Efficient Production of the Colorless Carotenoid Phytoene in Yarrowia lipolytica through Metabolic Engineering DOI

Kwanghyun Park,

Gyeongmin Kim,

Sungman Cha

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 19, 2024

Phytoene, a colorless carotenoid with unique ultraviolet (UV)–B absorption properties, offers potential for applications in functional food, cosmetics, and therapeutics. However, their low natural yield poses challenge large-scale production. This study aims to enhance phytoene production the oleaginous yeast Yarrowia lipolytica by introducing heterologous synthase gene combined metabolic engineering approaches. We enhanced synthesis overexpressing key genes mevalonate pathway compartmentalizing biosynthetic within peroxisomes. Moreover, we inhibited glyoxylate cycle increase accumulation of peroxisomal acetyl-CoA available Our engineered strains demonstrated significant production, reaching up 1.34 g/L titer 58.74 mg/gDCW flask-scale fed-batch culture, which are highest levels reported date. These results underscore Y. as robust platform producing phytoenes other terpenoids on an industrial scale, offering valuable insights future efforts engineering.

Язык: Английский

Heterologous Biosynthesis of Terpenoids in Saccharomyces cerevisiae DOI
Junyang Wang, Xu Ji, Yi Rong

и другие.

Biotechnology Journal, Год журнала: 2025, Номер 20(1)

Опубликована: Янв. 1, 2025

ABSTRACT Terpenoids are widely distributed in nature and have various applications healthcare products, pharmaceuticals, fragrances. Despite the significant potential that terpenoids possess, traditional production methods, such as plant extraction chemical synthesis, face challenges meeting current market demand. With advancement of synthetic biology metabolic engineering, it becomes feasible to construct efficient microbial cell factories for large‐scale terpenoids. This article primarily centers on heterologous expression Saccharomyces cerevisiae , detailing terpenoid biosynthesis pathways through utilization cellular microcompartments, strategies key P450 enzymes synthesis pathway, regulation optimization host metabolism enhance flux synthesis. Additionally, we analyze propose solutions further refine yeast chassis more effective production.

Язык: Английский

Процитировано

0
Development of the autonomous lab system to support biotechnology research DOI Creative Commons
Keiji Fushimi, Yusuke Nakai,

Akiko Nishi

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 24, 2025

In this study, we developed the autonomous lab (ANL), which is a system based on robotics and artificial intelligence (AI) to conduct biotechnology experiments formulate scientific hypotheses. This was designed with modular devices Bayesian optimization algorithms, allowing it effectively run closed loop from culturing preprocessing, measurement, analysis, hypothesis formulation. As case used ANL optimize medium conditions for recombinant Escherichia coli strain, overproduces glutamic acid. The results demonstrated that our successfully replicated experimental techniques, such as sample preparation data improved both cell growth rate maximum growth. offers versatile scalable solution various applications in field of bioproduction, potential improve efficiency reliability processes future.

Язык: Английский

Процитировано

0
Use and dual use of synthetic biology DOI Creative Commons
Antoine Danchin

Comptes Rendus Biologies, Год журнала: 2025, Номер 348(G1), С. 71 - 88

Опубликована: Март 7, 2025

Язык: Английский

Процитировано

0
Introduction of human m6Am methyltransferase PCIF1 facilitates the biosynthesis of terpenoids in Saccharomyces cerevisiae DOI Creative Commons
Guoli Wang, Mingkai Li,

Baochen Fan

и другие.

Microbial Cell Factories, Год журнала: 2025, Номер 24(1)

Опубликована: Апрель 2, 2025

Язык: Английский

Процитировано

0
Systematic Engineering for Efficient Uric Acid-Degrading Activity in Probiotic Yeast Saccharomyces boulardii DOI Creative Commons

Wenzhuo Wang,

Lei Pan,

Hailin He

и другие.

ACS Synthetic Biology, Год журнала: 2025, Номер unknown

Опубликована: Май 8, 2025

Hyperuricemia, caused by uric acid disequilibrium, is a prevalent metabolic disease that most commonly manifests as gout and closely associated with spectrum of other comorbidities such renal disorders cardiovascular diseases. While natural engineered probiotics promote catabolism in the intestine have shown promise relieving hyperuricemia, limitations strain efficiency requirements for achieving high performance remain major hurdles practical application probiotic-mediated prevention management. Here, we employed systematic strategy to engineer high-efficiency pathway S. cerevisiae. An uricase from Vibrio vulnificus, exhibiting high-level activity cerevisiae, was identified acid-degrading component. The expression level stability urate transporter UapA were improved constructing chimera, enabling reliable import Additionally, constitutive promoters selected combinatorially assembled two functional components, creating collection pathways confer varied levels catabolic best-performing can express up 365.32 ± 20.54 μmol/h/OD, requiring only simple cultivation steps. Eventually, took advantage genetic similarity between model organism cerevisiae probiotic boulardii integrated optimized into high-expression integration loci genome. be stably maintained under high-density fermentation conditions. Overall, this study provided high-potential hyperuricemia-managing yeast strain, demonstrating capabilities developing recombinant probiotics.

