bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 17, 2024
Abstract
Salvia
species
native
to
the
Americas
are
rich
in
valuable
bioactive
furanoclerodanes,
like
psychoactive
salvinorin
A
found
divinorum
,
which
is
used
treatment
of
opioid
addiction.
However,
there
relatively
little
known
about
their
biosynthesis.
To
address
this,
we
investigated
biosynthesis
salviarin,
simplest
furanoclerodane
structure
ornamental
sage
splendens
.
Using
a
self-organizing
map
and
mutual
rank
analysis
RNA-seq
co-expression
data,
identified
three
cytochrome
P450
enzymes
responsible
for
converting
kolavenol
into
salviarin
precursors,
consecutively:
annonene,
hardwickiic
acid
hautriwaic
acid.
As
annonene
have
also
been
proposed
as
intermediates
A,
examine
our
hypothesis
common
evolutionary
origin
pathway
between
two
species,
searched
homologous
genes
available
data
S.
The
encoded
by
orthologous
from
displayed
synthase
(SdKLS),
(SdANS),
(SdHDAS)
activity
respectively,
supporting
view
that
these
intermediate
steps
A.
We
role
gene
duplication
evolution
this
specific
activity.
Our
work
shows
how
can
serve
model
studying
furanoclerodanes
contributes
understanding
specialized
metabolism
plants,
provides
new
tools
produce
biotechnological
chassis.
Science of Traditional Chinese Medicine,
Год журнала:
2023,
Номер
1(1), С. 26 - 34
Опубликована: Сен. 1, 2023
Abstract
The
Nobel
Prize
for
artemisinin
and
the
COVID-19
pandemic
have
heightened
interest
in
disease
prevention
treatment
of
traditional
Chinese
medicine
(TCM).
However,
uncertainty
misinformation
remain
about
some
key
issues.
Herein,
we
discuss
current
literature
showing
that,
despite
TCM
gaining
recognition,
theoretical
systems
Western
rooted
different
cultural
backgrounds
huge
differences
their
theories
treatments.
How
to
achieve
accurate
diagnosis
TCM,
how
give
scientific
treatment,
what
is
mechanism
are
effect
safety
all
need
be
more
clearly
explained
require
high-quality
modern
evidence.
An
emerging
consensus
exists
with
help
technology
methods,
it
necessary
excavate
essence
that
truly
conforms
laws
human
life
movement
development,
realize
cross-complementation
medicine,
enhance
understanding
diseases,
enrich
promote
a
new
level
field
sciences.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 29, 2024
Summary
Hyperforin
is
the
compound
responsible
for
effectiveness
of
St.
John’s
wort
(
Hypericum
perforatum
)
as
an
antidepressant,
but
its
biosynthesis
remains
unknown.
Gene
discovery
based
on
co-expression
analysis
bulk
RNA-sequencing
data
or
genome
mining
failed
to
discover
missing
steps
in
hyperforin
biosynthesis.
Here
we
sequenced
tetraploid
H.
genome.
By
single-cell
RNA-seq,
identified
a
distinct
type
cells,
Hyper
wherein
de
novo
takes
place.
Through
pathway
reconstitution
yeast
and
tobacco,
identify
characterize
four
transmembrane
prenyltransferases
resolve
The
polycyclic
scaffold
created
by
reaction
cascade
involving
irregular
isoprenoid
coupling
tandem
cyclization.
Our
findings
reveal
how
where
biosynthesized
that
enables
synthetic-biology
complete
pathway.
These
results
deepen
our
comprehension
specialized
metabolism
at
cellular
level,
anticipate
acceleration
elucidation
plant
metabolism.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 29, 2024
SUMMARY
The
Lamiaceae
(mint
family)
is
the
largest
known
source
of
furanoclerodanes,
a
subset
clerodane
diterpenoids
with
broad
bioactivities
including
insect
antifeedant
properties.
Ajugoideae
subfamily,
in
particular,
accumulates
significant
numbers
structurally
related
furanoclerodanes.
biosynthetic
capacity
for
formation
these
retained
across
most
subfamilies,
early-diverging
Callicarpoideae
which
forms
sister
clade
to
rest
Lamiaceae.
VacCYP76BK1
,
cytochrome
P450
monooxygenase
from
Vitex
agnus-castus
was
previously
found
catalyze
proposed
precursor
furan
and
lactone-containing
labdane
diterpenoids.
