Circular RNAs in neurological conditions – computational identification, functional validation, and potential clinical applications
Molecular Psychiatry,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 17, 2025
Abstract
Non-coding
RNAs
(ncRNAs)
have
gained
significant
attention
in
recent
years
due
to
advancements
biotechnology,
particularly
high-throughput
total
RNA
sequencing.
These
developments
led
new
understandings
of
non-coding
biology,
revealing
that
approximately
80%
regions
the
genome
possesses
biochemical
functionality.
Among
ncRNAs,
circular
(circRNAs),
first
identified
1976,
emerged
as
a
prominent
research
field.
CircRNAs
are
abundant
most
human
cell
types,
evolutionary
conserved,
highly
stable,
and
formed
by
back-splicing
events
which
generate
covalently
closed
ends.
Notably,
circRNAs
exhibit
high
expression
levels
neural
tissue
perform
diverse
functions,
including
acting
molecular
sponges
for
microRNAs,
interacting
with
RNA-binding
proteins
regulate
their
availability
activity,
modulating
transcription
splicing,
even
translating
into
functional
peptides
some
cases.
Recent
computational
experimental
methods
enhanced
our
ability
identify
validate
circRNAs,
providing
valuable
insights
biological
roles.
This
review
focuses
on
circRNA
they
related
neuropsychiatric
neurodegenerative
conditions.
We
also
explore
potential
applications
clinical
diagnostics,
therapeutics,
future
directions.
remain
relatively
underexplored
area
context
neurological
disorders.
However,
emerging
evidence
supports
role
critical
players
etiology
mechanisms
conditions
such
schizophrenia,
bipolar
disorder,
major
depressive
Alzheimer’s
disease,
Parkinson’s
disease.
findings
suggest
may
provide
novel
framework
contributing
dysfunctions
underpinning
these
complex
Язык: Английский
Perspectives of Exosomal ncRNAs in the Treatment of Bone Metabolic Diseases: Focusing on Osteoporosis, Osteoarthritis, and Rheumatoid Arthritis
Experimental Cell Research,
Год журнала:
2025,
Номер
unknown, С. 114457 - 114457
Опубликована: Фев. 1, 2025
Язык: Английский
Multiple roles of circular RNAs in prostate cancer: from the biological basis to potential clinical applications
European journal of medical research,
Год журнала:
2025,
Номер
30(1)
Опубликована: Фев. 27, 2025
Prostate
cancer
is
an
important
health
concern
affecting
men.
Circular
RNAs
(circRNAs)
play
molecular
biological
role
in
regulating
gene
expression
due
to
their
unique
structure.
Studies
have
revealed
the
involvement
of
circRNAs
many
human
diseases.
In
prostate
cancer,
can
act
as
oncogenes
or
tumour
suppressor
genes
and
affect
cell
proliferation,
invasion,
resistance
chemotherapy
and,
consequently,
disease
progression.
Accordingly,
cancer-related
are
expected
serve
new
targets
early
clinical
diagnosis
targeted
therapy,
but
various
roles
not
been
fully
elucidated.
This
article
reviews
pathological
circRNA
explores
its
prospects
a
translational
medicine
treatment
prognostic
evaluation.
Язык: Английский
A Dual-Selection System for Enhanced Efficiency and Fidelity of Circular RNA Overexpression
Journal of Molecular Biology,
Год журнала:
2025,
Номер
unknown, С. 169064 - 169064
Опубликована: Март 1, 2025
Circular
RNAs
(circRNAs)
are
essential
regulators
of
cellular
processes,
but
challenging
to
study
using
traditional
methods.
Overexpression
approaches,
such
as
the
use
linearized
plasmids
and
viral
vectors,
often
result
in
high
rates
false-positive
clones,
where
cells
retain
selection
markers
without
expressing
target
circRNA.
This
addresses
this
limitation
by
developing
a
dual-selection
circRNA
system
designed
enhance
accuracy
reliability
overexpression.
Our
integrates
fluorescent
reporter
gene
upstream
expression
cassette,
under
shared
promoter,
downstream
antibiotic
resistance
marker,
allowing
for
both
flow
cytometric
cell-sorting
identify
enrich
with
genuine
expression.
We
successfully
incorporated
into
an
inducible
lentiviral
vector
controlled
overexpression
various
cell
types.
The
offers
significant
advance
research
studies
other
RNA
species
accurate
reliable
is
essential.
Язык: Английский