Freeze-dried fecal microorganisms as an effective biomaterial for the treatment of calves suffering from diarrhea DOI Creative Commons
Jahidul Islam,

Natsuki Ohtani,

Yu Shimizu

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Ноя. 14, 2024

Fecal microbiota transplantation (FMT) is a therapeutic modality for treating neonatal calf diarrhea. Several practical barriers, including donor selection, fecal collection, and limited timeframe FMT, are the main constraints to using fresh feces implementing on-farm FMT. We report utility of FMT with pretreated ready-to-use frozen (F) or freeze-dried (FD) microorganisms In total, 19 (F-FMT, n = 10 FD-FMT, 9) treatments were conducted. Both 100% clinically effective; however, multi-omics analysis showed that FD-FMT was superior F-FMT. Machine learning SourceTracker confirmed retained four times better in recipient calves treated than A predictive model based on receiver operating characteristic curve area under more discriminative F-FMT observed changes metabolites during disease recovery. These results provide new insights into establishing methods preparing increase quality animals may contribute humans.

Язык: Английский

Safety and efficacy of fecal microbiota transplantation (FMT) as a modern adjuvant therapy in various diseases and disorders: a comprehensive literature review DOI Creative Commons
Mohammad M. Karimi, Niyousha Shirsalimi, Zahra Hashempour

и другие.

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Сен. 26, 2024

The human gastrointestinal (GI) tract microbiome is a complex and all-encompassing ecological system of trillions microorganisms. It plays vital role in digestion, disease prevention, overall health. When this delicate balance disrupted, it can lead to various health issues. Fecal microbiota transplantation (FMT) an emerging therapeutic intervention used as adjuvant therapy for many diseases, particularly those with dysbiosis their underlying cause. Its goal restore by transferring fecal material from healthy donors the recipients. FMT has impressive reported cure rate between 80% 90% become favored treatment diseases. While may have generally mild moderate transient adverse effects, rare severe complications underscore importance rigorous donor screening standardized administration. enormous potential practical approach; however, additional research required further determine its clinical utilization, well safety efficiency different patient populations. This comprehensive literature review offers increased confidence effectiveness several diseases affecting intestines other systems, including diabetes, obesity, inflammatory autoimmune illness, conditions.

Язык: Английский

Процитировано

17

From chaos to order: optimizing fecal microbiota transplantation for enhanced immune checkpoint inhibitors efficacy DOI Creative Commons
Anqi Lin, Aimin Jiang, Lihaoyun Huang

и другие.

Gut Microbes, Год журнала: 2025, Номер 17(1)

Опубликована: Янв. 18, 2025

The integration of fecal microbiota transplantation (FMT) with immune checkpoint inhibitors (ICIs) presents a promising approach for enhancing cancer treatment efficacy and overcoming therapeutic resistance. This review critically examines the controversial effects FMT on ICIs outcomes elucidates underlying mechanisms. We investigate how modulates gut composition, microbial metabolite profiles, tumor microenvironment, thereby influencing effectiveness. Key factors efficacy, including donor selection criteria, recipient characteristics, administration protocols, are comprehensively discussed. delineates strategies optimizing formulations systematically monitoring post-transplant microbiome dynamics. Through comprehensive synthesis evidence from clinical trials preclinical studies, we elucidate potential benefits challenges combining across diverse types. While some studies report improved outcomes, others indicate no benefit or adverse effects, emphasizing complexity host-microbiome interactions in immunotherapy. outline critical research directions, encompassing need large-scale, multi-center randomized controlled trials, in-depth ecology multi-omics approaches artificial intelligence. Regulatory ethical addressed, underscoring imperative standardized protocols rigorous long-term safety assessments. seeks to guide future endeavors applications FMT-ICIs combination therapy, improve patient while ensuring both efficacy. As this rapidly evolving field advances, maintaining judicious balance between openness innovation cautious scrutiny is crucial realizing full modulation

Язык: Английский

Процитировано

3

Assessing microbiota in vivo: debugging with medical imaging DOI
Donna E. Goldhawk, Kait F. Al,

Sarah C. Donnelly

и другие.

Trends in Microbiology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0

Digging deeper into necrotizing enterocolitis: bridging clinical, microbial, and molecular perspectives DOI Creative Commons
Deshuang Zhang,

Dongke Xie,

Yi Qu

и другие.

