Ferroptosis-Related Genes as Molecular Markers in Bovine Mammary Epithelial Cells Challenged with Staphylococcus aureus

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(6), С. 2506 - 2506

Опубликована: Март 11, 2025

Staphylococcus aureus-induced mastitis is a significant cause of economic losses in the dairy industry, yet its molecular mechanisms remain poorly defined. Although ferroptosis, regulated cell death process, associated with inflammatory diseases, role bovine unknown. In this study, 11 S. aureus strains were isolated from milk samples obtained cows clinical or subclinical mastitis. Transcriptome analysis Mac-T cells challenged identified differentially expressed genes (DEGs). Enrichment revealed associations between DEG clusters and traits related to KEGG pathway enrichment Toll-like receptor, TNF signaling as significantly enriched pathways. Weighted gene co-expression network (WGCNA) further prioritized ferroptosis-related (HMOX1, SLC11A2, STEAP3, SAT1, VDAC2) involved iron metabolism. Notably, expression levels HMOX1 SAT1 increased aureus-challenged cells, upregulation was consistent trends observed transcriptome data mother–daughter pairs caused by infection. Furthermore, Ferrostatin-1 treatment reduced confirming involvement ferroptosis process. This study reveals that plays key highlights potential target for breeding strategies aimed at improving resistance.

Язык: Английский

Photocatalytic TiO₂/HAP nanocomposite for antimicrobial treatment, promineralization, and tooth whitening

RSC Advances, Год журнала: 2025, Номер 15(17), С. 13453 - 13467

Опубликована: Янв. 1, 2025

Illustration of the mechanism TiO 2 -HAP nanocomposite with multifunctional properties for potential application in early caries.

Язык: Английский