Bacteria-Inspired Synthesis of Silver-Doped Zinc Oxide Nanocomposites: A Novel Synergistic Approach in Controlling Biofilm and Quorum-Sensing-Regulated Virulence Factors in Pseudomonas aeruginosa

Antibiotics, Год журнала: 2025, Номер 14(1), С. 59 - 59

Опубликована: Янв. 9, 2025

Multidrug-resistant Pseudomonas aeruginosa infections pose a critical challenge to healthcare systems, particularly in nosocomial settings. This drug-resistant bacterium forms biofilms and produces an array of virulent factors regulated by quorum sensing. In this study, metal-tolerant bacteria were isolated from metal-contaminated site screened for their ability synthesize multifunctional nanocomposites (NCs). Rapid color changes the reaction solution evidenced biotransformation process. The potent Bacillus cereus SASAK, identified via 16S rRNA sequencing deposited GenBank under accession number MH885570, facilitated microbial-mediated synthesis ZnO nanoparticles silver-doped NCs. These biogenic characterized using UV-VIS-NIR spectroscopy, FTIR, XRD, zeta potential, HRTEM, FESEM, EDX analyses. At sub-MIC concentration 100 µg/mL, 2% Ag-ZnO NCs effectively inhibited factor production biofilm formation P. without affecting bacterial growth. Notably, there was significant reduction violacein pigment (96.25%), swarming motility, pyocyanin (1.87 µg/mL). Additionally, (81.1%) EPS (83.9%) substantially hindered, along with reduced extracellular protease activity, as indicated zone (from 2.3 1.8 cm). study underscores potential promising agents combating sensing-mediated virulence chronic caused multidrug-resistant aeruginosa.

Язык: Английский

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Uncovering the in vitro antibiofilm potential of Dittrichia graveolens silver nanoparticle against infectious agents

Preparative Biochemistry & Biotechnology, Год журнала: 2025, Номер unknown, С. 1 - 7

Опубликована: Март 1, 2025

Dittrichia graveolens (L.) Desf., an aromatic plant, is used in various treatments popular medicine. Especially its methanolic and aqueous extracts have many biological activities. Nanotechnology, which has pioneered important research recent years, also begun to be preferred the field of health. Based on this, we aim investigate effect silver nanoparticle synthesized from antimicrobial antibiofilm. We tried destroy biofilm structures responsible for antibiotic resistance bacteria with environmentally friendly nanoparticles. The activity AgNPs obtained through green synthesis, was evaluated by liquid medium microdilution method. MIC (minimum inhibitory concentration) values Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa Klebsiella pneumoniae strains were determined method 96-well ELISA plates. performed using crystal violet flat-bottom microplates. four standard 128 μg/mL. All showed antibiofilm effects every concentration AgNPs. lowest inhibition detected as about 90% at 16 It concluded that effective bacteria. In addition, this first study subject. This may innovative approach scientific world a herbal bacterial perspective.

Язык: Английский

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Exploring the antivirulence mechanisms of ZnO-PEG-quercetin nanoparticles: Biofilm disruption, attenuation of virulent factors, and cell invasion suppression against pathogenic Pseudomonas aeruginosa

Bioorganic Chemistry, Год журнала: 2025, Номер 161, С. 108527 - 108527

Опубликована: Апрель 26, 2025

Язык: Английский

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Discovery of novel DdlA inhibitors in multidrug-resistant Pseudomonas aeruginosa using virtual screening, molecular docking, and dynamics simulations

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Май 1, 2025

Язык: Английский

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