Spectral flow cytometry for detecting DNA cargo in malaria parasite-derived extracellular vesicles

Journal of Biological Chemistry, Год журнала: 2025, Номер unknown, С. 108481 - 108481

Опубликована: Апрель 1, 2025

Cells across biological kingdoms release extracellular vesicles (EVs) as a means of communication with other cells, be their friends or foes. This is indeed true for the intracellular malaria parasite Plasmodium falciparum (Pf), which utilizes EVs to transport bioactive molecules various human host systems. Yet, study this mode in research currently constrained due limitations high-resolution tools and absence commercial antibodies. Here, we demonstrate power an advanced spectral flow cytometry approach robustly detect secreted EVs, isolated from Pf-infected red blood cells. By labeling both EV membrane lipids DNA cargo within (non-antibody staining approach), were able subpopulation parasitic-derived enriched DNA. Furthermore, could quantitatively measure DNA-carrying two distinct stages parasite: rings trophozoites. Our findings showcase potential monitor dynamic changes nucleic acid pathogenic EVs.

Язык: Английский

Special issue: emerging technologies for infectious disease research

Microbes and Infection, Год журнала: 2024, Номер 26(7), С. 105386 - 105386

Опубликована: Июль 9, 2024

Язык: Английский