Analytical Sciences,
Год журнала:
2023,
Номер
39(9), С. 1551 - 1560
Опубликована: Май 28, 2023
The
sensitive,
non-destructive
constant
wavelength
(CW)
and
energy
(CE)
SFS
techniques
have
been
used
for
the
simultaneous
determination
of
1-amino
pyrene
(AP)
1-napthyl
amine
(NA)
in
their
mixtures
without
prior
separation
via
optimization
different
experimental
conditions
(ΔλCW
70.0
nm,
ΔνCE
4000.0
cm−1,
scan
rate
240.0
nm/min,
25.0
°C,
methanol).
Amplitude–concentration
plots
linear
pyrene,
AP
(0.01–0.1
mg/L)
amine,
NA
(0.1–1.0
mg/L).
In
aqueous
methanolic
binary
mixtures,
mean
recoveries
(RSD,
LOD
LOQ)
were
found
to
be
100.09%
(0.053,
0.008
mg/L
0.034
emission,
100.11%
(0.141,
mg/L,
CWSFS,
100.05%
(0.109,
0.007
0.032
first
derivative
100.00%
(0.148,
0.031
CESFS,
99.99%
0.035
CESFS
modes
respectively.
Additionally,
100.29%
(0.360,
0.046
0.204
100.06%
(0.089,
0.098
0.436
(0.144,
0.065
0.288
(0.178,
0.077
0.339
100.03%
(0.181,
0.082
0.364
Considering
safety
greenness,
these
methods
might
considered
as
green
tools
using
analytical
eco-scale
approaches
(eco-scale
score
88.0).
Geomicrobiology Journal,
Год журнала:
2023,
Номер
41(4), С. 404 - 416
Опубликована: Июнь 5, 2023
AbstractAbstractPolycyclic
aromatic
hydrocarbons
(PAHs)
are
persistent
organic
pollutants
that
impart
several
hazardous
effects
on
the
environment.
Extracellular
enzymes
released
by
filamentous
fungi
well-known
participants
in
degradation
of
such
pollutants.
The
present
study
aims
to
determine
interaction
pyrene
with
Lac1
protein,
coding
for
extracellular
laccase
enzyme
from
Trichoderma
sp.
CNSC-2.
sequence
analysis
revealed
stable
and
acidic
nature
enzyme.
dominance
random
coils
(54.8%)
extended
strands
(28.3%)
secondary
structure
defined
protein
flexibility.
3D
homology
modeling
followed
validation
a
high-quality
model
94.8%
residues
favored
zone
Ramachandran
plot.
Molecular
docking
displayed
significant
binding
affinity
−7.82
kcal
mol−1
amino
acid
at
active
site
Lac1,
indicating
strong
role
binding.
CNSC-2
achieved
69.42
±
0.4%
removal
efficiency
100
mg
l−1
concentration
day
12
incubation.
Laccase
activity
during
simultaneously
increased,
highest
was
observed
6
Enzyme
production
found
be
optimum
pH
(p
<
0.001)
30°C
temperature
0.0001).
FTIR
showed
deviation
amide
bands
presence
pyrene,
additional
peaks
coinciding
metabolites
were
observed.
decrease
intensity
due
quenching
determined
fluorescence
spectroscopy.
Thus,
this
suggested
implying
potential
degradation.Keywords:
Binding
affinitydockinghomology
modelinglaccasepyreneTrichoderma
AcknowledgementsThe
authors
would
like
acknowledge
authorities
NIT
Rourkela,
Odisha
providing
necessary
facilities.Disclosure
statementNo
conflict
interest
reported
author(s).Authors'
contributionsADB
NC
conducted
experiments.
ADB
acquired
analyzed
data.
SK
drafted
original
manuscript.
SD
designed
research,
secured
fund,
interpreted
data
revised
All
read
agreed
final
manuscript.Data
availability
statementAll
generated
or
have
been
included
manuscript
supplementary
material.
sequencing
raw
deposited
NCBI
Sequence
Read
Archive
database
under
accession
number
OP858805
(Lac1).Additional
informationFundingAuthors
thank
Department
Biotechnology,
Government
India,
financial
assistance
[BT/PR9465/NDB/39/360/2013].
is
grateful
Council
Scientific
Industrial
Research,
India
[09/983(0048)/2020-EMR-I]
fellowship
doctoral
study.
Journal of Mines Metals and Fuels,
Год журнала:
2024,
Номер
unknown, С. 399 - 406
Опубликована: Дек. 20, 2024
The
present
review
study
manifests
the
sampling
techniques,
analytical
tools
and
status
of
Polycyclic
Aromatic
Hydrocarbons
(PAHs)
in
different
environmental
samples
Indian
urban
cities.
Indeed,
petrogenic,
pyrogenic
biogenic
sources
PAHs
grounds
to
carcinogenic,
mutagenic,
teratogenic
genotoxic
effects
on
human
environment.
Most
studies
focussed
ambient
air
noticed
that
carcinogenic
Benzo[a]
Pyrene
is
dominant
severe
health
risk
posturing
4-5
ring
high
molecular
weight
PAHs.
High-volume
sampler
for
minimum
8hrs
espoused
as
best
method
over
passive
bio
samplers,
glass
fibre
filters,
particulate
samplers
etc.
microwave
assisted
Soxhlet
extraction
with
n-hexane
solvent
has
gained
a
lot
attention
due
its
pragmatism
sample
handling
than
ultrasonic.
high-performance
liquid
chromatography
gas
mass
spectrophotometer
adopted
accuracy.
concentration
among
11
cities
significantly
emphasized
inter
intra
spatial
variations.
Moreover,
amount
differs
air,
water
soil.
Also,
comparative
revealed
Delhi
Amravati
recorded
maximum
concentrations
respectively.
Analytical Sciences,
Год журнала:
2023,
Номер
39(9), С. 1551 - 1560
Опубликована: Май 28, 2023
The
sensitive,
non-destructive
constant
wavelength
(CW)
and
energy
(CE)
SFS
techniques
have
been
used
for
the
simultaneous
determination
of
1-amino
pyrene
(AP)
1-napthyl
amine
(NA)
in
their
mixtures
without
prior
separation
via
optimization
different
experimental
conditions
(ΔλCW
70.0
nm,
ΔνCE
4000.0
cm−1,
scan
rate
240.0
nm/min,
25.0
°C,
methanol).
Amplitude–concentration
plots
linear
pyrene,
AP
(0.01–0.1
mg/L)
amine,
NA
(0.1–1.0
mg/L).
In
aqueous
methanolic
binary
mixtures,
mean
recoveries
(RSD,
LOD
LOQ)
were
found
to
be
100.09%
(0.053,
0.008
mg/L
0.034
emission,
100.11%
(0.141,
mg/L,
CWSFS,
100.05%
(0.109,
0.007
0.032
first
derivative
100.00%
(0.148,
0.031
CESFS,
99.99%
0.035
CESFS
modes
respectively.
Additionally,
100.29%
(0.360,
0.046
0.204
100.06%
(0.089,
0.098
0.436
(0.144,
0.065
0.288
(0.178,
0.077
0.339
100.03%
(0.181,
0.082
0.364
Considering
safety
greenness,
these
methods
might
considered
as
green
tools
using
analytical
eco-scale
approaches
(eco-scale
score
88.0).
