Attenuating Vibrio harveyi Virulence Through Quorum Sensing Interference Using Piperine: An In Vitro and In Silico Approach
Journal of Fish Diseases,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 5, 2025
ABSTRACT
Aquaculture
diseases
caused
by
pathogens
such
as
Vibrio
spp.,
amplified
the
emergence
of
antibiotic
resistance,
threaten
aquaculture
industry.
Due
to
its
critical
role
in
regulating
expression
genes
related
resistance
and
virulence,
quorum
sensing
(QS)
was
proved
be
an
ideal
target
disease
control.
In
this
study,
we
investigated
effects
alkaloid
piperine
on
QS
system
a
fish
pathogenic
harveyi
strain.
vitro
assays
showed
that
inhibits
biofilm
formation
V.
without
affecting
their
growth.
Moreover,
specifically
reduced
activity
,
evident
inhibition
bioluminescence,
likely
through
AI‐2
pathway.
Molecular
docking
simulations
significant
binding
energies
between
proteins—LuxP,
LuxQ,
LuxR
LuxS—revealing
competitive
inhibitory
against
LuxP,
LuxR,
LuxS,
non‐competitive
interactions
with
LuxQ.
This
study
demonstrated
elucidated
mechanism
action
QS,
implying
potential
application
systems.
Язык: Английский
Cuminaldehyde synergistically enhances the antimicrobial and antibiofilm potential of gentamicin: A direction towards an effective combination for the control of biofilm-linked threats of Staphylococcus aureus
Brazilian Journal of Microbiology,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 12, 2025
Язык: Английский
Development of novel strategies against the threats of drug-resistant Escherichia coli: an in silico and in vitro investigation
3 Biotech,
Год журнала:
2025,
Номер
15(4)
Опубликована: Март 6, 2025
Язык: Английский
Cuminaldehyde in combination with tetracycline shows promising antibiofilm activity against drug-resistant Pseudomonas aeruginosa
Biofouling,
Год журнала:
2024,
Номер
unknown, С. 1 - 20
Опубликована: Ноя. 4, 2024
,
an
opportunistic
pathogen
often
causes
biofilm-linked
infections.
A
combinatorial
approach
involving
tetracycline
(antibiotic)
and
cuminaldehyde
(phytochemical)
was
explored
to
combat
this
infectious
pathogen.
The
results
showed
that
both
individually
demonstrated
antibacterial
effects.
However,
when
the
compounds
were
applied
together,
there
a
significant
increase
in
their
antimicrobial
potential.
determined
fractional
inhibitory
concentration
index
of
0.43
indicated
synergistic
interaction
between
two
compounds.
Furthermore,
series
experiments
combined
application
could
lead
enhancement
antibiofilm
This
enhanced
potential
attributed
accumulation
reactive
oxygen
species
increased
cell
membrane
permeability.
Besides,
reduced
secretion
various
virulence
factors
from
Язык: Английский
Antimicrobial Profiling of Piper betle L. and Piper nigrum L. Against Methicillin-Resistant Staphylococcus aureus (MRSA): Integrative Analysis of Bioactive Compounds Based on FT-IR, GC-MS, and Molecular Docking Studies
Separations,
Год журнала:
2024,
Номер
11(11), С. 322 - 322
Опубликована: Ноя. 8, 2024
This
study
explored
the
antimicrobial
potential
of
Piper
betle
L.
(PBL)
and
nigrum
(PNL)
extracts
against
MRSA.
Plant
parts
including
stem,
leaf,
fruit
were
extracted
using
aquadest,
methanol,
hexane,
resulting
in
18
distinct
extracts.
FT-IR
combined
with
cluster
analysis
(CA)
categorized
extracts,
anti-MRSA
activity
was
assessed
through
paper
disk
diffusion
method.
The
most
potent
further
analyzed
GC-MS
to
identify
bioactive
compounds.
Additionally,
molecular
docking
studies
conducted
for
MRSA
protein
targets
(4DKI,
6H5O,
4CJN).
hexane
extract
PNL
aqueous
PBL
showed
strongest
inhibitory
effects.
identified
piperine
(14.22%)
diisooctyl
phthalate
(14.67%)
as
major
compounds,
piperolein
B,
piperanine,
β-caryophyllene
oxide,
α-caryophylladienol
minor
compounds
PNL,
while
hydroxychavicol
(81.89%)
chavibetol
(12.01%)
predominant
aquadest
PBL.
Molecular
revealed
that
B
had
strong
binding
affinities
proteins
4DKI,
4CJN,
comparable
ciprofloxacin.
In
conclusion,
this
confirms
sources
novel
agents,
supporting
research
develop
new
therapies.
Язык: Английский