Gut Microbes, Год журнала: 2025, Номер 17(1)

Опубликована: Янв. 18, 2025

Necrotizing Enterocolitis (NEC) is a severe, life-threatening inflammatory condition of the gastrointestinal tract, especially affecting preterm infants. This review consolidates evidence from various biomedical disciplines to elucidate complex pathogenesis NEC, integrating insights clinical, microbial, and molecular perspectives. It emphasizes modulation NEC-associated pathways by probiotics novel biologics, highlighting their therapeutic potential. We further critically examine dysbiotic alterations within gut microbiota, with particular focus on imbalances in bacterial viral communities, which may contribute onset NEC. The intricate interactions among toll-like receptor 4 (TLR4), microvascular integrity, immune activation, milieu are meticulously summarized, offering sophisticated understanding NEC pathophysiology. academic aims enhance etiological comprehension promote development targeted interventions, impart significant impact perinatal factors formulation preventive curative strategies for disease.

Язык: Английский

Процитировано

0

Microbiota transplantation and administration of live biotherapeutic products for the treatment of dysbiosis-associated diseases DOI
Samantha Flores-Treviño, Paola Bocanegra‐Ibarias, Daniel Salas‐Treviño

и другие.

Expert Opinion on Biological Therapy, Год журнала: 2025, Номер unknown

Опубликована: Март 26, 2025

The microbiota composition in humans varies according to the anatomical site and is crucial for maintaining homeostasis an overall healthy state. Several gastrointestinal, vaginal, respiratory, skin diseases are associated with dysbiosis. Alternative therapies such as transplantation can help restore normal be implemented treat clinically relevant diseases. Current conducted clinical trials were included this review (after searching on MEDLINE database from years 2017 2025) fecal (FMT) against recurrent Clostridiodes difficile infection (rCDI) vaginal (VMT) bacterial vaginosis. Washed (WMT) live biotherapeutic products (LBPs) also reviewed. In microbiota-based therapy, selecting optimal donors a limitation. A stool or bank should overcome time-consuming expensive process of donor recruitment. Microbiota-based LBPs promising treatment alternatives rCDI other dysbiosis-associated Specific could engineered out fluids-derived strains achieve selection specific beneficial microorganisms Personalized treatments solutions diseases, which remains important necessity practice.

Язык: Английский

Процитировано

0

Translational strategies for oral delivery of faecal microbiota transplantation DOI
Srinivas Kamath, Robert V. Bryant, Samuel P. Costello

и другие.

Gut, Год журнала: 2025, Номер unknown, С. gutjnl - 335077

Опубликована: Апрель 29, 2025

Faecal microbiota transplantation (FMT) has emerged as a transformative therapy for Clostridioides difficile infections and shows promise various GI systemic diseases. However, the poor patient acceptability accessibility of ‘conventional’ FMT, typically administered via colonoscopies or enemas, hinders its widespread clinical adoption, particularly chronic conditions. Oral administration FMT (OralFMT) overcomes these limitations, yet faces distinct challenges, including significant capsule burden, palatability concerns microbial viability during gastric transit. This review provides comprehensive analysis emerging strategies that aim to advance OralFMT by: (1) refining processing technologies (eg, lyophilisation) enable manufacturing low-volume formulations reducing burden (2) developing delivery improve organoleptic safeguard targeted colonic release. These advancements present opportunities expand therapeutic scope, beyond C. infections, towards conditions requiring frequent dosing regimens. While this primarily focuses on optimising delivery, it is important contextualise within broader shift defined consortia. Live biotherapeutic products (LBPs) offer an alternative approach, interplay between LBPs in practice remains unresolved. We postulate continued innovation multidisciplinary approach can further increase efficacy scalability by enabling disease site targeting, co-delivery compounds overcoming colonisation resistance. Realising goals positions cornerstone personalised care across range diseases rooted microbiome health.

Язык: Английский

Процитировано

0

Altered Expression of Intestinal Tight Junctions in Patients with Chronic Kidney Disease: A Pathogenetic Mechanism of Intestinal Hyperpermeability DOI Creative Commons
Georgia-Andriana Georgopoulou, Marios Papasotiriou,

Pinelopi Bosgana

и другие.

