Pathogenicity of Commensal Gut Biofilm In Prefrail Aging

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 4, 2024

Abstract Achieving healthy aging is a global challenge requiring deeper understanding of mechanisms, spanning from robustness to frailty. Although age-related alteration the gut microbiome (microb-aging) well studied, there lack research investigating microbiota during early stages prefrailty and its behavior within context natural community lifestyle, in interaction with host tissue. Therefore, we used fecal samples human cohort INSPIRE-T, characterize polymicrobial biofilms. Intestinal tissues feces an mouse were also used. Food supplement was tested for potential geroprotective effects on Findings pointed taxonomic physical alterations, increased instability, virulence regards intestinal epithelium, biofilms prefrail individuals, compared their age-matched robust counterparts. Multiparametric analyses further linked biofilm characteristics clinical traits, suggesting that culture could be proxy tool evaluating or unhealthy status. Exposure grape pomace prebiotic partially reversed prefail phenotype. In aged mice prefrailty, but not mice, structure altered caused colon damage when transplanted into antibiotic-depleted mice. These findings suggest causal link between microb-aging inflammation, advocating microbiota-targeted therapies reverse phenotype thereby promote healthspan.

Язык: Английский

Microbiota of the distal ileum under antibiotic prophylaxis: Does fecal exposure during intracorporeal intestinal urinary diversion increase gastrointestinal complications?

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 12, 2024

Background No consensus exists for perioperative antibiotic prophylaxis intracorporeal intestinal urinary diversion owing to the lack of evidence regarding microbiota distal ileum. This study aimed identify cultivable in ileum under and its impact on postoperative gastrointestinal complications. Methods A retrospective analysis was conducted 131 patients with bladder cancer who underwent at our institution between 2019 2024. Fecal samples were collected directly from ileum, ascitic fluid abdominal drains day three. Results The ileal feces generally sparse 114 (87.0%), whereas 17 (13.0%) exhibited substantial growth. rate positive cultures proportional number colonies cultured feces. complications (intra-abdominal infections / ileus) significantly higher than those no growth either (36.9% vs. 8.5%, P = 0.002) or ascites (64.9% 11.7%, < 0.001). predominantly colonized by Streptococcus (46.4%), Enterococcus (23.8%), Enterobacter (22.6%), Klebsiella (11.9%), Candida (11.4%). All four (3%) developed intra-abdominal had both fecal cultures. In multivariate analysis, identified as independent predictors Moreover, frailty a notable predictor culture. Conclusions Under prophylaxis, substantially suppressed did not increase infections. However, presence intraperitoneal bacteria fungi is strongly associated Frailty may be resistance persistence fungi.

Язык: Английский