Drug-Eluting and Antibacterial Core–Shell Polycaprolactone/Pectin Nanofibers Containing Ti₃C₂T_x MXene and Medical Herbs for Wound Dressings

ACS Applied Bio Materials, Год журнала: 2024, Номер 7(11), С. 7244 - 7255

Опубликована: Ноя. 5, 2024

Fibrous scaffolds capable of delivering natural drugs and herbs show great promise for tissue regeneration wound care, particularly in personalized medicine. This study presents the fabrication characterization drug-eluting antibacterial core–shell mats composed polycaprolactone (PCL) pectin nanofibers produced through coaxial electrospinning. Berberine chloride (BBR), an herbal compound with antineoplastic, anti-inflammatory, antilipidemic, antidiabetic properties, served as model drug. Poly(vinyl alcohol) (PVA) was blended to enhance mechanical properties core fibers. The shell modified two-dimensional Ti3C2Tx (MXene) nanosheets subjected covalent ionic cross-linking. Structural analysis confirmed successful production bead-free fibers diameters ranging from 160 350 nm, depending on composition. PCL were uniformly coated a pectin/PVA approximately 90 nm thick. inclusion BBR MXene increased fiber diameter. Drug-release kinetics, modeled by using Korsmeyer-Peppas, revealed two-stage release mechanism. An initial burst occurred within first 24 h (kinetic exponent n = 1.36), followed sustained over 2 weeks (n 0.48). mechanisms identified case-II relaxational stage, transitioning quasi-Fickian diffusion second. Incorporating into further prolonged drug release. strength improved significantly factor 7 4 wet dry conditions, respectively. In vitro biocompatibility assays L929 cells demonstrated excellent cell attachment compatibility. Additionally, tests against Escherichia coli showed that enhanced activity 30%. These results suggest functional biocomposite hold potential developing innovative, dressings.

Язык: Английский

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Antibacterial activity and mechanism of Sodium houttuyfonate against heteroresistant Pseudomonas aeruginosa

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Март 6, 2025

Pseudomonas aeruginosa is a common gram-negative opportunistic pathogen that now commonly treated with carbapenems, such as Meropenem. However, the increasing rate of emergence heteroresistant strains poses therapeutic challenge. Therefore, we examined antibacterial activity Sodium houttuyfonate (SH, compound derived from Houttuynia cordata) in combination Meropenem (MEM) against and investigated mechanism houttuyfonate. Heteroresistant was used experimental strain for study combined action two drugs inves-tigated by determining Minimum Inhibitory Concentration (MIC), Fractional Index (FICI), time killing curves. Also effect on biofilm well bacterial swimming motility assay crystal violet staining biofilm, microanalysis assay, quantitative real-time PCR (qRT-PCR) population sensing related virulence factors. For screened strains, MIC SH 4,000 μg/ml; FICI both four ≤0.5, which showed synergistic effect. When ≥ 250 μg/ml, it able to effectively inhibit formation ability compared blank control group. In qRT-PCR experiment, expression formation-related genes (pslA, pelA, aglD, lasI, lasR, rhlA) ability-related (fliC, pilZ, pilA) were decreased SH-treated group, Our demonstrated exhibited inhibition aeruginosa, may achieve its inhibitory inhibiting formation, motility, down-regulating genes.

Язык: Английский

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Characterization of carbapenem-resistant Klebsiella pneumoniae in bloodstream infections: antibiotic resistance, virulence, and treatment strategies

Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 15

Опубликована: Март 7, 2025

Background Carbapenem-resistant Klebsiella pneumoniae (CRKP) infections pose a major clinical challenge due to multidrug resistance. This study evaluated the features, antibiotic resistance mechanisms, virulence factors, and potential therapeutic impact of berberine hydrochloride (a traditional Chinese medicine) in CRKP infections. Methods Ninety-four isolates from bloodstream at First Affiliated Hospital Zhejiang Medical University were characterized for carbapenemase genes, susceptibility, determinants. Clinical data analyzed identify risk factors infection, vitro antibacterial activity was assessed. Results Most (71.3%) intensive care unit (ICU) patients. The bla KPC gene predominant mechanism (62.77%), while genes uge (93.62%) wabG (92.55%) highly prevalent. ICU admission, male sex, respiratory diseases, invasive procedures, prior use third-generation cephalosporinase inhibitors, absence medicine treatment linked poorer outcomes. Importantly, inhibited growth , with minimum inhibitory concentration (MIC) 125 mg/mL. Conclusion Our reveals multifaceted profiles highlights value as an adjunctive agent. These findings support further investigations into incorporating improve outcomes patients

Язык: Английский

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A mitochondrial-targeted nanomedicine based on hollow mesoporous silica nanoparticles for enhanced colorectal cancer therapy

Colloids and Interface Science Communications, Год журнала: 2025, Номер 66, С. 100833 - 100833

Опубликована: Март 26, 2025

Язык: Английский

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Drugs Discovery from Traditional Phytotherapy

Опубликована: Апрель 29, 2025

BACKGROUND. Phytotherapy refers to the use of plant extracts for therapeutic purpose. It plays important role in ancient medicine practices. has been considered as a cornerstone development modern drugs. With advancement field science and technology, researchers systematically evaluate medicinal properties plants. OBJECTIVE. is an integrative approach that integrates traditional knowledge with techniques identification, isolation, test derivative compounds. Literature on plant-derived natural products used suitable precursors drug discovery was included. METHODS. The data source were obtained through literature publications using different scientific search engines. RESULTS. These papers carefully evaluated, critically analyzed structured accurate information. process begins ethnobotanical surveys study select promising potential herb. Phytochemical methods are then employed extract isolate active constituents. phytotherapy drugs have plants support health manage various conditions. Recently analytical chemistry genomics essential accelerating process. High-throughput screening, molecular docking, bioinformatics tools enabled rapid identification bioactive Traditional significantly influenced by providing wealth about plant-based remedies. Many pharmaceuticals roots herbal practices, compounds isolated from forming basis effective treatments. CONCLUSION. This integration wisdom research led targeted therapies, emphasizing importance discovery. As continue explore compounds, remains vital new medicines. Moreover, advancements pharmacokinetics pharmacodynamics improved understanding interact biological systems, enhancing their efficacy safety profiles.

Язык: Английский

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A nano-delivery system combining natural agents and phototherapy application enhances the combating of drug-resistant bacteria

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 163305 - 163305

Опубликована: Май 1, 2025

Язык: Английский

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