Язык: Английский

Процитировано

0
Enhancing Cordycepin Biosynthesis in Yarrowia lipolytica via Lipid Droplets Compartmentalization Engineering and Optimized Fermentation Strategies DOI
Xiyu Duan,

Li-Ping Song,

Qing Jin

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Май 14, 2025

Cordycepin, a physiologically active nucleoside compound with broad applications in healthcare, is biosynthesized Cordyceps militaris through protein complex formed by CmCns1 and CmCns2. To enhance cordycepin heterologous production Yarrowia lipolytica, this study confirmed the colocalization of CmCns2 on lipid droplets, dominating process recruiting from cytoplasm to droplets via strong interactions. Critical lipid-droplet-targeting motifs within were identified. On basis, an engineered strain YL-CD3 was developed expanding CmCns3-NK compartmentalization. Then, fermentation parameters optimized increase yield 2008.23 mg/L shake flasks. Finally, fed-batch 2.4 L bioreactor for 144 h achieved 4780.75 (150.1 mg/OD600 66.57 mg/g glucose), marking highest reported titer Y. lipolytica. This work establishes lipolytica as high-potential platform efficient biosynthesis.

Язык: Английский

Процитировано

0
Recent advances in bio-based production of organic acids by genetically engineered yeasts DOI Creative Commons
Ning Xu, Haiyan Gao,

Yanran Wang

и другие.

Biochemical Engineering Journal, Год журнала: 2024, Номер unknown, С. 109587 - 109587

Опубликована: Ноя. 1, 2024

Язык: Английский

Процитировано

2
Engineering microbial cell factories by multiplexed spatiotemporal control of cellular metabolism: Advances, challenges, and future perspectives DOI

Wenwen Yu,

Ke Jin, Xianhao Xu

и другие.

Biotechnology Advances, Год журнала: 2024, Номер 79, С. 108497 - 108497

Опубликована: Дек. 5, 2024

Язык: Английский

Процитировано

1
A Rewired NADPH-Dependent Redox Shuttle for Testing Peroxisomal Compartmentalization of Synthetic Metabolic Pathways in Komagataella phaffii DOI Creative Commons
Albert Fina, Sílvia Àvila-Cabré, Elene Pereira

и другие.

Microorganisms, Год журнала: 2024, Номер 13(1), С. 46 - 46

Опубликована: Дек. 30, 2024

The introduction of heterologous pathways into microbial cell compartments offers several potential advantages, including increasing enzyme concentrations and reducing competition with native pathways, making this approach attractive for producing complex metabolites like fatty acids alcohols. However, measuring subcellular these remains technically challenging. Here, we explored 3-hydroxypropionic acid (3-HP), readily quantifiable sharing the same precursors—acetyl-CoA, NADPH, ATP—with above-mentioned products, as a reporter metabolite peroxisomal engineering in yeast Komagataella phaffii. To end, malonyl-CoA reductase pathway 3-HP production was targeted peroxisome K. phaffii using PTS1-tagging system, further tested different carbon sources. Thereafter, used compartmentalized system to showcase impact strategies aimed at enhancing NADPH pool. Co-overexpression genes encoding NADPH-dependent redox shuttle from Saccharomyces cerevisiae (IDP2/IDP3) significantly increased yields across all substrates, whereas targeting S. NADH kinase Pos5 failed improve production. This study highlights biosensor evaluating acetyl-CoA NAPDH availability by simply quantifying 3-HP, demonstrating its peroxisome-based metabolic yeast.

Язык: Английский

Процитировано

1
Efficient Production of the Colorless Carotenoid Phytoene in Yarrowia lipolytica through Metabolic Engineering DOI

Kwanghyun Park,

Gyeongmin Kim,

Sungman Cha

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 19, 2024

Phytoene, a colorless carotenoid with unique ultraviolet (UV)–B absorption properties, offers potential for applications in functional food, cosmetics, and therapeutics. However, their low natural yield poses challenge large-scale production. This study aims to enhance phytoene production the oleaginous yeast Yarrowia lipolytica by introducing heterologous synthase gene combined metabolic engineering approaches. We enhanced synthesis overexpressing key genes mevalonate pathway compartmentalizing biosynthetic within peroxisomes. Moreover, we inhibited glyoxylate cycle increase accumulation of peroxisomal acetyl-CoA available Our engineered strains demonstrated significant production, reaching up 1.34 g/L titer 58.74 mg/gDCW flask-scale fed-batch culture, which are highest levels reported date. These results underscore Y. as robust platform producing phytoenes other terpenoids on an industrial scale, offering valuable insights future efforts engineering.

Язык: Английский

Процитировано

0