Evaluation of SARS-CoV-2 Seroprevalence and Variant Distribution During the Delta–Omicron Transmission Waves in Greater Accra, Ghana, 2021

Viruses, Год журнала: 2025, Номер 17(4), С. 487 - 487

Опубликована: Март 28, 2025

A significant proportion of SARS-CoV-2 infections in Africa were identified as asymptomatic. With the surge Omicron variant, asymptomatic participants epidemiological surveys key to accurately estimating seroprevalence and true population. This study assessed seroprevalence, active infections, circulating variants Accra, Ghana, during wave. Secondary objectives included assessing association between sociodemographic factors, vaccination, adherence recommended prevention control measures. We conducted a cross-sectional survey Greater Accra December 2021 using standardized questionnaire. Serum naso-oropharyngeal swab samples collected from 1027 individuals aged ≥ 5 years for estimation total antibodies detection infection. The found an overall 86.8% [95% CI: 84.53–88.77]. PCR test positivity was 10%, with Delta accounting 44.1% 8.8% respectively. Vaccination (cOR = 10.5, 95% 4.97–26.9, p < 0.001) older age, particularly 60+ age group 6.05, 2.44–20.2, 0.001), associated increase odds seropositivity among participants. High indication high exposure transmission rates and/or vaccine-induced seroprevalence.

Язык: Английский

---

Clinical characteristics and prognosis of COVID-19- associated invasive pulmonary aspergillosis in critically patients: a single-center study

Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 15

Опубликована: Апрель 22, 2025

Objective A single-center retrospective study was conducted according to the latest diagnostic criteria of European Consortium for Mycology in Medicine/International Society Human and Animal Mycoses (ECMM/ISHAM) Consensus, which describes clinical characteristics, factors influencing prognosis a group patients with COVID-19 (Omicron variant) combined invasive pulmonary mycoses onset disease at end 2022. Methods This retrospectively analyzed data related 58 hospitalized severe pneumonia due infection admitted ICU critical care medicine, respiratory ICU, Department Infections First Affiliated Hospital Soochow University from December 1, 2022, January 31, 2023. CAPA defined ECMM/ISHAM consensus criteria. Our compared microbiological characteristics associated risk fungal infections performed univariate multivariate analyses mortality COVID-19-Associated Pulmonary Aspergillosis (CAPA). Results 17 (29.3%) critically ill were diagnosed CAPA, 10 (58.82%) Probable 7 (41.18%) Possible CAPA. Among this Aspergillus strains, fumigatus strains found 13 cases (76.47%) niger 4 (23.53%). had concomitant bacterial rate 57.14% (4/7), Acinetobacter baumannii most common pathogen. galactomannan assay bronchoalveolar lavage fluid (BALF) 5 100% (5/5) positivity rate, two or more serum (GM) assays patients, probability favorable results both 41.2% (7/17). The 60-day 52.9% (9/17), whereas non-CAPA 24.4% (10/41), statistically different (P = 0.035). Diabetes mellitus 0.018, OR: 5.040 (95% CI: 1.314-19.337)), renal insufficiency (P=0.002, 11.259 2.480-51.111)), chronic obstructive (COPD) 0.003, 6.939 1.963-24.531)), elevated interleukin-6 (IL-6) 0.022, 4.160 1.226~14.113)), mechanical ventilation 0.002, 8.100 (95%CI: 2.132~30.777)), increased duration steroids use 1.071 1.010~1.135)), cumulative dose ((P &lt; 0.001, 1.012 1.009~1.015)), tocilizumab 0.020, 11.480 2.480~51.111)), length hospitalization 0.021, 1.038 1.006 1.071)), increase type antibiotics used 1.603 1.181 2.176)) occurrence infections, not, baricitinib hypertension did not have significant effect on &gt; 0.05). Patients higher their prolonged patients. all-cause 52.9%. We also potential mortality, including 0.040 10.500, 1.115 98.914)), advanced age 0.043 1.212, 1.460)), significantly CRP level 0.042 1.043, 1.002~1.078)) worse prognosis. Steroids use, gender, diabetes patient death

