Epigenetics in autoimmune diseases: Unraveling the hidden regulators of immune dysregulation
Autoimmunity Reviews,
Год журнала:
2025,
Номер
24(6), С. 103784 - 103784
Опубликована: Март 3, 2025
Autoimmune
diseases
result
from
complex
interactions
between
genetic
and
environmental
factors.
Recent
advances
in
epigenetic
research
shed
light
on
the
intricate
regulatory
mechanisms
that
contribute
to
development
progression
of
such
conditions.
The
present
review
aims
explore
role
modifications,
including
DNA
methylation,
histone
non-coding
RNAs,
context
autoimmune
diseases.
We
discuss
current
understanding
alterations
associated
with
various
disorders,
their
impact
immune
cell
function,
potential
as
innovative
therapeutic
targets.
Additionally,
we
highlight
main
future
directions
field
epigenetics
autoimmunity.
Язык: Английский
Start small, think big: MicroRNAs in diabetes mellitus and relevant cardiorenal-liver metabolic health spectrum
Metabolism,
Год журнала:
2025,
Номер
165, С. 156153 - 156153
Опубликована: Фев. 4, 2025
Язык: Английский
The diagnostic value of a breast cancer diagnosis model based on serum MiRNAs and serum tumor markers
World Journal of Surgical Oncology,
Год журнала:
2025,
Номер
23(1)
Опубликована: Март 29, 2025
Abstract
Background
Breast
cancer
(BCa)
is
the
leading
cause
of
cancer-related
death
among
women
worldwide.
MicroRNAs
(miRNAs)
are
promising
tools
for
diagnosis
and
prognosis.
This
study
investigated
role
serum
miRNAs
tumor
markers
(TMs)
in
BCa.
Methods
Differentially
expressed
were
screened
from
samples
BCa
patients
healthy
individuals
via
high-throughput
sequencing.
The
expression
hsa-miR-1911-3p,
hsa-miR-4694-5p,
hsa-miR-548ao-5p,
hsa-miR-4804-3p
169
116
controls
was
detected
qRT-PCR.
Serum
tumor-associated
antigens
by
chemiluminescence.
Logistic
regression
subsequently
used
to
develop
miRNA
panel
I,
TM
II,
(miRNA
+
TM)
III
models.
Receiver
operating
characteristic
(ROC)
curve,
precision-recall
(PR)
curve
decision
analyses
(DCA)
performed
assess
accuracy
three
models
diagnosis.
Additionally,
relationships
between
clinical
characteristics
with
assessed.
Results
Four
(hsa-miR-1911-3p,
hsa-miR-4804-3p)
newly
associated
I
based
on
hsa-miR-548ao-5p
showed
greater
diagnostic
effectiveness
than
II
antigen
125
(CA125)
153
(CA153),
AUC
values
0.816
0.777,
respectively.
had
higher
an
value
0.870.
miR-548ao-5p
miR-4804-3p
closely
related
features,
such
as
human
epidermal
growth
factor
receptor
2
(HER2),
estrogen
(ER),
progesterone
(PR),
HER2-enriched
subtype,
stage
III/IV,
lymph
node-transplanted
breast
cancer.
Conclusion
MiR-548ao-5p
miR-4804-3
could
serve
potential
biomarkers
Язык: Английский
Identification of potential causal genes and drug targets in pulmonary hypertension based on transcriptomic analysis and Mendelian randomization
Postgraduate Medical Journal,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 29, 2025
Currently,
there
is
no
definitive
treatment
for
pulmonary
hypertension
(PH).
This
study
aims
to
utilize
the
GEO
database
and
conduct
Mendelian
randomization
(MR)
analysis
identify
new
genetic
targets
PH
investigate
their
potential
pathogenic
pathways
therapeutic
drugs.
We
identified
key
genes
by
combining
findings
from
MR
bioinformatics
analyses
of
datasets.
performed
enrichment
explore
functional
roles
these
genes.
Then,
we
constructed
protein-protein
interaction
(PPI)
miRNA-mRNA
networks
interacting
proteins
miRNAs.
Drug
prediction
was
conducted
propose
Finally,
validated
results
through
dataset,
RT-PCR,
western
blot
experiments.
The
joint
utilizing
databases
two
genes,
ITGA2B
TSPAN9
that
exhibited
significance
across
both
analytical
methods.
indicated
were
involved
in
critical
biological
functions
pathways,
including
cell
adhesion,
platelet
activation,
PI3K-Akt
signaling
pathway.
PPI
further
highlighted
PH.
revealed
as
targets.
RT-PCR
experiments
above
findings.
By
integrating
analysis,
found
have
a
causal
relationship
with
Our
offer
insights
into
molecular
mechanism
PH,
establishing
basis
future
research
clinical
applications.
