RNA Damage and Its Implications in Genome Stability
DNA repair,
Год журнала:
2025,
Номер
147, С. 103821 - 103821
Опубликована: Март 1, 2025
Язык: Английский
NAT10 and N 4 -acetylcytidine restrain R-loop levels and related inflammatory responses
Science Advances,
Год журнала:
2025,
Номер
11(13)
Опубликована: Март 26, 2025
N
4
-acetylcytidine
(ac
C)
is
deposited
on
diverse
RNAs
by
-acetyltransferase
10
(NAT10),
a
protein
with
high
biological
relevance
for
aging
and
cancer.
We
performed
comprehensive
survey
of
ac
C
using
metabolic
labeling,
sodium
cyanoborohydride
chemical
treatment
coupled
to
next-generation
sequencing
(NGS),
antibody–based
cell
molecular
biology
techniques.
Our
analysis
shows
that
NAT10-dependent
C-acetylation
robust
in
rRNA
specific
tRNAs
but
low/spurious
mRNA.
It
also
revealed
an
inflammatory
signature
mutagenesis
at
transcriptionally
active
sites
NAT10-KO
cells.
This
finding
led
us
explore
the
role
NAT10
R-loops,
which
were
recently
linked
APOBEC3B-mediated
mutagenesis.
showed
R-loops
are
C-acetylated
manner.
Furthermore,
restrains
levels
subset
differentially
expressed
genes
catalytic
activity–dependent
Together
cellular
data
showing
C-modified
RNA
endosomal
structures,
we
propose
increased
C-unmodified
RNAs,
likely
derived
from
structures
induce
responses.
Язык: Английский
Sacred codes: preservation, permutation and expression
Biomedical Journal,
Год журнала:
2025,
Номер
unknown, С. 100852 - 100852
Опубликована: Апрель 1, 2025
This
issue
of
the
Biomedical
Journal
features
a
special
section
on
epigenetics
in
infection,
exploring
epitranscriptomic
modifications
malaria
parasite
Plasmodium
and
African
sleeping
sickness
Trypanosoma,
with
focus
how
these
influence
development
survival
strategies.
Additionally,
this
reviews
regulatory
mechanisms
asymmetric
cell
division,
assessment
needs
for
children
developmental
coordination
disorder
along
recommendations,
advancements
3D
chondrocyte
culturing
tissue
engineering.
Also
examined
are
three
key
pathways
exacerbating
long
COVID,
increasing
impact
electromagnetic
exposure
built
environments
human
health,
recent
updates
liver
transplantation
criteria
hepatocellular
carcinoma
complicated
by
portal
vein
tumor
thrombosis.
Research
bladder
cancer
investigates
role
activating
transcription
factor
3,
known
its
anti-tumor
properties,
link
to
metformin.
Another
study
demonstrates
efficacy
Sanger
sequencing
streamlining
mitochondrial
diagnostics,
enabling
timely
identification
based
clinical
evidence.
Additional
studies
present
novel
intervention
approach
autoimmune
diseases,
artificial
bone
grafts
enhance
regeneration,
benefits
public
hospital
participation
oncological
trials.
Finally,
confirms
suitability
transthoracic
echocardiography
diagnosing
suspected
acute
aortic
syndrome.
Язык: Английский
When RNA damage induces DNA breaks
Trends in Cell Biology,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 1, 2025
Язык: Английский
Nuclear accumulation of YTHDF1 regulates mRNA splicing in the DNA damage response
Science Advances,
Год журнала:
2025,
Номер
11(16)
Опубликована: Апрель 16, 2025
YTH
domain–containing
family
protein
1
(YTHDF1),
a
reader
of
N6-methyladenosine
(m
6
A),
has
been
implicated
in
regulating
RNA
metabolism
the
cytosol.
Here,
we
report
role
YTHDF1
within
nucleus
response
to
genotoxic
stress.
Upon
radiation,
is
phosphorylated
at
serine-182
an
ataxia
telangiectasia
and
Rad3-related–dependent
manner.
This
phosphorylation
inhibits
exportin
1–mediated
nuclear
export
YTHDF1,
resulting
its
accumulation
nucleus.
Nuclear
enhances
binding
capacity
serine-
arginine-rich
splicing
factor
2
group
m
A-modified
exons,
leading
increased
exon
inclusion.
Specifically,
promotes
expression
DNA
repair
genes,
such
as
BRCA1
TP53BP1
,
thereby
mitigating
excessive
damage.
Depletion
sensitizes
cancer
cells
radiation
treatment.
Together,
our
study
reveals
crucial
A-mediated
messenger
damage
response,
proposing
it
potential
target
for
therapy.
Язык: Английский