Язык: Английский

---

Differential responses of lung and intestinal microbiota to SARS-CoV-2 infection: a comparative study of the Wuhan and Omicron strains in K18-hACE2 Tg mice

Laboratory Animal Research, Год журнала: 2025, Номер 41(1)

Опубликована: Апрель 23, 2025

Abstract Background The COVID-19 pandemic, caused by SARS-CoV-2, has led to the emergence of viral variants with distinct characteristics. Understanding differential impacts SARS-CoV-2 is crucial for effective public health response and treatment development. We investigated effects original Wuhan strain emergent Omicron variant using a K18-hACE2 transgenic mouse model. compared mortality rates, loads, histopathological changes in lung tracheal tissues, as well alterations intestinal microbiota following infection. Results Our findings revealed significant differences between variants, causing higher severe pathology, elevated loads variant. Microbiome analyses uncovered novel shifts associated each variant, providing evidence variant-specific microbiome alterations. These suggest microbiome-related mechanisms that might modulate disease severity host responses Conclusions This study highlights critical terms mortality, changes, emphasizing role influencing outcomes. Novel include identification shifts, which underscore potential underlying severity. insights pave way future research exploring microbiome-targeted interventions mitigate other infections.

Язык: Английский

---

An update on SARS-CoV-2 immunization and future directions

Frontiers in Pharmacology, Год журнала: 2023, Номер 14

Опубликована: Март 9, 2023

Millions of people have died as a result SARS-CoV-2, which was first discovered in China and has since spread globally. Patients with SARS-CoV-2 infection may show range symptoms, including fever, coughing, shortness breath, or they no symptoms at all. To treat COVID-19 avoid serious infections, many medications vaccinations been employed. However, to entirely eradicate from the world, next-generation vaccine research is required because devastating consequences it having for humanity every nation’s economy. Scientists are working hard this dangerous virus across world. also undergone significant mutation, leading distinct viral types such alpha, beta, gamma, delta, omicron variants. This sparked discussion about effectiveness current vaccines newly formed A proper comparison these compare their efficacy number immunized against globally increases. Population-level statistics evaluating capacity reduce therefore being developed. In paper, we analyze on market terms production process, price, dosage needed, efficacy. article discusses challenges achieving herd immunity, likelihood reinfection, importance convalescent plasma therapy reducing infection.

Язык: Английский

---

In Silico and In Vitro Analyses of Multiple Terpenes Predict Cryptotanshinone as a Potent Inhibitor of the Omicron Variant of SARS-CoV-2

Processes, Год журнала: 2024, Номер 12(1), С. 230 - 230

Опубликована: Янв. 21, 2024

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant (B.1.1.529) underwent a substantial number of alterations, and the accompanying structural mutations in spike protein prompted questions about virus’s propensity to evade antibody neutralization produced by prior infection or vaccination. New SARS-CoV-2 have raised serious concerns regarding effectiveness drugs vaccines against virus; thus, identifying developing potent antiviral medications is crucial combat viral infections. In present study, we conducted detailed silico investigation that involves molecular docking, density functional (DFT) analysis, dynamics (MD) simulations, pharmacological analysis followed an vitro study with protein. Among fifty terpenes screened, cryptotanshinone saikosaponin B2 were found be S1-RBD inhibitors, displaying considerable hydrogen bond interactions key binding site residues, significant affinity, high reactivity attributed band gap energy. addition, 100 ns simulations further substantiated these findings, showcasing stability compounds within biological environment. With favorable pharmacokinetic properties low half inhibitory concentration (IC50) 86.06 ± 1.56 μM, inhibited variant. Our findings account for in-depth research on as inhibitor.

