Paradox lost: Concerted evolution and centromeric instability DOI
David Haig

BioEssays, Год журнала: 2022, Номер 44(8)

Опубликована: Июнь 24, 2022

Homologous centromeres compete for segregation to the secondary oocyte nucleus at female meiosis I. Centromeric repeats also with each other populate in mitotic cells of germline and have become adapted use recombinational machinery present promote their own propagation. Repeats are not needed centromeres, rather appear be hospitable habitats colonization proliferation repeats. This is probably an indirect consequence two distinctive features centromeric DNA. Centromeres subject breakage by mechanical forces exerted microtubules meiotic crossing-over suppressed. proteins acting trans under selection mitigate costs cis. Collateral competition may help explain high rates aneuploidy observed early human embryos.

Язык: Английский

Genome control by SMC complexes DOI
Claire Hoencamp, Benjamin D. Rowland

Nature Reviews Molecular Cell Biology, Год журнала: 2023, Номер 24(9), С. 633 - 650

Опубликована: Май 25, 2023

Язык: Английский

Процитировано

61
Vertebrate centromeres in mitosis are functionally bipartite structures stabilized by cohesin DOI Creative Commons
Carlos Sacristán, Kumiko Samejima,

Lorena Andrade Ruiz

и другие.

Cell, Год журнала: 2024, Номер 187(12), С. 3006 - 3023.e26

Опубликована: Май 13, 2024

Centromeres are scaffolds for the assembly of kinetochores that ensure chromosome segregation during cell division. How vertebrate centromeres obtain a three-dimensional structure to accomplish their primary function is unclear. Using super-resolution imaging, capture-C, and polymer modeling, we show partitioned by condensins into two subdomains mitosis. The bipartite found in human, mouse, chicken cells therefore fundamental feature centromeres. Super-resolution imaging electron tomography reveal assemble kinetochores, with each subdomain binding distinct microtubule bundle. Cohesin links centromere subdomains, limiting separation response spindle forces avoiding merotelic kinetochore-spindle attachments. Lagging chromosomes cancer divisions frequently have attachments which separated bioriented. Our work reveals aspect biology implications understanding mechanisms guarantee faithful segregation.

Язык: Английский

Процитировано

16
Specialized replication mechanisms maintain genome stability at human centromeres DOI
Andrea Scelfo, Annapaola Angrisani, Marco Grillo

и другие.

Molecular Cell, Год журнала: 2024, Номер 84(6), С. 1003 - 1020.e10

Опубликована: Фев. 14, 2024

Язык: Английский

Процитировано

8
Satellite DNA shapes dictate pericentromere packaging in female meiosis DOI
Damian Dudka, Jennine M. Dawicki-McKenna,

Xueqi Sun

и другие.

Nature, Год журнала: 2025, Номер unknown

Опубликована: Янв. 8, 2025

Язык: Английский

Процитировано

1
Non–B-form DNA tends to form in centromeric regions and has undergone changes in polyploid oat subgenomes DOI Creative Commons
Qian Liu, Congyang Yi, Z-Y Zhang

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2022, Номер 120(1)

Опубликована: Дек. 27, 2022

Centromeres are the specialized regions of chromosomes that direct faithful chromosome segregation during cell division. Despite their functional conservation, centromeres display features rapidly evolving DNA and wide evolutionary diversity in size organization. Previous work found noncanonical B-form structures abundant several eukaryotic species with a possible implication for centromere specification. Thus far, systematic studies into organization function non-B-form plants remain scarce. Here, we applied oat system to investigate role centromeres. We conducted chromatin immunoprecipitation sequencing using an antibody centromere-specific histone H3 variant (CENH3); this accurately positioned different ploidy levels identified series sequences including minisatellites retrotransposons. To define genetic characteristics centromeres, surveyed repeat dyad symmetries were predicted form non-B-DNA vivo. These bent DNA, slipped Z-DNA, G-quadruplexes, R-loops prone within CENH3-binding regions. Dynamic conformational changes occurred evolution from diploid tetraploid hexaploid oat. Furthermore, single-molecule technique AFM DNA:RNA deep validate R-loop enrichment Centromeric retrotransposons exhibited strong associations formation. Taken together, our study elucidates fundamental character genome reveals its potential

Язык: Английский

Процитировано

29
Conserved and divergent mechanisms of inner kinetochore assembly onto centromeric chromatin DOI
Stanislau Yatskevich, David Barford, Kyle Muir

и другие.

