Therapeutic Potential of Endophytic Microbes: Emphasizing Both Fungal and Bacterial Endophytes
Applied Microbiology,
Год журнала:
2025,
Номер
5(1), С. 5 - 5
Опубликована: Янв. 5, 2025
This
review
explores
the
diverse
applications
and
therapeutic
potential
of
endophytic
microbes,
emphasizing
both
fungal
bacterial
endophytes.
These
microorganisms
reside
within
plant
tissues
without
causing
harm
play
an
important
role
in
enhancing
growth,
nutrient
acquisition,
resistance
to
pathogens.
They
produce
phytohormones,
facilitate
uptake,
solubilize
essential
nutrients,
fix
nitrogen,
improve
stress
tolerance.
Furthermore,
endophytes
contribute
agricultural
sustainability
by
producing
growth
regulators,
providing
biocontrol
against
pathogens
through
antimicrobial
compounds,
competing
for
resources.
Integrating
microbes
into
practices
can
reduce
reliance
on
chemical
fertilizers
pesticides,
promoting
eco-friendly
sustainable
farming.
highlights
dual
fostering
agriculture
novel
applications.
By
minimizing
dependence
inputs,
support
environmental
health
while
boosting
crop
yields.
The
synthesis
underscores
importance
leveraging
tackle
global
food
security
challenges.
Язык: Английский
Unveiling microbial complexity within Astragalus propinquus and Glycyrrhiza uralensis roots
Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 17, 2025
Abstract
Background
Astragalus
propinquus
(
AP)
and
Glycyrrhiza
uralensis
(
GU),
members
of
the
Fabaceae
family,
are
widely
used
for
their
therapeutic
properties.
However,
endophytic
microbial
communities
in
roots
remain
largely
unknown.
Herein,
we
compared
structure
properties
root-associated
bacterial
fungal
AP
GU,
specifically
excluding
thriving
rhizosphere,
using
both
culture-dependent
-independent
methods.
Results
A
metabarcoding-based
approach
revealed
a
higher
abundance
Proteobacteria
root
microbiome
GU
than
that
AP.
Fungal
showed
similar
distinctions,
with
predominantly
harboring
Ascomycota
Basidiomycota,
respectively.
The
community
exhibited
significantly
diversity
included
unique
taxa,
e.g.,
Steroidobacterales
Micromonosporales.
was
relatively
less
diverse
dominated
by
Xanthomonadales.
Differential
analysis
plant
species
impacted
301
228
amplicon
sequence
variants
(ASVs)
GU.
Among
these,
B5_f_
Comamonadaceae
markedly
more
enriched
random
forest
model
analyzing
ASVs
significant
differences
indicated
most
were
pan-microbial
1,243
identified,
including
96
co-detected
between
3
core
(B2_f_
Pseudomonas,
B5_
Comamonadaceae,
B70_
Cutibacterium).
comprised
435
ASVs,
98
shared
8
(F5_
Paraphoma,
F6_f_
Lysurus,
F22_
Alternaria,
F30_
Phaeosphaeria,
F53_
Cladosporium,
F36_
Moesziomyces,
F55_f_
Neocucurbitaria,
F56_
Malassezia).
Hub
nodes
identified
to
elucidate
roles
microorganisms
within
networks.
In
AP,
B152_o_
Burkholderiales,
F14_
Exophiala,
F33_
Fusarium
key
hub
nodes,
whereas,
B36_
Paenibacillus
central
node.
comparative
analyses
in
vitro
culture
data
molecular
sequencing
results
overlapping
patterns,
Pseudomonas
dominant
Bacillus
Conclusions
These
findings
highlight
distinct
each
exhibiting
orders
network
complexity
diversity.
suggest
potential
functional
contributions,
nutrient
cycling
secondary
metabolite
production,
communities,
likely
impacting
these
plants.
Язык: Английский
Exploring the Antimicrobial, Antioxidant and Extracellular Enzymatic Activities of Culturable Endophytic Fungi Isolated from the Leaves of Kirkia acuminata Oliv
Microorganisms,
Год журнала:
2025,
Номер
13(3), С. 692 - 692
Опубликована: Март 19, 2025
Fungal
endophytes
of
medicinal
plants
produce
diverse
secondary
metabolites
and
extracellular
enzymes
with
therapeutic
biotechnological
potential.
However,
the
biological
potential
fungal
from
South
African
remain
relatively
underexplored.
In
this
study,
antimicrobial,
antioxidant,
anti-inflammatory
enzymatic
capabilities
five
previously
isolated
leaves
Kirkia
acuminata
Oliv.
were
investigated.
Sequencing
internal
transcribed
spacer
(ITS)
regions
revealed
that
isolates
belonged
to
genera
Setosphaeria,
Diaporthe
Corynespora.
The
broth
micro-dilution
assay
Folin–Ciocalteau
reagent
method
used
assess
antibacterial
activity
total
phenolic
content
(TPC)
endophytes’
ethyl
acetate
crude
extracts
(CEs),
respectively.
antioxidant
was
assessed
using
ferric
reducing
power
(FRAP)
1,1-diphenyl-2-picrylhydrazyl
(DPPH)
free
radical
scavenging
assays.
influence
CE
Setosphaeria
rostrata
KaL-4
on
viability
LPS-induced
interleukin-6
(IL-6)
production
in
Raw
264.7
macrophages
a
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide
(MTT)
an
ELISA,
ability
proteases,
laccases
peroxidases
also
determined.
CEs
displayed
antimicrobial
MICs
ranging
0.63
1.25
mg/mL
40%
18%
60%
48%,
S.
possessed
highest
TPC
demonstrated
dose-dependent
cytotoxicity.
further
significant
reduction
IL-6
at
concentration
0.75
µg/mL.
Only
one
isolate
proteases
index
(EI)
0.66,
while
(EI
range
0.14
1.15)
produced
by
all
isolates.
These
findings
suggest
are
promising
sources
bioactive
compounds
industry-significant
enzymes.
Язык: Английский
Mechanistic understanding of metabolic cross-talk between Aloe vera and native soil bacteria for growth promotion and secondary metabolites accumulation
Frontiers in Plant Science,
Год журнала:
2025,
Номер
16
Опубликована: Март 27, 2025
Plants
release
a
wealth
of
metabolites
into
the
rhizosphere
that
can
influence
composition
and
activity
microbial
communities.
These
communities,
in
turn,
affect
growth
metabolism
host
plant.
The
connection
between
medicinal
plant
its
associated
microbes
has
been
suggested,
yet
mechanisms
underlying
selection
indigenous
microbes,
their
biological
function
plants
are
largely
unknown.
In
this
study,
we
investigated
how
Aloe
vera
select
bacteria
examined
functional
roles
relation
to
benefit.
We
utilized
two
native
promoting
rhizobacterial
(PGPR)
strains
:
Paenibacillus
sp.
GLAU-BT2
Arthrobacter
GLAU-BT16,
as
either
single
or
consortium
inoculants
for
experiment.
analyzed
non-targeted
root
presence
both
bacterial
confirmed
exudation
rhizosphere.
GC-MS
analysis
revealed
inoculation
amplified
abundance
flavonoids,
terpenes
glucoside
roots,
which
also
exuded
Flavonoids
were
most
common
prevalent
metabolite
group
individual
inoculants,
highlighting
role
key
interactions
with
microbes.
addition,
significantly
increased
antioxidant
well
total
phenolic
flavonoid
content
leaves
.
conclusion,
propose
model
circular
metabolic
communication
induce
production
flavonoids
plants.
releases
some
these
support
community
own
Язык: Английский