COVID-19 on the frequency of Pneumocystis jirovecii pneumonia: a monocentric, retrospective, and observational study

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Июнь 6, 2023

Abstract Background Pneumocystis jirovecii pneumonia (PCP) and SARS-CoV2 share some similarities in their effects on the respiratory system, clinical presentation, management. The COVID-19 pandemic required rapid action to curb transmission mitigate its lethiferous impact. Non-pharmaceutical interventions (NPIs) were globally adopted. We hypothesized that these measures reduced acquisition of P. both hospital community settings. Methods conducted a retrospective observational study 2950 specimens from patients with suspected pulmonary infection, analyzed at Laboratory Parasitology Unit Policlinico Tor Vergata Rome, Italy, January 2014 December 2022. Results show significant reduction frequency PCP era, compared previous period. Among four sequence types identified, genotype 1 was most prevalent (37%). observed non-significant trend decreasing cases increasing 3 over Conclusions nationwide implementation NPIs against may have changed microbiological landscape exposure, thereby exposure consequently reducing incidence PCP.

Язык: Английский

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In-vitro Interactions between Fluconazole and Diphenyl Diselenide against Various Candida Species

Current Bioactive Compounds, Год журнала: 2024, Номер 20(10)

Опубликована: Фев. 2, 2024

Background: In the immunocompromised population, Candida species are most aetiologic agents causing severe nosocomial fungal infections. species, irrespective of being commensals in human microbiome, fourth prevalent source potentially fatal yeast Monotherapy is frequently employed to treat invasive infections, but sometimes, patients do not favor monotherapy treatment regime. It may be because reduced susceptibility pathogen toward traditional antimycotic drugs. Antimycotic drug combination therapy could a better choice such specific circumstances. our study, we evaluated interactions fluconazole with diphenyl diselenide. Methods: The susceptibilities for and diselenide were determined by broth microdilution assay, in-vitro studied using disc diffusion assay chequerboard assay. nature was assessed calculating fractional inhibitory concentration index (FICI). also analyzed response surface approach. Results: minimum concentrations (MICs) as against 4 μg/ml-512 μg/ml 1 μg/ml-32 μg/ml, respectively. FICI values varied from 0.375 2. Conclusion: Our finding demonstrated that there no antagonism interaction between species. Thus, this innovative should explored future.

Язык: Английский

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Targeted next-generation sequencing of pathogens reveals the profile of secondary infections in COVID-19 patients

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Март 21, 2024

Abstract PURPOSE: To use targeted next-generation sequencing (tNGS) of pathogens for analysing the etiological distribution secondary infections in patients with severe and critical novel coronavirus pneumonia (COVID-19), to obtain microbial epidemiological data on COVID-19, provide a reference early empirical antibiotic treatment such patients. METHODS: Patients COVID-19 hospitalised at First Affiliated Hospital Shandong Medical University between 1 December 2022 30 June 2023 were included study. The characteristics these analysed using tNGS. RESULTS: A total 95 study, whom 87.37% had one or more underlying diseases. Forty-eight detected, most common being HSV-4, Candida albicans, Klebsiella pneumoniae, Enterococcus faecium, HSV-1, Staphylococcus aureus, Aspergillus fumigatus, Acinetobacter baumannii, HSV-5, Stenotrophomonas maltophilia, Pneumocystis jirovecii detected 14.29% cases. majority (76.84%) mixed infections, viral-bacterial-fungal (28.42%). CONCLUSION: Most are mixed, high rates viral fungal infections. In clinical settings, monitoring reactivation by Herpesviridae viruses is crucial; additionally, have significantly higher rate P. infection. tNGS testing bronchoalveolar lavage fluid can help determine aetiology assist choosing appropriate antibiotics.

Язык: Английский

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Post-COVID-19 Fungal Infections: A Case Series

Journal of Advanced Lung Health, Год журнала: 2024, Номер 4(3), С. 181 - 185

Опубликована: Июль 16, 2024

Severe acute respiratory syndrome coronavirus 2-like other viral infections cause temporary immunosuppressive effects. This COVID-19 infection-induced suppression of cellular immunity can predispose to like fungal. Furthermore, high-dose corticosteroids used in management trigger or accelerate fungal infections. case series presents the clinicomicrobiological profile a few such admitted cases, as it is very important for all clinicians and clinical microbiologists keep new yet recently not-so-uncommon entities mind while evaluating patient.

