International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(2), С. 1313 - 1313
Опубликована: Янв. 21, 2024
WD40
repeat
proteins
(WDRs)
are
present
in
all
eukaryotes
and
include
members
that
implicated
numerous
cellular
activities.
They
act
as
scaffold
thus
molecular
"hubs"
for
protein-protein
interactions,
which
mediate
the
assembly
of
multifunctional
complexes
regulate
key
developmental
processes
Arabidopsis
thaliana,
such
flowering
time,
hormonal
signaling,
stress
responses.
Despite
their
importance,
many
aspects
putative
functions
have
not
been
elucidated
yet.
Here,
we
show
late-flowering
phenotype
anthesis
promoting
factor
1
(aprf1)
mutants
is
temperature-dependent
can
be
suppressed
when
plants
grown
under
mild
heat
conditions.
To
gain
further
insight
into
mechanism
APRF1
function,
employed
a
co-immunoprecipitation
(Co-IP)
approach
to
identify
its
interaction
partners.
We
provide
first
interactome
APRF1,
includes
localized
several
subcellular
compartments
diverse
functions.
The
dual
nucleocytoplasmic
localization
ARRF1,
was
validated
through
with
HEAT
SHOCK
PROTEIN
(HSP90.1)
nucleus
HSP90.2
cytoplasm,
indicates
dynamic
versatile
involvement
multiple
biological
processes.
specific
chaperon
HSP90.1
expands
our
knowledge
regarding
epigenetic
regulation
time
A.
thaliana
suggests
existence
delicate
thermoregulated
during
anthesis.
Molecular Cell,
Год журнала:
2024,
Номер
84(12), С. 2255 - 2271.e9
Опубликована: Июнь 1, 2024
The
mechanisms
and
timescales
controlling
de
novo
establishment
of
chromatin-mediated
transcriptional
silencing
by
Polycomb
repressive
complex
2
(PRC2)
are
unclear.
Here,
we
investigate
PRC2
at
Arabidopsis
FLOWERING
LOCUS
C
(FLC),
known
to
involve
co-transcriptional
RNA
processing,
histone
demethylation
activity,
function,
but
so
far
not
mechanistically
connected.
We
develop
test
a
computational
model
describing
proximal
polyadenylation/termination
mediated
the
RNA-binding
protein
FCA
that
induces
H3K4me1
removal
demethylase
FLD.
feeds
back
reduce
polymerase
II
(RNA
Pol
II)
processivity
thus
enhance
early
termination,
thereby
repressing
productive
transcription.
predicts
this
transcription-coupled
repression
controls
level
antagonism
action.
Thus,
effectiveness
dictates
timescale
for
PRC2/H3K27me3
silencing.
experimentally
validate
these
mechanistic
predictions,
revealing
processing
sets
transcription
locus,
which
then
determines
rate
ON-to-OFF
switch
The
restrictor,
ZC3H4/WDR82,
terminates
antisense
transcription
from
bidirectional
promoters,
but
its
mechanism
is
poorly
understood.
We
report
that
ZC3H4/WDR82
immunoprecipitate
with
PP1
phosphatase
and
nuclear
targeting
subunit,
PNUTS,
which
binds
to
WDR82.
AlphaFold
predicts
a
complex
of
PP1/PNUTS
restrictor
where
both
PNUTS
ZC3H4
contact
A
substrate
trap,
PP1H66K-PNUTS,
comprising
inactive
fused
the
C-terminus
antagonizes
mediated
termination
whereas
PP1WT-PNUTS
has
less
effect
suggesting
activity
required
for
termination.
One
implicated
in
by
pol
II
CTD
Ser5-P.
PP1H66K-PNUTS
induces
Ser5-P
hyperphosphorylation
at
5'
ends
presumably
inhibiting
dephosphorylation.
NET-seq
analysis
suggests
Ser5
dephosphorylation
would
promote
increasing
pausing.
Both
inhibition
require
WDR82
binding
domain
mediates
binding.
In
summary,
associated
via
promotes
efficient
Journal of Molecular Biology,
Год журнала:
2024,
Номер
437(1), С. 168707 - 168707
Опубликована: Июль 14, 2024
The
regulation
of
transcription
by
RNA
polymerase
II
(RNAPII)
underpins
all
cellular
processes
and
is
perturbed
in
thousands
diseases.
In
humans,
RNAPII
transcribes
∼20000
protein-coding
genes
engages
apparently
futile
non-coding
at
other
sites.
Despite
being
so
ubiquitous,
this
usually
attenuated
soon
after
initiation
the
resulting
products
are
immediately
degraded
nuclear
exosome.
We
others
have
recently
described
a
new
complex,
"Restrictor",
which
appears
to
control
such
unproductive
transcription.
Underpinned
binding
protein,
ZC3H4,
Restrictor
curtails
unproductive/pervasive
genome-wide.
Here,
we
discuss
these
recent
discoveries
speculate
on
some
many
unknowns
regarding
function
mechanism.
Proceedings of the National Academy of Sciences,
Год журнала:
2024,
Номер
121(47)
Опубликована: Ноя. 15, 2024
Plants
use
seasonal
cold
to
time
the
transition
reproductive
development.
Short-
and
long-term
exposure
is
registered
via
parallel
transcriptional
shutdown
Polycomb-dependent
epigenetic
silencing
of
