Screening of Solvent Systems for Countercurrent Chromatography Separation of Polar Constituents from Ginkgo biloba L. Seeds

Molecules, Год журнала: 2025, Номер 30(2), С. 409 - 409

Опубликована: Янв. 19, 2025

The separation of large polar constituents presents a substantial challenge in natural product research when employing column chromatography techniques, as the process is both complex and time-consuming. In this study, an acetonitrile/tetrahydrofuran/di-(2-ethylhexyl) phosphoric acid/aqueous saturated sodium chloride solvent system was developed utilized for countercurrent from Ginkgo biloba L. seeds. Five were effectively isolated using (2:2:0.8:3, v/v) two-step method. initial process, three successfully purified methanol extract: compound 1, 4, 5. Compounds 2 3, co-eluted column, further subjected to inner-recycling chromatographic procedures. At last, five identified, including 4′-O-methylpyridoxine (1); two indole alkaloid N-glucosides, ginkgoside B (2) A (3); 2-(4-hydroxybenzyl) malic acid (4); coniferyl alcohol (5). results demonstrated that serves feasible efficient components.

Язык: Английский

Research progress on extraction of flavonoids with deep eutectic solvents from natural products

Journal of Molecular Liquids, Год журнала: 2025, Номер unknown, С. 127356 - 127356

Опубликована: Март 1, 2025

Язык: Английский