Resuscitation of viable but nonculturable microorganisms: A highly promising strategy for enhanced bioremediation

Critical Reviews in Environmental Science and Technology, Год журнала: 2025, Номер unknown, С. 1 - 24

Опубликована: Фев. 11, 2025

Язык: Английский

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The Viable but Non-Culturable (VBNC) State, a Poorly Explored Aspect of Beneficial Bacteria

Microorganisms, Год журнала: 2023, Номер 12(1), С. 39 - 39

Опубликована: Дек. 25, 2023

Many bacteria have the ability to survive in challenging environments; however, they cannot all grow on standard culture media, a phenomenon known as viable but non-culturable (VBNC) state. Bacteria commonly enter VBNC state under nutrient-poor environments or stressful conditions. This review explores concept of state, providing insights into beneficial employ this strategy. The investigation covers different chemical and physical factors that can induce latency cell features, gene expression observed cells also significance applications bacteria, methods evaluating bacterial viability, persist associated with higher organisms, facilitate return culturable Knowledge about capable entering remains limited; could face adverse environmental conditions when become suitable continue exert their effects. Likewise, unique feature positions them potential candidates for healthcare applications, such use probiotic enhance human health, industrial microbiology production prebiotics functional foods, beer wine industry. Moreover, formulations increase crop yields bioremediation offers an alternative pathway harness attributes.

Язык: Английский

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Advancements and perspectives of antimicrobial effects of combination mode nonthermal strategies - A review

Food Bioscience, Год журнала: 2025, Номер unknown, С. 106215 - 106215

Опубликована: Фев. 1, 2025

Язык: Английский

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Bacterial antioxidant mechanism in calcium peroxide aided sludge anaerobic fermentation

Bioresource Technology, Год журнала: 2023, Номер 384, С. 129327 - 129327

Опубликована: Июнь 15, 2023

Язык: Английский

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GP-fatty acid axis regulating the adaptive morphogenesis of VBNC bacteria in response to chlorine stress in the process of chlorination disinfection

Journal of Water Process Engineering, Год журнала: 2024, Номер 59, С. 104995 - 104995

Опубликована: Фев. 16, 2024

Язык: Английский

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Living in mangroves: a syntrophic scenario unveiling a resourceful microbiome

BMC Microbiology, Год журнала: 2024, Номер 24(1)

Опубликована: Июнь 28, 2024

Abstract Background Mangroves are complex and dynamic coastal ecosystems under frequent fluctuations in physicochemical conditions related to the tidal regime. The variation organic matter concentration, nutrients, oxygen availability, among other factors, drives microbial community composition, favoring syntrophic populations harboring a rich diverse, stress-driven metabolism. known for their carbon sequestration capability, integrated metabolic activity is essential global biogeochemical cycling. Here, we present reconstruction based on genomic functional capability flux profile between sympatric MAGs co-assembled from tropical restored mangrove. Results Eleven were assigned six Bacteria phyla, all distantly available reference genomes. showed several potential coupling points shortcuts complementary routes predicted interactions. Two scenarios drawn: heterotrophic scenario with plenty of sources an autotrophic limited or inhibitory conditions. sulfur cycle was dominant over methane major pathways identified acetate oxidation coupled sulfate reduction, acetogenesis carbohydrate catabolism, ethanol production fixation. Interestingly, gene sets similar those described wastewater effluent treatment processes identified. Conclusion mangrove reflected flexibility required survive fluctuating environments as microhabitats created by regime sediments. components strongly suggest that communities could represent resourceful model biotechnological applications occur naturally environment.

Язык: Английский

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Regulation of microbial activity based on quorum sensing: Implications for biological wastewater treatment

International Biodeterioration & Biodegradation, Год журнала: 2025, Номер 199, С. 106029 - 106029

Опубликована: Фев. 23, 2025

Язык: Английский

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Environmental matrix and moisture influence soil microbial phenotypes in a simplified porous media incubation

mSystems, Год журнала: 2025, Номер unknown

Опубликована: Фев. 24, 2025

ABSTRACT Soil moisture and porosity regulate microbial metabolism by influencing factors, such as system chemistry, substrate availability, soil connectivity. However, accurately representing the environment establishing a tractable community that limits confounding variables is difficult. Here, we use reduced-complexity consortium grown in glass bead porous media amended with chitin to test effects of structural matrix on phenotypes. Leveraging metagenomes, metatranscriptomes, metaproteomes, metabolomes, saw our significantly altered phenotypes compared liquid incubations, denoting importance incorporating pores surfaces for understanding soils. These phenotypic shifts were mainly driven differences expression Streptomyces Ensifer , which included significant decrease overall degradation between liquid. Our findings suggest success likely related its ability repurpose carbon via glyoxylate shunt amidst lack byproducts while potentially using polyhydroxyalkanoate granules C source. We also identified traits expressed others, including motility, stress resistance, conservation, influence metabolic profiles observed across treatments. Together, these results demonstrate incubations promote structure-induced are better proxy conditions than culture systems. Furthermore, they emphasize encompass not only multi-enzyme pathways involved but include complex interactions other members. IMPORTANCE critical shaping metabolism. laboratory experiments remains challenging frontier. Through reduced complexity experiment media, reveal predicting from gene-based alone often falls short capturing intricate cellular interactions. highlight affect decomposition, environmental (i.e., beads) shifting towards tolerance, resource acquisition, increased ultimately invoking unique strategies evident cultures. Moreover, find evidence changes relate regarding transporters, biofilm formation, degradation. Ultimately, showcase how communities can be informative present useful alternative cultures studying

Язык: Английский

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Quorum sensing luxI/R genes enhances cadmium detoxification in Aeromonas by up-regulating EPS production and cadmium resistance genes

Journal of Hazardous Materials, Год журнала: 2025, Номер unknown, С. 137959 - 137959

Опубликована: Март 1, 2025

Язык: Английский

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The response to desiccation in Acinetobacter baumannii

Virulence, Год журнала: 2025, Номер 16(1)

Опубликована: Апрель 12, 2025

The long-term resistance to desiccation on abiotic surfaces is a key determinant of the adaptive success Acinetobacter baumannii as healthcare-associated bacterial pathogen. Here, cellular and molecular mechanisms enabling A. resist persist were investigated. Experiments set up mimic response air-drying that would occur when cells contaminate fomites in hospitals. Resistance transition "viable but nonculturable" (VBNC) state determined laboratory-adapted strain ATCC 19606T epidemic ACICU. Culturability, membrane integrity, metabolic activity, virulence, gene expression profile compared between two strains at different stages desiccation. Upon desiccation, ACICU lose culturability lower their metabolism, enter VBNC state. However, desiccated fully recover virulence an insect infection model following rehydration physiological buffers or human biological fluids. Transcriptome chemical analyses during unveiled production protective metabolites (L-cysteine L-glutamate) decreased energetic metabolism consequent activation glyoxylate shunt (GS) pathway, confirmed by reduced resuscitation efficiency aceA mutants, lacking enzyme GS pathway. cell formation extensive reprogramming provide basis for with implications environmental control measures aimed preventing transmission

Язык: Английский

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