Revista Brasileira de Engenharia de Biossistemas, Год журнала: 2024, Номер 18

Опубликована: Дек. 23, 2024

With the increasing global demand for food, new pathways have emerged to drive development and manipulation of crops with desired traits ensure food security. CRISPR technology has enabled genome editing, allowing addition or destruction specific DNA sequences modify a function, stands out as an exceptionally versatile tool. The components system include Cas9 enzyme, responsible double-strand cuts, guide RNA (gRNA), forming part spacer RNA. Through systematic review, we searched, identifying 30 articles related technique its application in agriculture. CRISPR-Cas9 been widely employed understand transcriptional regulation, make epigenetic modifications, microscopically visualize loci. results support specificity editing CRISPR/Cas9 system, demonstrating efficiency enhancing crop performance, enabling generation plants free foreign DNA, avoiding off-target mutations. Overall, increased productivity, water stress resistance, weed control various evaluated. It plays pivotal role boosting agricultural creation adapted adverse environments, significantly thus represents fundamental tool genetic engineering, propelling significant innovations address challenges.

Язык: Английский