Development and Functional Characterization of Monoclonal Antibodies for Botulinum Neurotoxin Serotype A DOI Creative Commons
Jian Kang,

Qingyu Lv,

Wenhua Huang

и другие.

Foods, Год журнала: 2025, Номер 14(10), С. 1743 - 1743

Опубликована: Май 14, 2025

Botulinum neurotoxin serotype A (BoNT/A), the most toxic of seven serotypes produced by Clostridium botulinum, poses significant public health risks because its involvement in foodborne outbreaks and potential use bioterrorism. In this study, we developed high-affinity monoclonal antibodies for BoNT/A detection using single-cell fluorescence-activated cell sorting nested PCR. The optimized antibody pair demonstrated exceptional sensitivity, detecting recombinant at concentrations as low 0.02 ng/mL with a linear range 0.02-10 ng/mL, while maintaining high specificity against BoNT/B, E, F. Biolayer interferometry confirmed superior binding kinetics, time-resolved fluoroimmunoassay (TRFIA) consistent performance complex food matrices, including ham sausage soybean paste. These rabbit-derived enable ultrasensitive across diverse offering powerful tool safety monitoring biosecurity.

Язык: Английский

Au octahedrons monolayer film SERS substrate coupled with a hybrid metaheuristic algorithm-optimized ELM model: An analytical strategy for rapid and label-free detection of zearalenone in corn oil DOI
Jia-Ji Zhu, Hao Qian,

Afang Zhu

и другие.

Food Chemistry, Год журнала: 2025, Номер 476, С. 143516 - 143516

Опубликована: Фев. 20, 2025

Язык: Английский

Процитировано

0
Fluorescence-activated cell sorting-based efficient screening of monensin monoclonal antibodies and applications in lateral flow immunoassay DOI
Jingjie Huang, Ao Lin,

Yani Gu

и другие.

Talanta, Год журнала: 2025, Номер unknown, С. 128128 - 128128

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Development and Functional Characterization of Monoclonal Antibodies for Botulinum Neurotoxin Serotype A DOI Creative Commons
Jian Kang,

Qingyu Lv,

Wenhua Huang

и другие.

Foods, Год журнала: 2025, Номер 14(10), С. 1743 - 1743

Опубликована: Май 14, 2025

Botulinum neurotoxin serotype A (BoNT/A), the most toxic of seven serotypes produced by Clostridium botulinum, poses significant public health risks because its involvement in foodborne outbreaks and potential use bioterrorism. In this study, we developed high-affinity monoclonal antibodies for BoNT/A detection using single-cell fluorescence-activated cell sorting nested PCR. The optimized antibody pair demonstrated exceptional sensitivity, detecting recombinant at concentrations as low 0.02 ng/mL with a linear range 0.02-10 ng/mL, while maintaining high specificity against BoNT/B, E, F. Biolayer interferometry confirmed superior binding kinetics, time-resolved fluoroimmunoassay (TRFIA) consistent performance complex food matrices, including ham sausage soybean paste. These rabbit-derived enable ultrasensitive across diverse offering powerful tool safety monitoring biosecurity.

Язык: Английский

Процитировано

0