Язык: Английский

---

An exploration of adjusted flight operations affecting passenger load factors in the post-pandemic recovery

Research in Transportation Business & Management, Год журнала: 2024, Номер 55, С. 101144 - 101144

Опубликована: Май 29, 2024

Язык: Английский

---

A Review on the Impact of the SARS-CoV-2 Omicron Subvariant on Elderly Patients with Diverse Co-Morbidities

Biologics, Год журнала: 2023, Номер 3(2), С. 138 - 157

Опубликована: Июнь 9, 2023

The SARS-CoV-2 virus has caused a catastrophic impact on the world for past 3 years. now returned with emergence of Omicron (B.1.1.529) variant. Within two months its first in South Africa, became most dominating variant around world, being cause majority new infections at present. presented greatest transmission rate all previous variants despite presence mass vaccinations and acquired immunity. Several monoclonal antibodies mRNA vaccines have failed to produce desired effects owing large number mutations present introduction booster dose proven be great addition therapeutic armamentarium against Immunocompromised patients including elderly, cancer patients, organ transplant recipients, those multiple comorbidities been greater risk developing severe diseases since pre-Omicron era. again raised threat this population. protection from disease mortality rates through utilization immunizations controversial subgroup patients. Thus, designing large-scale studies evaluate effectiveness these can provide evidence-based recommendations improve survival This article attempts discuss different subvariants Omicron, differences mutational aspects along particular focus consequences infection elderly population diverse comorbidities.

Язык: Английский

---

CLINICAL CHARACTERISTICS OF PATIENTS WITH SEVERE AND CRITICAL COVID-19

Bulletin of Problems Biology and Medicine, Год журнала: 2023, Номер 1(3), С. 184 - 184

Опубликована: Янв. 1, 2023

Connection of the publication with planned research

Язык: Английский

---

B₅N₁₀ Nanocarrier Functionalized with Al, C, Si Atoms: A Drug Delivery Method for Infectious Disease Remedy

OBM Genetics, Год журнала: 2024, Номер 08(01), С. 1 - 19

Опубликована: Фев. 21, 2024

As proof has recommended a close connection between COVID-19 and neurodegenerative disorders, this article aims to investigate the chloroquine (CLQ) drug as SARS-CoV-2’s primary protease, which can prevent in vitro viral duplication of all diverse experiments present. CLQ is an anti-viral enlarged by Pfizer, operate orally effective 3C-like protease inhibitor. In study, been assessed for its effectiveness against coronavirus trapping it within boron nitride nanocage (B₅N₁₀_NC) functionalized with specific atoms delivery. This procedure relies on principle direct electron transfer be elucidated using density functional theory (DFT) quantum mechanics methods. It was performed theoretical method B3LYP/6-311+G(d,p) account aptitude B₅N₁₀_NC grabbing via electronic states, nuclear quadrupole resonance, magnetic thermodynamic specifications. Finally, resulting amounts illustrated that aluminum (Al), carbon (C), silicon (Si) adsorbing towards formation CLQ@Al–B₄N₁₀_NC, CLQ@C–B₄N₁₀_NC, CLQ@Si–B₄N₁₀_NC might provide reasonable formula delivery technique fulfilled computations due physicochemical properties PDOS, NMR, NQR, IR spectrum. An overview recent developments nanocage-based systems will provided, including design nanocages atom-doped nanocages.

Язык: Английский

---

From intramuscular to nasal: unleashing the potential of nasal spray vaccines against coronavirus disease 2019

Clinical & Translational Immunology, Год журнала: 2024, Номер 13(5)

Опубликована: Янв. 1, 2024

Abstract Coronavirus disease 2019, caused by severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), has affected 700 million people worldwide since its outbreak in 2019. The current pandemic strains, including Omicron and large subvariant series, exhibit strong transmission stealth. After entering the human body, virus first infects nasal epithelial cells invades host through angiotensin‐converting enzyme receptor transmembrane serine protease on cell surface. cavity is an important body part that protects against virus. Immunisation of mucosa produces immunoglobulin A antibodies effectively neutralise viruses. Saline irrigation, a type physical therapy, can reduce viral load prevent infections to some extent. As commonly used means fight SARS‐CoV‐2, intramuscular (IM) vaccine induce produce systemic immune response G antibody; however, antibody difficult distribute time cannot achieve good preventive effect. Intranasal (IN) vaccines compensate for shortcomings IM vaccines, mucosal responses, have better effect preventing infection. In this review, we discuss defence barrier, harm mechanism invasion into cells, cleaning, IN suggest increasing development use as supplement vaccines.

Язык: Английский

---