Comparative Genomics of Bifidobacterium animalis subsp. lactis Reveals Strain-Level Hyperdiversity, Carbohydrate Metabolism Adaptations, and CRISPR-Mediated Phage Immunity DOI Creative Commons
Özge Can, Ismail Gumustop,

Ibrahim Genel

и другие.

Fermentation, Год журнала: 2025, Номер 11(4), С. 179 - 179

Опубликована: Март 31, 2025

Several strains of Bifidobacterium animalis subsp. lactis are blockbusters commercial dietary supplement cocktails, widely recognized for their probiotic properties and found in various ecological niches. The present study aimed to perform an in-depth comparative genomic analysis on 71 B. isolated from diverse sources, including human animal feces, breast milk, fermented foods, supplements, better elucidate the strain level diversity biotechnological potential this species. average genome size was be 1.93 ± 0.05 Mb, with a GC content 60.45% 0.2, 1562 41.3 coding sequences (CDS), 53.4 1.6 tRNA genes. A revealed significant genetic among strains, core showing that 34.7% total genes were conserved, while pan-genome remained open, indicating ongoing gene acquisition. Functional annotation through EggNOG-Mapper CAZYme clustering highlighted metabolic capabilities, particularly carbohydrate metabolism. Nearly all (70 71) harbor CRISPR-Cas adaptive immune systems (predominantly Type I-E subtype), underscoring ubiquity phage defense mechanism spacer distinct strain-specific CRISPR profiles, certain sharing identical spacers correlate common phylogenetic clades or similar isolation sources—an indication exposure same populations shared selective pressures. These findings highlight dynamic co-evolution between its bacteriophages across niches point leveraging native future applications. Our enhance our understanding functional lactis, providing valuable insights use probiotics foods.

Язык: Английский

Where Biology Meets Engineering: Scaling Up Microbial Nutraceuticals to Bridge Nutrition, Therapeutics, and Global Impact DOI Creative Commons
Ahmed M. Elazzazy, Mohammed N. Baeshen,

Khalid M. Alasmi

и другие.

Microorganisms, Год журнала: 2025, Номер 13(3), С. 566 - 566

Опубликована: Март 2, 2025

The global nutraceutical industry is experiencing a paradigm shift, driven by an increasing demand for functional foods and dietary supplements that address malnutrition chronic diseases such as obesity, diabetes, cardiovascular conditions, cancer. Traditional plant- animal-derived nutraceuticals face limitations in scalability, cost, environmental impact, paving the way microbial biotechnology sustainable alternative. Microbial cells act bio-factories, converting nutrients like glucose amino acids into valuable products polyunsaturated fatty (PUFAs), peptides, other bioactive compounds. By harnessing their natural metabolic capabilities, microorganisms efficiently synthesize these compounds, making production effective approach development. This review explores transformative role of platforms nutraceuticals, emphasizing advanced fermentation techniques, synthetic biology, engineering. It addresses challenges optimizing strains, ensuring product quality, scaling while navigating regulatory frameworks. Furthermore, highlights cutting-edge technologies CRISPR/Cas9 genome editing, adaptive evolution strain enhancement, bioreactor innovations to enhance yield efficiency. With focus on sustainability precision, positioned game-changer industry, offering eco-friendly scalable solutions meet health needs. integration omics exploration novel sources hold potential revolutionize this field, aligning with growing consumer innovative products.

Язык: Английский

Процитировано

2
Comparative Genomics of Bifidobacterium animalis subsp. lactis Reveals Strain-Level Hyperdiversity, Carbohydrate Metabolism Adaptations, and CRISPR-Mediated Phage Immunity DOI Creative Commons
Özge Can, Ismail Gumustop,

Ibrahim Genel

и другие.

Fermentation, Год журнала: 2025, Номер 11(4), С. 179 - 179

Опубликована: Март 31, 2025

Several strains of Bifidobacterium animalis subsp. lactis are blockbusters commercial dietary supplement cocktails, widely recognized for their probiotic properties and found in various ecological niches. The present study aimed to perform an in-depth comparative genomic analysis on 71 B. isolated from diverse sources, including human animal feces, breast milk, fermented foods, supplements, better elucidate the strain level diversity biotechnological potential this species. average genome size was be 1.93 ± 0.05 Mb, with a GC content 60.45% 0.2, 1562 41.3 coding sequences (CDS), 53.4 1.6 tRNA genes. A revealed significant genetic among strains, core showing that 34.7% total genes were conserved, while pan-genome remained open, indicating ongoing gene acquisition. Functional annotation through EggNOG-Mapper CAZYme clustering highlighted metabolic capabilities, particularly carbohydrate metabolism. Nearly all (70 71) harbor CRISPR-Cas adaptive immune systems (predominantly Type I-E subtype), underscoring ubiquity phage defense mechanism spacer distinct strain-specific CRISPR profiles, certain sharing identical spacers correlate common phylogenetic clades or similar isolation sources—an indication exposure same populations shared selective pressures. These findings highlight dynamic co-evolution between its bacteriophages across niches point leveraging native future applications. Our enhance our understanding functional lactis, providing valuable insights use probiotics foods.

Язык: Английский

Процитировано

0