Язык: Английский
Circulating Non-Coding RNAs as Indicators of Fibrosis and Heart Failure Severity
Cells,
Год журнала:
2025,
Номер
14(7), С. 553 - 553
Опубликована: Апрель 7, 2025
Heart
failure
(HF)
is
a
leading
cause
of
morbidity
and
mortality
worldwide,
representing
complex
clinical
syndrome
in
which
the
heart’s
ability
to
pump
blood
efficiently
impaired.
HF
can
be
subclassified
into
heart
with
reduced
ejection
fraction
(HFrEF)
preserved
(HFpEF),
each
distinct
pathophysiological
mechanisms
varying
levels
severity.
The
progression
significantly
driven
by
cardiac
fibrosis,
pathological
process
extracellular
matrix
undergoes
abnormal
uncontrolled
remodelling.
Cardiac
fibrosis
characterized
excessive
protein
deposition
activation
myofibroblasts,
increasing
stiffness
heart,
thus
disrupting
its
normal
structure
function
promoting
lethal
arrythmia.
MicroRNAs,
long
non-coding
RNAs,
circular
collectively
known
as
RNAs
(ncRNAs),
have
recently
gained
significant
attention
due
growing
body
evidence
suggesting
their
involvement
remodelling
such
fibrosis.
ncRNAs
found
peripheral
blood,
indicating
potential
biomarkers
for
assessing
In
this
review,
we
critically
examine
recent
advancements
findings
related
use
discuss
implication
development.
Язык: Английский
DNA Molecular Computing with Weighted Signal Amplification for Cancer miRNA Biomarker Diagnostics
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 11, 2025
Abstract
The
expression
levels
of
microRNAs
(miRNAs)
are
strongly
linked
to
cancer
progression,
making
them
promising
biomarkers
for
detection.
Enzyme‐free
signal
amplification
DNA
circuits
have
facilitated
the
detection
low‐abundance
miRNAs.
However,
these
methods
may
neglect
diagnostic
value
(or
weight)
different
Here,
a
molecular
computing
approach
with
weighted
is
presented.
Polymerase‐mediated
strand
displacement
employed
assign
weights
target
miRNAs,
reflecting
miRNAs’
values,
followed
by
signals
using
localized
catalytic
hairpin
assembly.
This
method
applied
diagnose
miRNAs
non‐small
cell
lung
(NSCLC).
Machine
learning
used
identify
NSCLC‐specific
and
corresponding
optimum
classification
healthy
individuals.
With
output
simplified
as
single
channel
fluorescence
intensity.
Cancer
tissues
(
n
=
18)
adjacent
10)
successfully
classified
within
2.5
h
(sample‐to‐result)
an
accuracy
92.86%.
strategy
has
potential
extend
digital
multidimensional
biomarkers,
advancing
personalized
disease
diagnostics
in
point‐of‐care
settings.
Язык: Английский
MicroRNA Nobel Prize: Timely Recognition and High Anticipation of Future Products—A Prospective Analysis
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(23), С. 12883 - 12883
Опубликована: Ноя. 29, 2024
MicroRNAs
(miRNAs)
maintain
cellular
homeostasis
by
blocking
mRNAs
binding
with
them
to
fine-tune
the
expression
of
genes
across
numerous
biological
pathways.
The
2024
Nobel
Prize
in
Medicine
and
Physiology
for
discovering
miRNAs
was
long
overdue.
We
anticipate
a
deluge
research
work
involving
repeat
history
prizes
awarded
on
other
RNAs.
Although
miRNA
therapies
are
included
several
complex
diseases,
realization
that
regulate
their
roles
addressing
hundreds
diseases
expected;
but
advancement
drug
discovery
tools,
we
even
faster
entry
new
drugs.
To
promote
this,
provide
details
current
science,
logic,
intellectual
property,
formulations,
regulatory
process
anticipation
many
more
researchers
will
introduce
novel
based
discussion
advice
provided
this
paper.
Язык: Английский
The circulatory levels and bone expression of MIR21, 34a, 155 and their target genes in a section of Egyptian Population
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Ноя. 13, 2024
Abstract
Bone
tissue
is
constantly
regenerated
and
repaired
through
a
finely
balanced
process
known
as
bone
remodeling.
Many
miRNAs
act
regulators
of
the
signaling
pathways
involved
in
metabolic
processes
to
maintain
homeostasis.
This
study
aimed
assess
circulating
levels
MIR21,
MIR34a,
MIR155
human
serum
their
expression,
expression
turnover-related
genes
which
can
reflect
quality.
prospective
was
conducted
on
60
patients
(30
males
30
females)
indicated
for
surgical
interventions
neural
decompression
+/-
fixation.
Relative
quantification
miR34a,
related
assayed
using
PCR.
The
osteocalcin
Serum
Alkaline
Phosphatase
(sBAP)
were
ELISA
kit.
main
finding
present
work
strong
positive
association
between
only
miR21
population
under
with
markers
target
Also,
found
both
MIR21
age
sBAP.
These
results
suggest
that
these
microRNAs
early
predication
Язык: Английский