Genome-wide mapping of mesoscale neuronal RNA organization and condensation

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Апрель 20, 2025

SUMMARY Subcellular RNA organization can affect critical cellular functions. However, our understanding of microenvironments, particularly biomolecular condensates, remains limited, largely due to a lack technologies comprehensively interrogate mesoscale organization. Here, we adapt Split-Pool Recognition Interactions by Tag Extension map micron-scale RNA-RNA spatial proximity genome-wide across cell regions (RNA-SPRITE). Deploying RNA-SPRITE, find extensive, conserved mature mRNAs, with increased colocalization between mRNAs that share RNA-binding protein (RBP) motifs or encode functionally related proteins. Both effects are especially strong in dendrites and axons, suggesting prevalent mRNA co-regulation. Moreover, less compact folding, lower translation efficiency, specific RBP more likely be RNA-rich condensates. perturbations broadly dissolve enhance condensation reveal motif encoded protein-mediated colocalizations remain intact, independent condensation. These results demonstrate the power RNA-SPRITE revealing aspects RNA’s functional In Brief Unbiased, maps uncover extensive subcellular its governing principles. Highlights reveals specificity is driven shared function distinct protein-binding condensates Neurites have high degree sequence function-dependent

Язык: Английский

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The role of mitochondrial mRNA translation in cellular communication

Journal of Cell Science, Год журнала: 2025, Номер 138(9)

Опубликована: Май 1, 2025

ABSTRACT Mitochondria are dynamic and heterogeneous organelles that rewire their network metabolic functions in response to changing cellular needs. To this end, mitochondria integrate a plethora of incoming signals influence cell fate survival. A crucial highly regulated node cell–mitochondria communication is the translation nuclear-encoded mitochondrial mRNAs. By controlling monitoring spatio-temporal these mRNAs, cells can rapidly adjust function meet demands, optimise ATP production regulate organelle biogenesis turnover. In Review, we focus on how RNA-binding proteins recognise mRNAs acutely modulate rate nutrient availability. We further discuss relevance localised for subsets polarised cells. Finally, highlight quality control mechanisms monitor process at surface connections mitophagy stress responses. propose processes collectively contribute specialisation signalling function.

Язык: Английский

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Regulation of synaptic mitochondria by extracellular vesicles and its implications for neuronal metabolism and synaptic plasticity

Journal of Cerebral Blood Flow & Metabolism, Год журнала: 2025, Номер unknown

Опубликована: Май 14, 2025

Mitochondrial metabolism in neurons is necessary for energetically costly processes like synaptic transmission and plasticity. As post-mitotic cells, are therefore faced with the challenge of maintaining healthy functioning mitochondria throughout lifetime. The precise mechanisms mitochondrial maintenance neurons, particularly morphologically complex dendrites axons, not fully understood. Evidence from several biological systems suggests regulation cellular by extracellular vesicles (EVs), secretory lipid-enclosed that have emerged as important mediators cell communication. In nervous system, neuronal glial EVs were shown to regulate circuit development function, at least part via transfer protein RNA cargo. Interestingly, been implicated diseases characterized altered metabolism, such cancer neurodegenerative diseases. Furthermore, system contain proteins related metabolic processes, even intact mitochondria. Here, we present current knowledge underlying maintenance, highlight recent evidence suggesting EVs. We further discuss potential implications EV-mediated function

Язык: Английский

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Centriolar satellites are sites of translation of centrosomal and ciliary proteins

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 22, 2024

Abstract Centriolar satellites are cytoplasmic particles found in the vicinity of centrosomes and cilia whose functional contribution to formation these cellular structures has long been unclear. By characterizing main scaffolding component satellites, PCM1 or Combover Drosophila , we show that not involved trafficking as previously thought but rather act sites for coordinate translation centrosomal ciliary proteins through interaction with a set RNA binding quality control. Strikingly, concentration near vertebrates is conserved feature therefore dispensable satellite function. Such synthesis may be general eukaryotic cells facilitate protein complex compartmentalization. One-Sentence Summary proteins.

Язык: Английский

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Molecular cartography within axons

Current Opinion in Cell Biology, Год журнала: 2024, Номер 88, С. 102358 - 102358

Опубликована: Апрель 12, 2024

Язык: Английский

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