Biomedicines, Год журнала: 2024, Номер 12(2), С. 368 - 368

Опубликована: Фев. 5, 2024

Background: Systemic inflammation in chronic kidney disease (CKD) is associated (as a cause or effect) with intestinal barrier dysfunction and increased gut permeability, mechanisms not yet fully understood. This study investigated different parameters of the CKD patients, especially tight junction (TJ) proteins their possible association systemic endotoxemia inflammation. Methods: Thirty-three patients stage I–IV (n = 17) end-stage (ESKD) 16) 11 healthy controls underwent duodenal biopsy. Samples were examined histologically, presence CD3+ T-lymphocytes expression occludin claudin-1 epithelium was evaluated by means immunohistochemistry, circulating endotoxin concentrations determined ELISA cytokines IL-1β, IL-6, IL-8, IL-10 TNF-α serum measured using flow cytometry. Results: Patients ESKD had significantly higher endotoxin, IL-8 levels compared to controls. Intestinal decreased, inversely correlated endotoxemia. Regarding occludin, specific pattern observed, gradually increasing loss its from crypt tip villi. Conclusion: The enterocytes reduced CKD, contributing inflammatory responses these patients.

Язык: Английский

Процитировано

3

Fecal microbiota transplantation as a possibility of curing Irritable Bowel Syndrome FMT as a possibility of curing IBS DOI
Agnieszka Urbanek, Urszula Guderska, Jacek Kurzeja

и другие.

Progress in Health Sciences, Год журнала: 2024, Номер 14(1), С. 41 - 49

Опубликована: Июнь 19, 2024

Introduction and Objective. Irritable Bowel Syndrome (IBS) became recently a common problem among the population of highly developed countries. Most patients suffer from chronic abdominal pain various bowel habit disorders. Although pathogenesis IBS was not yet entirely defined, intestinal dysbiosis is frequently mentioned as one most possible mechanisms. There may be way to permanently cure IBS, by replacing patient’s dysbiotic microflora with Fecal Microbiota Transplantation (FMT) healthy donor. The purpose this paper present FMT method treatment.Review methods. Search using PubMed database combined keywords: "Irritable Syndrome", "Fecal Transplantation", "Dysbiosis" "Intestinal Microbiota". An attempt made comprehensive state knowledge while indicating areas that require further research. Brief description knowledge. Effectiveness in has been clinically proven seems donor-dependent. Superdonor’s microbiota profile defined yet. gradually gaining popularity new research appears confirming long-term effectiveness procedure.Summary. Research results are promising it likely will introduced treatment routinely used clinical practice. Differences test related use material donors. It important establish exact dosage, frequency procedure, criteria for selecting superdonors, if more than donor should used, other things.

Язык: Английский

Процитировано

0

Fecal microbiota transplantation for glaucoma; a potential emerging treatment strategy DOI Creative Commons
Rasoul Ebrahimi, Yeganeh Farsi, Seyed Aria Nejadghaderi

и другие.

Current Research in Microbial Sciences, Год журнала: 2024, Номер 7, С. 100314 - 100314

Опубликована: Янв. 1, 2024

Glaucoma is the primary cause of irreversible blindness globally. Different glaucoma subtypes are identified by their underlying mechanisms, and treatment options differ its pathogenesis. Current management includes topical medications to lower intraocular pressure surgical procedures like trabeculoplasty drainage implants. Fecal microbiota transplantation (FMT) an almost effective safe option for recurrent Clostridium difficile infection. The relationship between bacterial populations, metabolites, inflammatory pathways in retinal diseases indicates possible therapeutic strategies. Thus, incorporating host microbiota-based therapies could offer additional patients. Here, we propose that combining FMT with standard treatments may benefit those affected this condition. Also, potential safety, efficacy, cost-effectiveness clinical applications discussed.

Язык: Английский

Процитировано

0

Freeze-dried fecal microorganisms as an effective biomaterial for the treatment of calves suffering from diarrhea DOI Creative Commons
Jahidul Islam,

Natsuki Ohtani,

Yu Shimizu

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Ноя. 14, 2024

Fecal microbiota transplantation (FMT) is a therapeutic modality for treating neonatal calf diarrhea. Several practical barriers, including donor selection, fecal collection, and limited timeframe FMT, are the main constraints to using fresh feces implementing on-farm FMT. We report utility of FMT with pretreated ready-to-use frozen (F) or freeze-dried (FD) microorganisms In total, 19 (F-FMT, n = 10 FD-FMT, 9) treatments were conducted. Both 100% clinically effective; however, multi-omics analysis showed that FD-FMT was superior F-FMT. Machine learning SourceTracker confirmed retained four times better in recipient calves treated than A predictive model based on receiver operating characteristic curve area under more discriminative F-FMT observed changes metabolites during disease recovery. These results provide new insights into establishing methods preparing increase quality animals may contribute humans.

Язык: Английский

Процитировано

0