Antibiotics, Год журнала: 2024, Номер 13(11), С. 1045 - 1045
Опубликована: Ноя. 5, 2024
Antimicrobial resistance represents a challenge to public health systems because of the array and virulence mechanisms that lead treatment failure increased mortality rates. Although for years main driver carbapenem in Italy has been
Язык: Английский
Microbiological Research, Год журнала: 2025, Номер 293, С. 128049 - 128049
Опубликована: Янв. 5, 2025
Язык: Английский
Процитировано
1
Pathogens and Immunity, Год журнала: 2025, Номер 10(1), С. 80 - 119
Опубликована: Янв. 24, 2025
Klebsiella pneumoniae (Kp) is a Gram-negative pathogen responsible for both hospital- and community-acquired infections. Kp classified into 2 distinct pathotypes: classical K. (cKp) hypervirulent (hvKp). First described in Taiwan 1986, hvKp are highly pathogenic characterized by unique phenotypic genotypic traits. The hypermucoviscous (hmv) phenotype, generally marked overproduction of the capsule, often associated with hvKp, although recent studies show that some cKp strains may also have this characteristic. Furthermore, can cause severe infections healthy people been metastatic such as liver abscess, meningitis, endophthalmitis. HvKp increasingly being reported hospital-acquired settings, complicating treatment strategies. In particular, while historically antibiotic-susceptible, multidrug-resistant (MDR) emerged pose significant public health threat. combination high virulence limited antibiotic options demands further research mechanisms rapid identification methods. This review discusses epidemiology their factors, highlighting importance non-phenotypic tests, including next-generation molecular diagnostics, early detection hvKp.
Язык: Английский
Процитировано
0
Frontiers in Medicine, Год журнала: 2025, Номер 12
Опубликована: Фев. 5, 2025
To compare the risk factors, clinical outcomes, and mortality rates between carbapenem-resistant Klebsiella pneumoniae (CRKP) carbapenem-sensitive (CSKP) infections. A retrospective cohort study was conducted on patients with infections admitted to a tertiary hospital in Zhangjiakou, China. The research period is from January 2021 December 2022. Data were analyzed using SPSS 24.0 R. Univariate analysis identified potential factors for CRKP appropriate statistical methods, followed by multivariable logistic regression determine independent factors. Mortality CSKP groups compared chi-square tests, survival curves generated Kaplan-Meier method. included 283 patients, 104 (36.7%) infected 179 (63.3%) CSKP. had significantly higher body temperature, white blood cell counts, inflammatory markers, while showing lower diastolic pressure oxygen saturation (p < 0.05). predominantly found ICU (49%) mainly isolated sputum (59%). Independent elevated C-reactive protein (OR = 1.02) solid tumors 18.186). experienced longer stays (25 days vs. 12 CSKP), (13 7 days), 30-day (23.1% 17.9%, p 0.012). deceased group procalcitonin creatinine levels, prothrombin time, greater need mechanical ventilation survivors Patients than those Previous hospitalization, hospitalization an ICU, infection.
Язык: Английский
Процитировано
0
Frontiers in Microbiology, Год журнала: 2025, Номер 16
Опубликована: Фев. 24, 2025
Klebsiella pneumoniae is an opportunistic pathogen primarily associated with nosocomial infections, characterized by a propensity for multi-drug resistance and the potential evolution into hypervirulent strains. Based on its phenotypic genotypic characteristics, K. can be classified two types: classical (cKP) (hvKP). The spread of mobile genetic elements (MGEs) in has led to emergence carbapenem-resistant (CRKP) (CR-hvKP). CR-hvKP particularly concerning due multidrug resistance, high pathogenicity, increased transmissibility. This review summarizes types MGEs present pneumoniae, mechanisms horizontal gene transfer (HGT) mediated these elements, their roles dissemination antibiotic genes (ARGs) virulence genes, relationships among that resemble Russian dolls or exhibit hybrid characteristics. Additionally, clinical treatment epidemiological characteristics are discussed. Given variability transmissibility MGEs, continuous monitoring control variation transmission such material should prioritized.
Язык: Английский
Процитировано
0
Journal of Microbiology Immunology and Infection, Год журнала: 2025, Номер unknown
Опубликована: Фев. 1, 2025
KL1, KL2 CR-hvKP and KL64, KL47 hv-CRKP all exhibit overlapping multidrug resistance hypervirulence phenotypes, but the differences in epidemiological, phenotypic genotypic characteristics between them remains unclear. In this study, we collected non-repeated hv-CRKP/CR-hvKP isolates a tertiary hospital Shanghai, China from January 2019 to December 2022. Furthermore, selected four typical hypervirulent carbapenem-resistant Klebsiella pneumoniae, including ST23-KL1/ST86-KL2 (WYKP3 WYKP194) ST11-KL64/ST11-KL47 (WYKP589 WYKP188), tried clarify compare their virulence drug plasmid distribution. Our study found that ST23-KL1 ST86-KL2 exhibited less diversity than of ST11-KL64 ST11-KL47 hv-CRKP. Compared with hv-CRKP, harbored significantly fewer antimicrobial genes more genes, which contributed higher these strains antibiotics. has emerged as most prevalent pneumoniae probably due its clonal transmission within hospitals well plasmids help conjugative plasmids. Due different evolutionary mechanisms capsular serotypes, are different.
Язык: Английский
Процитировано
0
Next research., Год журнала: 2025, Номер unknown, С. 100276 - 100276
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Journal of Bacteriology, Год журнала: 2025, Номер unknown
Опубликована: Март 26, 2025
ABSTRACT Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a clinical pathogen with high mortality rate, and its management infection control have become serious challenge. Phage-encoded depolymerase cleaves the capsular polysaccharide, major virulence factor of K. . This study aimed to identify phage targeting ST11 K64 CRKP, evaluate antimicrobial activity therapeutic efficacy, provide new alternative strategies for CRKP. Phages were screened from untreated hospital sewage using clinically isolated CRKP as host bacterium. The range, efficiency plaque formation, optimal multiplicity infection, adsorption efficiency, one-step growth curve vB_KpnP_IME1309 determined by double-layer agar plate culture method. morphology was observed transmission electron microscopy. Phage nucleic acids extracted whole-genome sequencing, phage-encoded gene ORF37 amplified polymerase chain reaction. Next, recombinant plasmid constructed induce expression, which verified sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In vitro bactericidal combined serum assay, anti- K biofilm effect crystal violet staining. Finally, Galleria mellonella larvae model established investigate on in vivo Here, we characterized featured latent period 20 min burst size approximately 290 plaque-forming units/cell. It contained 41 predicted open reading frames, encoded depolymerase. expressed purified Dep37 cleaved only formed translucent halo plate. increased susceptibility B1 killing, inhibited degraded mature biofilms. combination kanamycin significantly more effective treating biofilms compared either or alone. An injection at 5 2 h after their survival rates up 73% 53%, respectively. Depolymerase may be used potential method capsule typing , showing great promise development novel against IMPORTANCE A carbapenem-resistant characterized. encoding successfully purified. increases inhibits degrades than alone treatment biofilm. rate
Язык: Английский
Процитировано
0
Folia Microbiologica, Год журнала: 2025, Номер unknown
Опубликована: Апрель 8, 2025
Язык: Английский
Процитировано
0
Cureus, Год журнала: 2025, Номер unknown
Опубликована: Апрель 8, 2025
Язык: Английский
Процитировано
0
Journal of Infection, Год журнала: 2025, Номер 90(6), С. 106493 - 106493
Опубликована: Апрель 24, 2025
Язык: Английский
Процитировано
0