Deciphering the evolution and complexity of wheat germplasm from a genomic perspective DOI Creative Commons
Zihao Wang, Ling-Feng Miao, Yongming Chen

и другие.

Journal of genetics and genomics/Journal of Genetics and Genomics, Год журнала: 2023, Номер 50(11), С. 846 - 860

Опубликована: Авг. 21, 2023

Bread wheat provides an essential fraction of the daily calorific intake for humanity. Due to its huge and complex genome, progresses in studying on genome are substantially trailed behind those other two major crops, rice maize, at least a decade. With rapid advances assembling reduced cost high-throughput sequencing, emerging de novo assemblies whole-genome sequencing data leading paradigm shift research. Here, we review recent progress dissecting germplasm evolution since release first high-quality genome. New insights have been gained during domestication modern breeding progress, genomic variations multiple scales contributing diversity germplasm, transcriptional epigenetic regulations functional genes polyploid wheat. Genomics databases bioinformatics tools meeting urgent needs genomics research also summarized. The ever-increasing omics data, along with advanced well-structured databases, expected accelerate deciphering gene resources future advances.

Язык: Английский

A kinase fusion protein from Aegilops longissima confers resistance to wheat powdery mildew DOI Creative Commons
Huagang He, Zhaozhao Chen,

Renchun Fan

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Авг. 2, 2024

Many disease resistance genes have been introgressed into wheat from its wild relatives. However, reduced recombination within the segments hinders cloning of genes. Here, we cloned powdery mildew gene Pm13, which is Aegilops longissima, using a method that combines physical mapping with radiation-induced chromosomal aberrations and transcriptome sequencing analysis ethyl methanesulfonate (EMS)-induced loss-of-function mutants. Pm13 encodes kinase fusion protein, designated MLKL-K, an N-terminal domain mixed lineage domain-like protein (MLKL_NTD domain) C-terminal serine/threonine bridged by brace. The function validated through transient stable transgenic complementation assays. Transient over-expression analyses in Nicotiana benthamiana leaves protoplasts reveal fragment Brace-Kinase122-476 MLKL-K capable inducing cell death, dependent on functional three α-helices brace region close to N-terminus domain. was longissima. authors report it MLKL_NTD domain-containing kinase.

Язык: Английский

Процитировано

8
Multi-omics analyzes of Rosa gigantea illuminate tea scent biosynthesis and release mechanisms DOI Creative Commons
Lijun Zhou,

Sihui Wu,

Yunyi Chen

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Сен. 30, 2024

Rose is an important ornamental crop cultivated globally for perfume production. However, our understanding of the mechanisms underlying scent production and molecular breeding fragrance hindered by lack a reference genome tea roses. We present first complete telomere-to-telomere (T2T) Rosa gigantea, with high quality (QV > 60), including detailed characterization structural features repetitive regions. The expansion genes associated phenylpropanoid biosynthesis may account unique scent. uncover release rhythm aromatic volatile organic compounds their gene regulatory networks through comparative genomics time-ordered co-expression networks. Analyzes eugenol homologs demonstrate how plants attract pollinators using specialized phenylpropanoids in specific tissues. This study highlights conservation utilization genetic diversity from wild endangered species multi-omics approaches, providing scientific foundation enhancing rose via de novo domestication.

Язык: Английский

Процитировано

8
CRISPR/Cas9‐targeted mutagenesis of TaDCL4, TaDCL5 and TaRDR6 induces male sterility in common wheat DOI Creative Commons
Rongzhi Zhang, Shujuan Zhang,

Jihu Li

и другие.

Plant Biotechnology Journal, Год журнала: 2023, Номер 21(4), С. 839 - 853

Опубликована: Янв. 4, 2023

Summary Phased, small interfering RNAs (phasiRNAs) are important for plant anther development, especially male sterility. PhasiRNA biogenesis is dependent on genes like RNA polymerase 6 (RDR6), DICER‐LIKE 4 (DCL4), or DCL5 to produce 21‐ 24 nucleotide (nt) double‐strand RNAs. Here, we generated mutants of DCL4 , and RDR6 using CRISPR/Cas9 system studied their effects reproductive development phasiRNA production in wheat. We found that mutation caused sever consequence throughout starting from seed germination the dcl4 grew weaker with thorough sterility, while dcl5 plants developed normally but exhibited Correspondingly, DCL5, respectively, specified 24‐nt biogenesis, contributed both. Also, three key evolved differently wheat, TaDCL5‐A/B becoming non‐functioning TaRDR6‐A being lost after polyploidization. Furthermore, PHAS (phasiRNA precursors) identified via phasiRNAs diverged rapidly among sub‐genomes polyploid Despite no similarity grasses, targets were enriched similar biological functions. In light roles pathways gametophyte genetic dissection function may help generate sterile lines suitable hybrid wheat breeding.

Язык: Английский

Процитировано

17
Genome sequencing of Sitopsis species provides insights into their contribution to the B subgenome of bread wheat DOI Creative Commons
Yuxin Yang, Licao Cui, Zefu Lu

и другие.

Plant Communications, Год журнала: 2023, Номер 4(4), С. 100567 - 100567

Опубликована: Фев. 28, 2023

Wheat (Triticum aestivum, BBAADD) is an allohexaploid species that originated from two polyploidization events. The progenitors of the A and D subgenomes have been identified as Triticum urartu Aegilops tauschii, respectively. Current research suggests speltoides closest but not direct ancestor B subgenome. However, whether Ae. has contributed genomically to wheat subgenome which chromosome regions are conserved between remain unclear. Here, we assembled a high-quality reference genome for speltoides, resequenced 53 accessions seven (Aegilops bicornis, longissima, searsii, sharonensis, mutica [syn. Amblyopyrum muticum], dicoccoides) revealed their genomic contributions Our results showed centromeric were particularly 0.17 Gb blocks We classified five groups non-conserved genes Triticum, revealing biological characteristics, differentiation in gene expression patterns, collinear relationships also families expanded during its evolution 789 specific speltoides. These can serve genetic resources improvement adaptability biotic abiotic stress. newly constructed large-scale resequencing data Sitopsis will provide valuable resource studies.

Язык: Английский

Процитировано

17
Deciphering the evolution and complexity of wheat germplasm from a genomic perspective DOI Creative Commons
Zihao Wang, Ling-Feng Miao, Yongming Chen

и другие.

Journal of genetics and genomics/Journal of Genetics and Genomics, Год журнала: 2023, Номер 50(11), С. 846 - 860

Опубликована: Авг. 21, 2023

Bread wheat provides an essential fraction of the daily calorific intake for humanity. Due to its huge and complex genome, progresses in studying on genome are substantially trailed behind those other two major crops, rice maize, at least a decade. With rapid advances assembling reduced cost high-throughput sequencing, emerging de novo assemblies whole-genome sequencing data leading paradigm shift research. Here, we review recent progress dissecting germplasm evolution since release first high-quality genome. New insights have been gained during domestication modern breeding progress, genomic variations multiple scales contributing diversity germplasm, transcriptional epigenetic regulations functional genes polyploid wheat. Genomics databases bioinformatics tools meeting urgent needs genomics research also summarized. The ever-increasing omics data, along with advanced well-structured databases, expected accelerate deciphering gene resources future advances.

Язык: Английский

Процитировано

14