Plants
have
been
used
for
the
treatment
of
various
cancers
since
ancient
times.
Breast
cancer
is
second
commonest
global
deadly
menace.
Natural
plant
extracts,
herbal
medicines,
are
generally
accepted
as
safer
drug
substances
than
their
synthetic
counterparts.
Artemisia
absinthium,
a
perennial
bushy
plant,
has
traditionally
beneficial
in
diverse
maladies,
including
hepatocyte
overgrowth,
hepatitis,
gastritis,
jaundice,
wound
healing,
splenomegaly,
dyspepsia,
indigestion,
bloating,
stomach
pain,
anaemia
and
anorexia.
It
also
known
its
antioxidant,
antifungal,
antimicrobial,
anthelmintic,
anti-ulcer,
anticarcinogenic,
hepatoprotective,
neuroprotective,
antidepressant,
analgesic,
immunomodulatory,
cytotoxic
properties.
In
this
study,
methanol
extracts
powdered
leaves
absinthium
was
tried
on
breast
cells.
The
molecular
content
extract
determined
by
GC-MS.
Chemical
components
were
determinated
extract,
inhibitory
activities
these
chemicals
against
Crystal
structure
protein
(PDB
ID:
1A52
1JNX)
downloaded
from
Protein
Data
Bank
site
compared.
Zeitschrift für Naturforschung C,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 13, 2025
Abstract
A
new
series
of
hydrazone
derivatives
(
1a-1l
)
were
prepared
from
a
condensation
reaction
between
different
hydrazide
and
3-formylbenzoic
acid.
Through
the
use
several
spectral
techniques,
such
as
1
H-NMR,
13
C-NMR,
elemental
analysis,
structures
compounds
clarified.
The
crystal
structure
compound
1d
was
obtained
by
single-crystal
X-ray
crystallography.
They
found
to
have
inhibitory
effects
on
anticancer
potentials
human
carbonic
anhydrase
isoforms
I
II.
Compound
be
strongest
inhibitor,
with
IC
50
values
0.133
µM
against
hCA
I.
Also,
1l
showed
highest
activity
3.244
Moreover,
their
cytotoxic
rat
glioma
cell
colon
adeno
carcinoma
lines
evaluated.
According
cytotoxicity
results,
1j
exhibited
HT29
cell,
while
1e
,
1g,
effect
C6
line.
which
carries
methoxy
substituent
at
3
rd
position
phenyl
ring,
effective
both
cancer
cells
ADME/T
properties
molecular
docking
molecules
examined.
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Фев. 18, 2025
Seven
compounds
were
isolated
from
ethyl
acetate
extract
of
Alcea
rosea
and
examined
for
their
cytotoxicity
against
HCT116,
HT29
SW480
colon
cancer
cells.
It
was
found
that
two
(C4
C5)
exhibited
strong
anti-colon
activities.
These
used
to
study
properties
include
MTT
activity
(with
IC50
C4
as
74.71,
129.0
131.4
µg/ml
in
respectively,
whereas
C5
128.1,
168.4
225.8
cells
respectively),
colony
formation
activity,
wound
healing
spheroid
DAPI-PI
staining,
acridine-orange
ethidium
bromide
ROS
measurement,
rhodamine-123
staining
both
HCT116
Both
the
showed
significant
increase
apoptosis
visualized
by
4′,6-diamidino-2-phenylindol/propidium
iodide
(DAPI-PI)
acridine
orange/ethidium
(AO/EtBr)
staining.
The
induction
further
confirmed
expressions
cleaved
PARP
caspase
3.
generation
its
effect
on
MMP
measured
with
Dichloro-dihydro-fluorescein
diacetate
(DCFH-DA)
Rhodamine.
Expression
levels
EMT
associated
markers
like
Cyclin
D1,
Slug,
Vimentin,
E-Cadherin
also
studied.
down
regulate
protein
Vimentin
a
concentration-dependent
manner.
Eeffect
key
signaling
Wnt3a,
Notch1,
Shh
evaluated.
Additionally,
mRNA
these
genes
analyzed.
best
binding
affinity
when
docked
Wnt3a
Notch1.
Similarly,
−
8.8,
-8.2
7.6
kcal⋅mol−
1
Shh,
present
findings
provide
insight
immense
scientific
support
integrity
piece
indigenous
knowledge.
However,
validation
living
organisms
is
necessary
before
progressing
clinical
trials
advancing
it
into
marketable
pharmaceutical
product.