Язык: Английский

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Respiratory Tract Pathogen Profiles of COVID-19 Pneumonia Patients and the Mortality Prediction

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Июль 22, 2024

Background COVID-19 pneumonia is easily complicated with other respiratory pathogenic attacks, increasing the risk of death. Exploring pathogen profiles patients-related facilitated clinical management and decisions to pursue better prognosis. Methods This study systematically compared sputum culture results death events 170 non-COVID-19 197 patients. Statistical analysis was carried out find profile difference between two populations. The model constructed for infected COVID-19. Results It found that co-infection bacteria fungi increased mortality isolation rate Acinetobacter baumannii in patients significantly higher than often showed multi-drug resistant phenotypes. a incidence intensive care unit admission, ventilator-assisted ventilation fungal infection. serum levels interleukin-1, interleukin-6, interleukin-8, TNF, lymphocytes, neutrophils white blood cells decreased. A prediction based on machine learning methods, achieving accuracy 90.0%. main factors affecting survival co-infected pathogens were admission unit, days hospital stay, ventilator-aided treatment, carbapenems administration, lymphocyte, aspartate aminotransferase level, infection, Candida Conclusions provided necessary indicators timely precise intervention when they by pathogens. COVID-19-related secondary infection microorganisms different isolated from

Язык: Английский

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A Cluster of Diutina catenulata Funguria in Patients with Coronavirus Disease 2019 (COVID-19) Hospitalized in a Tertiary Reference Hospital from Rio de Janeiro, Brazil

Current Microbiology, Год журнала: 2024, Номер 81(10)

Опубликована: Сен. 3, 2024

Язык: Английский

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Invasive Aspergillosis with Cavernous Sinus Thrombosis Following High-Dose Corticosteroid Therapy: A Challenging Case of Rhino-Orbital-Cerebral Mycosis

Journal of Fungi, Год журнала: 2024, Номер 10(11), С. 788 - 788

Опубликована: Ноя. 13, 2024

Invasive aspergillosis is a rare but severe fungal infection primarily affecting immunocompromised individuals. The Coronavirus Disease-2019 (COVID-19) pandemic has introduced new complexities in managing due to the widespread use of corticosteroids for treating COVID-19-related respiratory distress, which can increase susceptibility infections. Here, we present challenging case progressive cerebral complicated by cavernous sinus thrombosis (CST) 67-year-old male with history COVID-19. patient, initially misdiagnosed temporal arteritis, received pulse corticosteroid therapy twice before presenting persistent left-sided headaches and vision loss. Cranial imaging revealed findings consistent sinusitis, Tolosa–Hunt syndrome, orbital pseudotumor, progressed despite initial antifungal therapy. Subsequent magnetic resonance indicated an invasive mass extending into left other intracranial structures, raising suspicion aspergillosis. A transsphenoidal biopsy confirmed Aspergillus infection, leading voriconazole Despite aggressive treatment, follow-up significant progression, extension right frontal region sinus. patient then developed visual impairment eye was diagnosed CST secondary sinusitis. Management included combination systemic antifungals antibiotics; however, declined surgical intervention. This underscores diagnostic challenges rapid progression associated post-COVID-19 patients treated corticosteroids. report highlights need heightened clinical prompt, targeted interventions similar cases improve outcomes. Further research required understand optimal management

Язык: Английский

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Study of Outcomes of Mucormycosis in COVID-19 Patients at a Tertiary Care Hospital in Central India: A Retrospective Study

Cureus, Год журнала: 2024, Номер unknown

Опубликована: Дек. 15, 2024

Background The COVID-19 pandemic has posed unprecedented challenges to the global healthcare system. Among various complications, mucormycosis, a fungal infection caused by Mucorales order, emerged as significant threat, particularly in immunocompromised individuals. This study aims evaluate outcomes of mucormycosis patients treated at tertiary care hospital Central India. Method retrospective reviewed medical records 72 diagnosed with following between April 2021 and July hospital. Data on demographics, clinical features, comorbidities, treatment received (surgery, antifungal medications), (mortality, response treatment) were collected analyzed. Results mean age was 55.42±12.31 years, male predominance (n=44; 61.11%). Facial pain (n=61; 84.72%) headache (n=58; 80.55%) most common features. duration symptoms 14.31±5.4 days. Steroids used majority (n=50; 69%). comorbidity diabetes mellitus (n=42; 58.33%). Out patients, 40 (55.6%) survived 32 (44.4%) died. Surgical debridement performed 45 (62.5%) patients. All (100%) amphotericin B, an medication. older lack early surgical intervention factors associated higher mortality COVID-19. Conclusion reinforces critical role diagnosis, immediate systemic therapy, stringent management prompt improving for

Язык: Английский

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