Silicon-based bimetallic nanozyme-enhanced immunochromatographic strips for highly sensitive simultaneous detection of multiple environmental pollutants
Chemical Engineering Journal,
Год журнала:
2025,
Номер
unknown, С. 159936 - 159936
Опубликована: Янв. 1, 2025
Язык: Английский
Lateral flow biosensor development for the visual identification of H1N1 virus based on primer extension nucleic acid isothermal amplification and M13mp18 single-stranded DNA
Microchimica Acta,
Год журнала:
2025,
Номер
192(3)
Опубликована: Фев. 18, 2025
Язык: Английский
Ultrasensitive Lateral Flow Immunoassay of Fluoroquinolone Antibiotic Gatifloxacin Using Au@Ag Nanoparticles as a Signal-Enhancing Label
Biosensors,
Год журнала:
2024,
Номер
14(12), С. 598 - 598
Опубликована: Дек. 6, 2024
Gatifloxacin
(GAT),
an
antibiotic
belonging
to
the
fluoroquinolone
(FQ)
class,
is
a
toxicant
that
may
contaminate
food
products.
In
this
study,
method
of
ultrasensitive
immunochromatographic
detection
GAT
was
developed
for
first
time.
An
indirect
format
lateral
flow
immunoassay
(LFIA)
performed.
GAT-specific
monoclonal
antibodies
and
labeled
anti-species
were
used
in
LFIA.
Bimetallic
core@shell
Au@Ag
nanoparticles
(Au@Ag
NPs)
synthesized
as
new
label.
Peroxidase-mimic
properties
NPs
allowed
catalytic
enhancement
signal
on
test
strips,
increasing
assay
sensitivity.
A
mechanism
NPs-mediated
catalysis
deduced.
Signal
amplification
achieved
through
oxidative
etching
by
hydrogen
peroxide.
This
resulted
formation
gold
Ag+
ions,
which
catalyzed
oxidation
peroxidase
substrate.
Such
“chemical
enhancement”
reaching
instrumental
limit
(LOD,
calculated
Three
Sigma
approach)
cutoff
0.8
20
pg/mL,
respectively.
The
enhanced
procedure
can
be
completed
21
min.
LFIA
tested
raw
meat
samples,
recoveries
from
78.1–114.8%.
recommended
promising
instrument
sensitive
various
toxicants.
Язык: Английский
The Transformative Role of Nanoenzymes in the Diagnosis, Targeted Treatment, and Prognosis of Ovarian Cancer. A comprehensive review
Developmental medico-life-sciences,
Год журнала:
2024,
Номер
1(10), С. 4 - 22
Опубликована: Дек. 13, 2024
Ovarian
cancer
is
one
of
the
most
aggressive
and
deadly
gynaecological
malignancies
remains
frequently
diagnosed
at
advanced
stages
because
its
asymptomatic
progression
inherent
limitations
current
diagnostic
tests.
Nanoenzymes
(a
class
nanotechnology-based
artificial
enzymes)
have
great
promise
in
addressing
these
challenges.
greatly
improve
sensitivity
specificity
biosensors
including
optical
electrochemical
systems,
with
real-time
high-precision
detection
key
biomarkers
such
as
CA-125,
HE4,
mesothelin.
The
high
accuracy
biosensors,
fluorescence
surface
plasmon
resonance
(SPR)
based
technologies,
for
early-stage
diagnosis,
cost-effective,
portable,
ultra-low
limits
make
them
attractive
alternatives.
Nanoenzyme-based
drug
delivery
systems
like
liposomes,
polymeric
micelles,
Nanocapsules
therapeutic
outcomes
by
allowing
targeted
transport
to
tumor
tissues,
reducing
systemic
toxicity,
overcoming
resistance
treatment.
PEGylated
liposomal
doxorubicin
(Doxil),
a
formulation,
has
been
shown
enhanced
efficacy
platinum-resistant
ovarian
cancer,
reduced
adverse
effects.
Further
theranostic
applications
metallic
nanoparticles
gold
iron
oxide
can
be
realized
using
therapy
imaging.
These
advancements
come
their
challenges,
however,
biological
barriers,
scalability
before
clinical
translation.
Interdisciplinary
research,
validation,
creation
regulatory
frameworks
safety
are
needed
future
progress.
offer
revolutionize
diagnosis
treatment
potential
facilitate
early
detection,
precision,
patient
outcome
while
filling
huge
gaps
approaches.
Язык: Английский