Current Opinion in Structural Biology, Год журнала: 2023, Номер 81, С. 102638 - 102638

Опубликована: Июнь 20, 2023

Язык: Английский

Процитировано

17
DNA satellite and chromatin organization at mouse centromeres and pericentromeres DOI Creative Commons
Jenika Packiaraj, Jitendra Thakur

Genome biology, Год журнала: 2024, Номер 25(1)

Опубликована: Фев. 20, 2024

Abstract Background Centromeres are essential for faithful chromosome segregation during mitosis and meiosis. However, the organization of satellite DNA chromatin at mouse centromeres pericentromeres is poorly understood due to challenges assembling repetitive genomic regions. Results Using recently available PacBio long-read sequencing data from C57BL/6 strain, we find that contrary previous reports their homogeneous nature, both centromeric minor satellites pericentromeric major exhibit a high degree variation in sequence within between arrays. While most arrays continuous, significant fraction interspersed with non-satellite sequences, including transposable elements. immunoprecipitation (ChIP-seq), occupancy CENP-A H3K9me3 pericentric regions, respectively, associated increased enrichment homogeneity these The elements regions not part functional as they lack enrichment. Furthermore, nucleosomes occupy spanning centromeric-pericentric junctions low yet amount spreads locally centromere on telocentric sides. Finally, while display well-phased arrays, phased. Interestingly, class also phase H3K27me3 nucleosomes, indicating nucleosome phasing an inherent property satellites. Conclusions Our findings reveal diversity sequence, organization, structure.

Язык: Английский

Процитировано

6
Enrichment of Non-B-Form DNA at D. melanogaster Centromeres DOI Creative Commons
Venkata S. P. Patchigolla, Barbara G. Mellone

Genome Biology and Evolution, Год журнала: 2022, Номер 14(5)

Опубликована: Апрель 19, 2022

Centromeres are essential chromosomal regions that mediate the accurate inheritance of genetic information during eukaryotic cell division. Despite their conserved function, centromeres do not contain DNA sequences and instead epigenetically marked by presence centromere-specific histone H3 variant centromeric protein A. The functional contribution to centromere identity remains elusive. Previous work found dyad symmetries with a propensity adopt noncanonical secondary structures enriched at several species. These findings lead proposal may contribute specification. Here, we analyze predicted recently identified Drosophila melanogaster. Although only on Y centromere, find other types structures, including melted G-quadruplexes, common features all D. melanogaster centromeres. Our is consistent previous models suggesting be possible implications for

Язык: Английский

Процитировано

26
Interactions of small molecules with DNA junctions DOI Creative Commons
Kane T. McQuaid, Angélique Pipier, Christine J. Cardin

и другие.

Nucleic Acids Research, Год журнала: 2022, Номер 50(22), С. 12636 - 12656

Опубликована: Ноя. 16, 2022

The four natural DNA bases (A, T, G and C) associate in base pairs (A=T G≡C), allowing the attached strands to assemble into canonical double helix of (or duplex-DNA, also known as B-DNA). intrinsic supramolecular properties nucleobases make other associations possible (such triplets or quartets), which thus translates a diversity structures beyond B-DNA. To date, alphabet is ripe with approximately 20 letters (from A- Z-DNA); however, only few them are being considered key players cell biology and, by extension, valuable targets for chemical intervention. In present review, we summarise what about alternative (what they? When, where how do they fold?) proceed discuss further those nowadays therapeutic targets. We more detail molecular tools (ligands) that have been recently developed target these structures, particularly three- four-way junctions, order intervene biological processes involved. This new stimulating playground allows devising innovative strategies fight against genetic diseases.

Язык: Английский

Процитировано

25
Independence of Centromeric and Pericentromeric Chromatin Stability on CCAN Components DOI
Ronald Biggs, Mingxuan Sun, Kousik Sundararajan

и другие.

Molecular Biology of the Cell, Год журнала: 2025, Номер 36(4)

Опубликована: Фев. 12, 2025

The chromatin of the centromere provides assembly site for mitotic kinetochore that couples microtubule attachment and force production to chromosome movement in mitosis. is specified by nucleosomes containing histone H3 variant, CENP-A. constitutive centromeric-associated network (CCAN) are assembled on CENP-A enable separation. surrounded pericentromeric heterochromatin, which itself bound sequence specific binding protein, CENP-B. We performed mechanical experiments chromosomes while tracking CENP-B observe centromere's stiffness role CCAN. degraded CENP-C CENP-N auxin-inducible degrons, we verified compromises CCAN via observation CENP-T loss. Chromosome stretching revealed domain does not visibly stretch, even absence and/or CENP-N. Pericentromeric deforms upon application, ∼3-fold less than entire chromosome. loss has no impact pericentromere stretching. Chromosome-disconnecting nuclease treatments showed structural effects Our show core-centromeric more resilient likely mechanically disconnected from underlying chromatin, pericentric deformable yet stiffer arms.

Язык: Английский

Процитировано

0