Biotechnology Advances,
Год журнала:
2024,
Номер
unknown, С. 108492 - 108492
Опубликована: Дек. 1, 2024
The
prevalence
of
multidrug-resistant
(MDR)
ESKAPE
pathogens,
including
Enterococcus
faecium,
Staphylococcus
aureus,
Klebsiella
pneumoniae,
Acinetobacter
baumannii,
and
Pseudomonas
aeruginosa,
represents
a
critical
global
public
health
challenge.
In
response,
mRNA
vaccines
offer
an
adaptable
scalable
platform
for
immunotherapy
against
pathogens
by
encoding
specific
antigens
that
stimulate
B-cell-driven
antibody
production
CD8
Pharmaceuticals,
Год журнала:
2024,
Номер
17(9), С. 1206 - 1206
Опубликована: Сен. 12, 2024
The
rise
of
antibiotic
resistance
and
the
dwindling
antimicrobial
pipeline
have
emerged
as
significant
threats
to
public
health.
emergence
carbapenem-resistant
Annals of Medicine,
Год журнала:
2025,
Номер
57(1)
Опубликована: Янв. 10, 2025
Background
The
incidence
of
invasive
infection
Klebsiella
pneumoniae
(Kp)
in
the
community
is
increasing
every
year,
and
high
disability
mortality
rates
associated
with
them
pose
great
challenges
clinical
practice.
This
study
aimed
to
explore
microbiological
characteristics
Kp
community.
Vaccines,
Год журнала:
2025,
Номер
13(1), С. 59 - 59
Опубликована: Янв. 10, 2025
Background/Objectives
Bacterial
ghosts
(BGs),
non-living
empty
envelopes
of
bacteria,
are
produced
either
through
genetic
engineering
or
chemical
treatment
retaining
the
shape
their
parent
cells.
BGs
considered
vaccine
candidates,
promising
delivery
systems,
and
adjuvants.
The
practical
use
in
development
for
humans
is
limited
because
concerns
about
preservation
viable
bacteria
BGs.
Methods:
To
increase
efficiency
Klebsiella
pneumoniae
BG
formation
and,
accordingly,
to
ensure
maximum
killing
we
exploited
previously
designed
plasmids
with
lysis
gene
E
from
bacteriophage
φX174
holin–endolysin
systems
λ
L-413C
phages.
Previously,
this
kit
made
it
possible
generate
bacterial
cells
Yersinia
pestis
varying
degrees
hydrolysis
variable
protective
activity.
Results:
In
current
study,
showed
that
co-expression
holin
endolysin
genes
phage
elicited
more
rapid
efficient
K.
than
mediated
by
only
single
low
functioning
system
phage.
introduction
alternative
lysing
factors
into
instead
protein
leads
loss
murein
skeleton.
resulting
frameless
cell
envelops
reminiscent
sacs
skins
Although
such
structures
less
naive
classical
ghosts,
they
provide
effective
protection
against
infection
a
hypervirulent
strain
can
be
recommended
as
candidate
vaccines.
For
our
generated
using
O1:K2
strain,
both
safety
immunogenicity
aspects
were
evaluated.
Humoral
cellular
immune
responses
significantly
increased
mice
intraperitoneally
immunized
compared
subcutaneously
vaccinated
animals
(p
<
0.05).
Conclusions:
Therefore,
study
presents
novel
perspectives
future
research
on
ghost
Emerging Microbes & Infections,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 16, 2025
Phages
demonstrate
remarkable
promise
as
antimicrobial
agents
against
antibiotic-resistant
bacteria.
However,
the
emergence
of
phage-resistant
strains
poses
challenges
to
their
effective
application.
In
this
paper,
we
presented
isolation
a
phage
adaptive
mutant
that
demonstrated
enhanced
and
sustained
antibacterial
efficacy
through
co-evolution
Klebsiella
pneumoniae
(K.
pneumoniae)
111-2
ZX1Δint
in
vitro.
Our
experiments
revealed
successfully
completed
adsorption
phase
by
binding
host
surface,
specifically
targeting
capsular
polysaccharide
(CPS)
receptor
via
primary
receptor-binding
protein
(RBP)
ORF60
auxiliary
RBP
ORF59.
Upon
exposure
predation,
mutations
genes
wbaP,
wbaZ
or
wzc,
which
encode
synthesis
CPS,
conferred
resistance
reducing
adsorption.
response
these
defense
mechanisms,
phages
have
evolved
utilize
an
alternative
site
located
on
O-antigen
lipopolysaccharide
(LPS)
mutation
backup
This
evolutionary
change
enabled
reinfect
previously
strains.
Notably,
PR2
carrying
ORF59
Q777R,
capacity
infect
both
wild-type
resistant
strains,
exhibiting
prolonged
activity
wild
conclusion,
our
findings
elucidated
complex
phage-host
adsorption-antagonism
mechanism
characterized
mutation-driven
alterations
recognition.
work
contributes
deeper
understanding
adaptability
highlights
potential
for
combat
bacteria
vitro
approach.
Abstract
Klebsiella
pneumoniae
(KP),
recognized
for
its
pronounced
antibiotic
resistance,
is
a
prevalent
agent
of
nosocomial
infections
such
as
hospital‐acquired
pneumonia.
The
alarming
rate
serious
and
associated
mortality
renders
KP
significant
public
health
threat.
Extracellular
vesicles
(EVs)
are
pivotal
in
KP's
physiological
pathological
mechanisms,
facilitating
material
information
transfer
mediating
interactions
between
the
bacteria
host.
critical
role
EVs
enhances
our
understanding
pathophysiology,
disease
progression,
strategies
infection
control.
This
review
emphasizes
mechanics
resistance
instrumental
bacterium‐host
interplay,
proposing
promising
research
focus
advancing
diagnosis,
therapy,
prevention.
PLoS Pathogens,
Год журнала:
2025,
Номер
21(4), С. e1012971 - e1012971
Опубликована: Апрель 8, 2025
Klebsiella
pneumoniae
(KP)
is
a
Gram-negative
bacterium
that
commonly
resides
in
the
human
gastrointestinal
tract
and
can
also
act
as
an
opportunistic
pathogen
cause
extra-intestinal
infections.
KP
poses
global
health
threat
because
it
causes
both
hospital-
community-acquired
infections
immune-competent
immunocompromised
hosts.
These
be
multidrug-resistant
and/or
hypervirulent,
making
difficult
to
treat
deadly.
In
absence
of
effective
treatments
for
recalcitrant
infections,
bacteriophage
(phage)
therapy
gaining
attention
promising
alternative.
this
review,
we
evaluate
epidemiology
epitope
diversity,
discuss
interactions
between
KP-targeting
phages
their
bacterial
hosts
from
eco-evolutionary
perspective,
summarize
recent
efforts
phage
treating
We
novel
approaches,
including
genetic
engineering
machine
learning,
initial
steps
toward
developing
precision
medicine
approach
emerging
dangerous
pathogen.
