Springer eBooks, Год журнала: 2024, Номер unknown, С. 607 - 641
Опубликована: Янв. 1, 2024
Язык: Английский
Annals of Microbiology, Год журнала: 2023, Номер 73(1)
Опубликована: Апрель 10, 2023
Abstract Purpose The textile industry’s previous chemical use resulted in thousands of practical particulate emissions, such as machine component damage and drainage system blockage, both which have implications. Enzyme-based processing is cost-effective, environmentally friendly, non-hazardous, water-saving. purpose this review to give evidence on the potential activity microbial cellulase industry, mostly confined realm research. Methods This was progressive by considering peer-reviewed papers linked production, its prospective application for industries appraised produced develop assessment. Articles were divided into two categories based results trustworthy educational journals: methods used produce diversity microorganisms through fermentation processes approaches microbes fermentation. Submerged (SMF) solid-state (SSF) techniques are currently being meet industrial demand production bio industry. Results Microbial vital increasing day due no side effect environment human health becoming increasingly important. In conventional processing, gray cloth subjected a series treatments that involved breaking dye molecule’s amino group with Cl − , started accelerated dye(-resistant) bond cracking. A enzyme primarily derived from variety species found various ecological settings biotextile/bio-based product technology future needs applications. Conclusion Cellulase has been advantages cellulose-based textiles, well quality enhancement fabric maintenance over traditional approaches. Cellulase’s role industry chosen an appropriate sound solution long healthy lifestyle.
Язык: Английский
Процитировано
50
Bioresource Technology, Год журнала: 2023, Номер 385, С. 129449 - 129449
Опубликована: Июль 4, 2023
Язык: Английский
Процитировано
30
Systems Microbiology and Biomanufacturing, Год журнала: 2025, Номер unknown
Опубликована: Март 3, 2025
Язык: Английский
Процитировано
1
Mycology: An International Journal on Fungal Biology, Год журнала: 2023, Номер 14(2), С. 75 - 90
Опубликована: Янв. 10, 2023
Nematode-trapping fungi (NTF) are the majority of carnivorous microbes to capture nematodes through diverse and sophisticated trapping organs derived from hyphae. They can adopt lifestyles in addition saprophytism obtain extra-nutrition nematodes. As a special group fungi, NTF not only excellent model organism for studying lifestyle transition but also natural resources exploring biological control However, mechanism remains poorly understood. Nowadays, omics studies have provided numerous genes pathways that associated with phenotypes traits, which need molecular tools verify. Here, we review development progress gene manipulation NTF, including methodology strategy transformation, random mutagenesis methods target methods. The principle practical approach each method was summarized discussed, basic operational flow tool described. This paper offers clear reference instruction researchers who work on as well other fungi.
Язык: Английский
Процитировано
11
Microbiological Research, Год журнала: 2025, Номер 294, С. 128097 - 128097
Опубликована: Фев. 14, 2025
Myceliophthora thermophila is a thermophilic fungus, known to produce industrially important enzymes in biorefineries. The mechanism underlying cellulase and xylanase expression filamentous fungi complex regulatory network controlled by numerous transcription factors (TFs). These TFs M. remain unclear. Here, we identified characterised novel regulator MtFKH1 through comparative transcriptomic genetic analyses. Five of the eight potential TFs, which showed differential levels when grown on Avicel glucose, were successfully deleted using newly designed CRISPR/Cas9 system. This system forkhead TF MtFKH1. disruption Mtfkh1 elevated cellulolytic xylanolytic enzyme activities, whereas overexpression led considerable decrease production cultivated Avicel. loss also exhibited an impairment sporulation thermophila. Real-time quantitative reverse PCR (RT-qPCR) electrophoretic mobility shift assays (EMSAs) demonstrated that regulates gene specifically bind promoter regions genes encoding β-glucosidase (bgl1/MYCTH_66804), cellobiohydrolase (cbh1/MYCTH_109566), (xyn1/MYCTH_112050), respectively. Furthermore, DNase I footprinting analysis binding motif upstream region Mtbgl1, with strongest affinity. Finally, profiling Gene Ontology (GO) enrichment analyses deletion mutant revealed regulon significantly prevalent hydrolase activity (acting glycosyl bonds), polysaccharide binding, carbohydrate metabolic process functional categories. findings expand our knowledge how factor lignocellulose degradation provide target for engineering fungal cell factories hyperproduction xylanase.
