Опубликована: Янв. 1, 2024
Язык: Английский
Frontiers in Cellular and Infection Microbiology, Год журнала: 2024, Номер 14
Опубликована: Июль 18, 2024
Enterococcus faecalis is one of the main microorganisms that infects root canals, ranking among most prevalent associated with endodontic treatment failure. Given its pervasive presence in persistent infections, successful elimination crucial for effective and retreatment. Furthermore, can form biofilms - defense structures microbes use to fight environmental threats. These confer resistance against host immune system attacks antibiotic interventions. Consequently, poses a significant challenge complete eradication disease. In response, numerous scholars have discovered promising outcomes addressing within canals undertaken endeavors explore more efficacious approaches combating these biofilms. This study provides comprehensive review strategies mechanisms removal
Язык: Английский
Процитировано
14
PLoS ONE, Год журнала: 2024, Номер 19(5), С. e0301292 - e0301292
Опубликована: Май 14, 2024
Enterococcus faecalis, a Gram-positive bacterium, poses significant clinical challenge owing to its intrinsic resistance broad spectrum of antibiotics, warranting urgent exploration innovative therapeutic strategies. This study investigated the viability phage therapy as an alternative intervention for antibiotic-resistant E. with specific emphasis on comprehensive genomic analysis bacteriophage SAM-E.f 12. The investigation involved whole-genome sequencing 12 using Illumina technology, resulting in robust dataset detailed characterization. Bioinformatics analyses were employed predict genes and assign functional annotations. 12, which belongs Siphoviridae family, exhibited substantial potential, burst size 5.7 PFU/infected cells latent period 20 min. Host range determination experiments demonstrated effectiveness against faecalis strains, positioning precise agent. Stability assays underscore resilience across diverse environmental conditions. provides understanding composition, lytic lifecycle parameters, practical applications, particularly efficacy murine wound models. These results emphasize promising role therapy, specifically targeted approach strains. nuanced insights derived from this research will contribute ongoing pursuit efficacious therapies offer valuable implications addressing challenges associated infections.
Язык: Английский
Процитировано
8
Virology Journal, Год журнала: 2024, Номер 21(1)
Опубликована: Окт. 18, 2024
Dental infections, such as apical Periodontitis, periodontitis, and peri-implantitis (PI), are closely associated with specific bacterial species, including Streptococcus mutans (S. mutans), Porphyromonas gingivalis (P. gingivalis), Fusobacterium nucleatum (F. nucleatum), among others. Antibiotics extensively utilized for prophylactic therapeutic purposes in the treatment of dental infections other dental-related issues. Unfortunately, rapid emergence antimicrobial resistance has accompanied increased use antibiotics recent years. Specific pathogens have reached a critical stage antibiotic resistance, characterized by proliferation pan-resistant strains scarcity viable alternatives. Therapeutic particular bacteriophage (phage) particles that target is one potential alternative to now being seriously considered treating illnesses. A kind virus known phage capable infecting eliminating bacteria. Because they can't infect cells plants animals, phages might be harmless substitute antibiotics. To control oral disorders periodontitis caries, several research been conducted this area study identify from human saliva plaque. The capacity these agents disturb biofilms expands their effectiveness against plaque cases PI, periodontitis. This review summarizes current antibacterial properties used treat variety peri-implantitis, infected dentin,
Язык: Английский
Процитировано
7
Cancer Pathogenesis and Therapy, Год журнала: 2025, Номер unknown
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Annals of Clinical Microbiology and Antimicrobials, Год журнала: 2025, Номер 24(1)
Опубликована: Апрель 13, 2025
Endodontic treatment failures are predominantly attributed to Enterococcus faecalis (E. faecalis) infection, a Gram-positive coccus. E. forms biofilms, resist multiple antibiotics, and can withstand endodontic disinfection protocols. Vancomycin-resistant strains, in particular, challenging treat associated with serious medical complications. A novel phage, vB_EfaS_ZC1, was isolated characterized. Its lytic activity against assessed vitro through time-killing biofilm assays. The phage's stability under various conditions determined. Genomic analysis conducted characterize the phage its virulence. propolis, their combination were evaluated as an intracanal irrigation solution 4-week mature biofilm, using ex vivo infected human dentin model. antibiofilm analyzed colony-forming unit assay, field emission scanning electron microscopy, confocal laser microscopy. siphovirus prolate capsid, exhibited strong strains. In assays indicated effectiveness inhibiting planktonic growth disrupting biofilms. remained stable wide range of temperatures (- 80 60 °C), tolerated pH levels from 4 11; however viability significantly reduced after UV exposure. strongly suggests virulence suitability for therapeutic applications; neither lysogeny markers nor antibiotic resistance identified. Phylogenetic clustered vB_EfaS_ZC1 within genus Saphexavirus. both alone demonstrated potent effects compared conventional root canal irrigation. Phage demonstrates promising therapy, either individually or addressing infections caused by faecalis.
Язык: Английский
Процитировано
0
Topics in Catalysis, Год журнала: 2024, Номер unknown
Опубликована: Сен. 30, 2024
Язык: Английский
Процитировано
1
Microbiology Resource Announcements, Год журнала: 2024, Номер 13(10)
Опубликована: Сен. 10, 2024
ABSTRACT We report the genome sequences of 12 Enterococcus faecalis phages isolated in Kenya, belonging to genus Copernicusvirus , Efquatrovirus Saphexavirus and Kochikohdavirus . They have double-stranded DNA with lengths varying from 17,979 147,374 bp G+C content 33.14% 40.05%. The genomes contain 28–250 coding sequences.
Язык: Английский
Процитировано
0
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
0