Simultaneous Quantification of 66 Compounds in Two Tibetan Codonopsis Species Reveals Four Chemical Features by Database-Enabled UHPLC-Q-Orbitrap-MS/MS Analysis

Molecules, Год журнала: 2024, Номер 29(21), С. 5203 - 5203

Опубликована: Ноя. 3, 2024

Codonopsis canescens Nannf. (CoC) and nervosa (CoN) are two traditional Tibetan medicinal herbs (Zangdangshen), which have been widely used in the treatment of various diseases. In this study, their aerial underground parts were systematically analyzed using database-enabled UHPLC-Q-Orbitrap-MS/MS technology. This technology introduced three adduct ions, [M − H]−, + H]+, NH4]+, to putatively identify a total 66 compounds. During putative identification, at least 16 isomers successfully differentiated, such as isochlorogenic acid A vs. B C. Thereafter, all these identified compounds further quantified for contents based on linear regression method. Their observed vary from 0.00 39,127.03 µg/g. Through multiple comparisons quantification results, study found following four chemical features: (1) Four sesquiterpenes (especially atractylenolide III) enriched mainly CoC rarely CoN; (2) quinic derivatives abundant part species; (3) sixteen flavonoids (particularly diosmetin chrysoeriol) showed higher content than (4) lobetyolin was ubiquitously distributed both CoN. Based features relevant principles, (lobetyolin, III, diosmetin, recommended quality markers species. All findings can facilitate sustainable development control herbs.

Язык: Английский

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Unlocking the Mysteries of the Desorption–Ionization Mechanism via Separate Thermal and Charge Strategies

Analytical Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 20, 2024

Herein, a new strategy is employed to build controllable thermal-coupled charge ionization (TCCI) device elucidate the desorption-ionization mechanism of plasma ion sources. Efficient synergistic desorption and are achieved within TCCI by independently controlling temperature charges. The efficiently ionizes samples using abundant free electrons, charges, active species from arc plasma. coexistence electrons hydroxide radicals confers redox capability system, implying presence unified even when transmitted through metal conductor over distance. In addition, molecular ions analytes facilitate differentiation between primary secondary amines during their analysis. Notably, enables switch hard soft adjusting thermal temperature. At high temperatures (>400 °C), exhibits characteristics, producing fragment beneficial for isomer discrimination. mass spectrometry robust performance in terms sensitivity accuracy detecting antibiotics sterols saline solutions, achieving linearity with correlation coefficients ≥0.99 excellent reproducibility. successful analysis seven pharmaceuticals four complex matrices demonstrates its salt matrix tolerance. Overall, device, independent control plasma, achieves efficient ionization, overcoming limitations existing technologies contributing study gas-phase dynamics mechanisms.

Язык: Английский

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Putative Identification of 12 Isomeric and 21 Non-isomeric Compounds from Leaves of Rubus Alceifolius Poir Using Database-affinity UHPLC-Q-Exactive-Qrbitrap-MS/MS

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 14, 2023

Abstract This is the first article to report of chemical profile leaves Rubus alceifolius Poir, a widely distributed medicinal plant in Eastern Hemisphere. Fresh R. were treated by set experimental protocols prepare lyophilized aqueous extract. A novel strategy was used analyze extract, i.e., The extract then analyzed using new strategy, database-affinity ultra-high-performance liquid chromatography-quadrupole-Exactive-Orbitrap-tandem mass spectrometry (UHPLC-Q-Exactive-Orbitrap-MS/MS). Using MS/MS at full elucidation and comparison with database, 33 compounds putatively identified, including 12 flavonoid derivatives, 6 phenolic acid caffeoylquinic acids, two tea polyphenols, others. Especially, isomers have been strictly distinguished, apigenin vs 2'-hydroxydaidzein, luteolin 7-O-glucuronide scutellarin, (+) catechin (-) epicatechin, 3 -O- 4 5 acid, 3,4 dicaffeoylquinic 3,5 4,5- O -dicaffeoylquinic acid. In addition, 21 non-isomeric also found under both negative positive ion models, such as ellagic gallic Structural suggested that there biogenetic relationships among glycosidation hydroxylation. All these findings will help understand substance basis traditional functions Alceifolius . structural between These allow scientific community better functions.

Язык: Английский

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