Язык: Английский
Процитировано
0
Organogenesis, Год журнала: 2025, Номер 21(1)
Опубликована: Апрель 5, 2025
Fiber-photometric is a novel optogenetic method for recording neural activity in vivo, which allows the use of calcium indicators to observe and study relationship between behavior free-ranging animals. Calcium also convert changes concentration cells or tissues into recordable fluorescent signals, can then be observed using system fiber-photometric. To date, there paucity relevant literature on proper selection application fiber-photometric indicators. Therefore, this paper will detail how correctly select apply fiber-photometer four sections: basic principle optical fiber photometry, probes viral vector systems, measurement specific expression proteins tissues. correct suitable optic greatly assist researchers exploring link neuronal neuropsychiatric disorders.
Язык: Английский
Процитировано
0
Cells, Год журнала: 2023, Номер 12(11), С. 1547 - 1547
Опубликована: Июнь 5, 2023
Fungi are an important group of microorganisms that play crucial roles in a variety ecological and biotechnological processes. depend on intracellular protein trafficking, which involves moving proteins from their site synthesis to the final destination within or outside cell. The soluble N-ethylmaleimide-sensitive factor attachment receptors (SNARE) vital components vesicle trafficking membrane fusion, ultimately leading release cargos target destination. v-SNARE (vesicle-associated SNARE) Snc1 is responsible for anterograde retrograde between plasma (PM) Golgi. It allows fusion exocytic vesicles PM subsequent recycling Golgi-localized back Golgi via three distinct parallel pathways. This process requires several components, including phospholipid flippase (Drs2-Cdc50), F-box (Rcy1), sorting nexin (Snx4-Atg20), retromer submit, COPI coat complex. interacts with SNAREs (Sso1/2, Sec9) complex complete exocytosis. also endocytic (Tlg1 Tlg2) during trafficking. has been extensively investigated fungi found various aspects When overexpressed alone combination some key secretory it results enhanced production. article will cover role its interactions other efficient cellular transportation.
Язык: Английский
Процитировано
10
Fermentation, Год журнала: 2024, Номер 10(7), С. 361 - 361
Опубликована: Июль 16, 2024
Bioethanol is the most commonly used biofuel. It an alternative to replace fossil fuels in renewable energy; it can be produced from lignocellulosic feedstock using a biotechnological process. Their participation of microorganisms crucial bioconversion process fermentation for ethanol production and involve bacteria, fungi, yeasts. However, when working within bioethanol processes lignocellulose feedstock, face some challenges, such as high temperature, solids content, ability ferment sugars concentration. Such challenges will depend on operative strategies, simultaneous saccharification fermentation, separate hydrolysis semi-simultaneous consolidated bioprocessing; these are common configurations. This review presents different trends microbial role, biochemical application, strategies second generation.
Язык: Английский
Процитировано
3
Industrial Crops and Products, Год журнала: 2024, Номер 222, С. 119432 - 119432
Опубликована: Авг. 28, 2024
Язык: Английский
Процитировано
3
Scientific Reports, Год журнала: 2022, Номер 12(1)
Опубликована: Ноя. 14, 2022
Trichoderma reesei is a widely used host for producing cellulase and hemicellulase cocktails lignocellulosic biomass degradation. Here, we report genetic modification strategy industrial T. that enables enzyme production using simple glucose without inducers, such as cellulose, lactose sophorose. Previously, the mutated XYR1V821F or XYR1A824V was known to induce xylanase only carbon source, but its composition biased toward xylanases, performance insufficient degrade lignocellulose efficiently. Therefore, examined combinations of constitutively expressed CRT1, BGLR, VIB1, ACE2, ACE3, regulators essential factors expression E1AB1 strain has been highly mutagenized improving productivity expressing ß-glucosidase high performance. The results showed ACE3 promoted expression. Furthermore, co-expression these two transcription also resulted in increased productivity, with 1.5-fold higher than conventional single XYR1V821F. Additionally, 5.5-fold compared an enhanced ACE3. Moreover, although DNA-binding domain had considered inducer-free production, found partially truncated more effective when co-expressed This study demonstrates factors, optimized productivity.
Язык: Английский
Процитировано
14