Frontiers in Cell and Developmental Biology,
Год журнала:
2023,
Номер
11
Опубликована: Май 17, 2023
Enhancers
are
a
class
of
cis-regulatory
elements
in
the
genome
that
instruct
spatiotemporal
transcriptional
program.
Last
decade
has
witnessed
an
exploration
non-coding
transcripts
pervasively
transcribed
from
active
enhancers
diverse
contexts,
referred
to
as
enhancer
RNAs
(eRNAs).
Emerging
evidence
unequivocally
suggests
eRNAs
important
layer
regulation.
In
this
mini-review,
we
summarize
well-established
regulatory
models
for
eRNA
actions
and
highlight
recent
insights
into
structure
chemical
modifications
underlying
their
functions.
We
also
explore
potential
roles
condensates.
Science,
Год журнала:
2023,
Номер
381(6664), С. 1331 - 1337
Опубликована: Сен. 21, 2023
Polycomb
repressive
complex
2
(PRC2)
silences
genes
through
trimethylation
of
histone
H3K27.
PRC2
associates
with
numerous
precursor
messenger
RNAs
(pre-mRNAs)
and
long
noncoding
(lncRNAs)
a
binding
preference
for
G-quadruplex
RNA.
In
this
work,
we
present
3.3-Å-resolution
cryo–electron
microscopy
structure
bound
to
Notably,
RNA
mediates
the
dimerization
by
both
protomers
inducing
protein
interface
composed
two
copies
catalytic
subunit
EZH2,
thereby
blocking
nucleosome
DNA
interaction
H3
tail
accessibility.
Furthermore,
an
RNA-binding
loop
EZH2
facilitates
handoff
between
DNA,
another
activity
implicated
in
regulation
We
identified
gain-of-function
mutation
that
activates
zebrafish.
Our
results
reveal
mechanisms
RNA-mediated
chromatin-modifying
enzyme.
Molecular Cell,
Год журнала:
2024,
Номер
84(7), С. 1271 - 1289.e12
Опубликована: Фев. 21, 2024
Polycomb
repressive
complex
2
(PRC2)
is
reported
to
bind
many
RNAs
and
has
become
a
central
player
in
reports
of
how
long
non-coding
(lncRNAs)
regulate
gene
expression.
Yet,
there
growing
discrepancy
between
the
biochemical
evidence
supporting
specific
lncRNA-PRC2
interactions
functional
demonstrating
that
PRC2
often
dispensable
for
lncRNA
function.
Here,
we
revisit
RNA
binding
by
show
may
not
occur
vivo.
Using
denaturing
purification
vivo
crosslinked
RNA-protein
complexes
human
mouse
cell
lines,
observe
loss
detectable
chromatin-associated
proteins
previously
(CTCF,
YY1,
others),
despite
accurately
mapping
bona
fide
RNA-binding
sites
across
others
(SPEN,
TET2,
others).
Taken
together,
these
results
argue
critical
re-evaluation
broad
role
orchestrate
various
chromatin
regulatory
mechanisms.
Journal of Biological Chemistry,
Год журнала:
2024,
Номер
300(5), С. 107256 - 107256
Опубликована: Апрель 2, 2024
Transforming
growth
factor
β
(TGF-β)
is
a
pleiotropic
cytokine
that
widely
distributed
throughout
the
body.
Its
receptor
proteins,
TGF-β
type
I
and
II
receptors,
are
also
ubiquitously
expressed.
Therefore,
regulation
of
various
signaling
outputs
in
context-dependent
manner
critical
issue
this
field.
Smad
proteins
were
originally
identified
as
signal-activated
transcription
factors
similar
to
signal
transducer
activator
proteins.
Smads
activated
by
serine
phosphorylation
mediated
intrinsic
dual
specificity
kinases
family,
indicating
receptor-restricted
effector
molecules
downstream
ligands
family.
have
other
functions
addition
transcriptional
regulation,
including
post-transcriptional
micro-RNA
processing,
pre-mRNA
splicing,
m6A
methylation.
Recent
technical
advances
novel
landscape
Smad-dependent
transduction,
mitochondrial
function
without
involving
gene
expression.
receptor-activated
act
intracellular
modulators
with
multiple
modes
function.
In
review,
we
discuss
role
beyond.
We
describe
functional
differences
between
Smad2
Smad3,
two
TGF-β,
activin,
myostatin,
differentiation
(GDF)
11,
Nodal.
Trends in Cell Biology,
Год журнала:
2024,
Номер
unknown
Опубликована: Март 1, 2024
Alternative
transcription
start
site
usage
(ATSS)
is
a
widespread
regulatory
strategy
that
enables
genes
to
choose
between
multiple
genomic
loci
for
initiating
transcription.
This
mechanism
tightly
controlled
during
development
and
often
altered
in
disease
states.
In
this
review,
we
examine
the
growing
evidence
highlighting
role
sites
(TSSs)
regulation
of
mRNA
isoform
selection
after
We
discuss
how
choice
initiation
influences
RNA
processing
importance
crosstalk
cell
identity
organism
function.
also
speculate
on
possible
mechanisms
underlying
integration
transcriptional
post-transcriptional
processes.
Molecular Cell,
Год журнала:
2024,
Номер
84(13), С. 2542 - 2552.e5
Опубликована: Май 31, 2024
Integrator
is
a
multi-subunit
protein
complex
responsible
for
premature
transcription
termination
of
coding
and
non-coding
RNAs.
This
achieved
via
two
enzymatic
activities,
RNA
endonuclease
phosphatase,
acting
on
the
promoter-proximally
paused
polymerase
Ⅱ
(RNAPⅡ).
Yet,
it
remains
unclear
how
assembly
recruitment
are
regulated
what
functions
many
its
core
subunits
are.
Here,
we
report
structures
human
sub-complexes:
INTS10/13/14/15
INTS5/8/10/15,
an
integrative
model
fully
assembled
bound
to
RNAPⅡ
elongating
(PEC).
An
in
silico
protein-protein
interaction
screen
over
1,500
factors
(TFs)
identified
ZNF655
as
direct
interacting
partner
INTS13
within
Integrator.
We
propose
wherein
acts
platform
TFs
that
could
modulate
stability
Integrator's
association
at
specific
loci
regulate
attenuation
target
genes.
