Ubiquitination mediates protein localization in RNA virus-infected cells

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 15, 2024

Abstract Viruses trigger monocytes’ proinflammatory and antiviral responses. Ubiquitination, a post-translational modification primarily marking proteins for degradation, regulates cellular responses to virus infection. However, comprehensive analysis of virus-induced ubiquitination in monocytes is lacking. Here we identified widespread increase under viral RNA challenge or influenza infection monocytes. Systematic proteome studies revealed that elicits dynamic ubiquitinome alterations, with notable transition from early late stage. Most this increased not proteolytic targets involved subcellular localization, such as the mitochondrial protein COA7 which, when ubiquitinated during infection, translocates nucleus inhibits stress granules formation TNF-α expression. Blocking halts ribonucleoprotein’s nuclear export, highlighting ubiquitination’s importance localization

Язык: Английский

---

The composition and unrevealed immune role of non-RLR DExD/H box RNA helicases in fish

Comparative Immunology Reports, Год журнала: 2024, Номер unknown, С. 200172 - 200172

Опубликована: Сен. 1, 2024

Язык: Английский

---

Comprehensive in silico characterization of Arabidopsis thaliana RecQl helicases through structure prediction and molecular dynamics simulations

Journal of King Saud University - Science, Год журнала: 2024, Номер 36(10), С. 103479 - 103479

Опубликована: Окт. 10, 2024

Язык: Английский

---

A Novel Peptide Encoded by circSRCAP Confers Resistance to Enzalutamide by Inhibiting the Ubiquitin-Dependent Degradation of AR-V7 in Castration-Resistant Prostate Cancer

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 15, 2024

This study aimed to elucidate mechanisms underlying enzalutamide (ENZ) resistance in castration-resistant prostate cancer (CRPC) by investigating the role of circular RNA circSRCAP. Utilizing high-throughput sequencing, circSRCAP was identified as significantly upregulated ENZ-resistant CRPC cells, correlating with elevated levels androgen receptor splice variant-7 (AR-V7) protein. Further analyses revealed that encodes circSRCAP-75aa, a peptide disrupts AR-V7 regulation inhibiting ubiquitination HSP70, co-chaperone protein, through dissociating STUB1, ubiquitin E3 ligase. mechanism ultimately leads enhanced expression and consequent ENZ. Xenograft tumor models confirmed progression its potential therapeutic target for CRPC. These findings highlight crucial epigenetic regulator determining fate offer promising avenue addressing ENZ

Язык: Английский

---

TRAIP enhances progression of tongue squamous cell carcinoma through EMT and Wnt/β-catenin signaling by interacting with DDX39A

BMC Cancer, Год журнала: 2024, Номер 24(1)

Опубликована: Дек. 2, 2024

Tongue squamous cell carcinoma (TSCC) is one of the most common malignant tumors with high mortality and poor prognosis. Its incidence rate increasing gradually. Tumor necrosis factor receptor-associated interacting protein (TRAIP), as a related to several tumors, reveals that its gene expression different between normal tissue primary tumor head neck using bioinformatics analysis. In our study, TCGA database, immunohistochemistry, proliferation assay, colony formation, wound healing Transwell, cycle analysis xenografts model were used determine functions TRAIP in TSCC. We found may promote proliferation, migration invasion Furthermore, results analysis, mass spectrometry co-immunoprecipitation suggested DDX39A be protein. has been proven an oncogene which have important effect on metastasis multiple tumors. addition, implies prognosis patients. Our study demonstrated probably interact regulate progression through epithelial-mesenchymal transition Wnt/β-catenin pathway. we show necessary domain for interaction region. These indicate occurrence development TSCC expected become new promising therapeutic target.

Язык: Английский

---

Dynamic control of RNA-DNA hybrid formation orchestrates DNA2 activation at stalled forks by RNAPII and DDX39A

Molecular Cell, Год журнала: 2024, Номер unknown

Опубликована: Дек. 1, 2024

Язык: Английский

---

The lipopeptide Pam3CSK4 inhibits Rift Valley fever virus infection and protects from encephalitis

PLoS Pathogens, Год журнала: 2024, Номер 20(6), С. e1012343 - e1012343

Опубликована: Июнь 27, 2024

Rift Valley fever virus (RVFV) is an encephalitic bunyavirus that can infect neurons in the brain. There are no approved therapeutics protect from RVFV encephalitis. Innate immunity, first line of defense against infection, canonically antagonizes viruses through interferon signaling. We found interferons did not efficiently primary cortical RVFV, unlike other cell types. To identify alternative neuronal antiviral pathways, we screened innate immune ligands and discovered TLR2 ligand Pam 3 CSK 4 inhibited bunyaviruses. Mechanistically, blocks viral fusion, independent TLR2. In a mouse model encephalitis, treatment protected animals infection mortality. Overall, fusion inhibitor active brain, representing new approach toward development treatments for infections.

Язык: Английский

---

Control of DDX39a helicase by NOD2.

Опубликована: Сен. 23, 2023

We provide evidence here from overexpression-based gain-of-function studies in human cells (1, 2) which demonstrate control of DExD-box helicase DDX39a by the NLR (nucleotide-binding oligomerization domain-like receptor) family protein NOD2.

Язык: Английский

---

The Evil DExH/D: Chikungunya virus runs but cannot hide from DDX39A

Molecular Cell, Год журнала: 2023, Номер 83(22), С. 3948 - 3949

Опубликована: Ноя. 1, 2023

Язык: Английский

---