Through
transcriptome-guided
pathway
exploration,
we
identified
orthologs
Ajuga
reptans
Callicarpa
americana.
Functional
characterization
demonstrated
that
both
could
oxidative
cyclization
backbones
yield
ring.
Subsequent
investigation
revealed
total
ten
CYP76BK1
six
subfamilies.
analysis
available
chromosome-scale
genomes,
four
members
as
syntelogs
within
conserved
syntenic
block
divergent
This
suggests
an
evolutionary
lineage
predates
speciation
affirmed
conservation
function,
all
catalyzed
ring
formation.
Additionally,
some
yielded
two
novel
lactone
moieties.
presence
subfamilies
closely
overlaps
distribution
reported
Together,
activities
here
support
their
central
role
furanoclerodane
biosynthesis
family.
Our
findings
lay
groundwork
biotechnological
applications
harness
economic
potential
this
promising
class
compounds.
Significance
Statement
discovery
functional
diverse
chemistry
biosynthesis,
providing
insights
into
metabolic
landscape
dynamic
evolution
plant
family
over
approximately
50
million
years.
These
pave
way
targeted
engineering
efforts
sustainable
production
compounds,
offering
prospects
agricultural
pharmaceutical
applications.
The Plant Journal,
Год журнала:
2024,
Номер
120(3), С. 984 - 997
Опубликована: Сен. 14, 2024
SUMMARY
The
Lamiaceae
(mint
family)
is
the
largest
known
source
of
furanoclerodanes,
a
subset
clerodane
diterpenoids
with
broad
bioactivities
including
insect
antifeedant
properties.
Ajugoideae
subfamily,
in
particular,
accumulates
significant
numbers
structurally
related
furanoclerodanes.
biosynthetic
capacity
for
formation
these
retained
across
most
subfamilies,
early‐diverging
Callicarpoideae
which
forms
sister
clade
to
rest
Lamiaceae.
VacCYP76BK1
,
cytochrome
P450
monooxygenase
from
Vitex
agnus‐castus
was
previously
found
catalyze
proposed
precursor
furan
and
lactone‐containing
labdane
diterpenoids.
Through
transcriptome‐guided
pathway
exploration,
we
identified
orthologs
Ajuga
reptans
Callicarpa
americana.
Functional
characterization
demonstrated
that
both
could
oxidative
cyclization
backbones
yield
ring.
Subsequent
investigation
revealed
total
10
CYP76BK1
six
subfamilies.
analysis
available
chromosome‐scale
genomes,
four
members
as
syntelogs
within
conserved
syntenic
block
divergent
This
suggests
an
evolutionary
lineage
predates
speciation
affirmed
conservation
function,
all
catalyzed
ring
formation.
Additionally,
some
yielded
two
novel
lactone
moieties.
presence
subfamilies
closely
overlaps
distribution
reported
Together,
activities
here
support
their
central
role
furanoclerodane
biosynthesis
family.
Our
findings
lay
groundwork
biotechnological
applications
harness
economic
potential
this
promising
class
compounds.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 17, 2024
Abstract
Salvia
species
native
to
the
Americas
are
rich
in
valuable
bioactive
furanoclerodanes,
like
psychoactive
salvinorin
A
found
divinorum
,
which
is
used
treatment
of
opioid
addiction.
However,
there
relatively
little
known
about
their
biosynthesis.
To
address
this,
we
investigated
biosynthesis
salviarin,
simplest
furanoclerodane
structure
ornamental
sage
splendens
.
Using
a
self-organizing
map
and
mutual
rank
analysis
RNA-seq
co-expression
data,
identified
three
cytochrome
P450
enzymes
responsible
for
converting
kolavenol
into
salviarin
precursors,
consecutively:
annonene,
hardwickiic
acid
hautriwaic
acid.
As
annonene
have
also
been
proposed
as
intermediates
A,
examine
our
hypothesis
common
evolutionary
origin
pathway
between
two
species,
searched
homologous
genes
available
data
S.
The
encoded
by
orthologous
from
displayed
synthase
(SdKLS),
(SdANS),
(SdHDAS)
activity
respectively,
supporting
view
that
these
intermediate
steps
A.
We
role
gene
duplication
evolution
this
specific
activity.
Our
work
shows
how
can
serve
model
studying
furanoclerodanes
contributes
understanding
specialized
metabolism
plants,
provides
new
tools
produce
biotechnological
chassis.