Arabidopsis CIRP1 E3 ligase modulates drought and oxidative stress tolerance and reactive oxygen species homeostasis by directly degrading catalases

Journal of Integrative Plant Biology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 28, 2025

ABSTRACT Reactive oxygen species (ROS) plays critical roles in modulating plant growth and stress response its homeostasis is fine tuned using multiple peroxidases. H 2 O , a major kind of ROS, removed rapidly directly three catalases, CAT1, CAT2, CAT3, Arabidopsis. Although the activity regulations catalases have been well studied, their degradation pathway less clear. Here, we report that CAT2 CAT3 protein abundance was partially controlled 26S proteasome. To further identify candidate proteins modulate stability performed yeast‐two‐hybrid screening recovered several clones encoding with RING vWA domains, CIRP1 ( C AT2 I nteracting R ING P rotein 1 ). Drought oxidative downregulated transcripts. harbored E3 ubiquitination accelerated by direct interaction ubiquitination. The cirp1 mutants exhibited stronger drought tolerance, which opposite to cat2 cat3 mutants. Genetic analysis revealed acts upstream negatively regulate tolerance. increased tolerance due enhanced catalase (CAT) activities alleviated ROS levels. Our data CIRP1–CAT2/CAT3 module vital role alleviating levels balancing responses

Язык: Английский

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Advancements in hybrid rice production: improvements in male sterility and synthetic apomixis for sustainable agriculture

Plant Biotechnology Journal, Год журнала: 2025, Номер unknown

Опубликована: Март 20, 2025

Rice serves as a staple food for approximately half of the world's population, and enhanced yields from hybrid rice play crucial role in ensuring security augmenting incomes. However, annual purchase high cost seeds hinder widespread adoption. In this review, we discuss seed production strategies based on molecular mechanisms along with biotechnological techniques employed future prospects. Male-sterile lines are pivotal production, ongoing developments markedly advancing process. Initially, cytoplasmic male-sterile facilitated three-line production. Subsequent innovations, including environmentally responsive gene-based biotechnology-driven lines, enabled two-line Ongoing research is focusing implementing one-line method using apomixis, driving innovation Overall, advancements synthetic apomixis present promising avenues improving efficiency sustainability These highlight critical need continued concerted efforts to address global challenges.

Язык: Английский

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The F box protein INHIBITOR FOR BROWN FURROWS 1 (IBF1) regulates flavonoid accumulation in rice hull by promoting degradation of chalcone synthase 1

The Plant Journal, Год журнала: 2025, Номер 121(6)

Опубликована: Март 1, 2025

SUMMARY Flavonoids are secondary metabolites of plants that play various roles in plants. The transcriptional level regulation flavonoid synthesis has been extensively studied, but research on the protein is still limited. In present study, a brown hull mutant, bh2 , was screened from an ethane methyl sulfonate (EMS)‐induced bank seeds indica cultivar RH2B. mutant exhibited phenotype and higher levels total flavonoids anthocyanins compared with wild‐type We identified gene INHIBITOR FOR BROWN FURROWS 1 ( IBF1 ) through MutMap analysis subsequently cloned it. encodes F‐box involved formation SCF (S‐phase kinase‐associated [SKP1], Cullin, F‐box) complex Oryza sativa SKP1‐like proteins OSK1/OSK20. Through yeast two‐hybrid, bimolecular fluorescence complementation, pull‐down assays, interaction chalcone synthase (CHS1) confirmed. This facilitated degradation CHS1 ubiquitin‐26S proteasome system. ibf1 chs1 double mutants normal color, restoring . Genetic suggested regulates color CHS1‐dependent manner. Collectively, our study suggests serves as crucial negative regulator controls biosynthesis by mediating degradation, thereby regulating color.

Язык: Английский

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OsNRAMP7 positively regulates heat tolerance at seedling and reproductive stages in rice

Plant Stress, Год журнала: 2025, Номер unknown, С. 100870 - 100870

Опубликована: Апрель 1, 2025

Язык: Английский

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The mutation in the critical sterility-inducing temperature-regulating gene OsTMS5 and OsCSIT1 enhances cadmium tolerance in rice seedlings

Plant Growth Regulation, Год журнала: 2025, Номер unknown

Опубликована: Апрель 28, 2025

Язык: Английский

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PuUBL5-mediated ZINC FINGER PROTEIN 1 stability is critical for root development under drought stress in Populus ussuriensis

PLANT PHYSIOLOGY, Год журнала: 2025, Номер 198(1)

Опубликована: Апрель 30, 2025

Abstract C2H2-type zinc finger protein (ZFP) transcription factors influence root growth and development. However, their potential roles in inhibiting adventitious (AR) lateral (LR) formation trees remain unclear. Here, we report that the ABA-responsive factor (PuZFP1) regulates Populus ussuriensis development to enhance drought tolerance. PuZFP1 negatively LR by binding PuWRKY46 promoter its expression. At same time, promotes AR elongation repressing Clade E Growth-Regulating (EGR) Type 2C phosphatases (PuEGR1). In PuZFP1-overexpressing lines, a higher ABA/IAA ratio differentiated zone (DZ) drives PuWRKY46-mediated inhibition. Conversely, lower is associated with expression of downstream target gene PuEGR1 (EZ). Notably, physically interacts Ubiquitin-like 5 (PuUBL5) undergoes 26S proteasome-mediated degradation. Taken together, our findings shed light on role PuUBL5–PuZFP1 module mediating crosstalk between emergence via ABA/auxin signaling drought-stressed P. ussuriensis, provide insights into regulatory network underlying PuZFP1-mediated poplar.

Язык: Английский

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GhTKPR1_8 functions to inhibit anther dehiscence and reduce pollen viability in cotton

Physiologia Plantarum, Год журнала: 2024, Номер 176(3)

Опубликована: Май 1, 2024

Abstract Sporopollenin, as the main component of pollen exine, is a highly resistant polymer that provides structural integrity under unfavourable environmental conditions. Tetraketone α‐pyrone reductase 1 (TKPR1) essential for sporopollenin formation, catalyzing reduction tetraketone carbonyl to hydroxylated α‐pyrone. The functional role TKPR1 in male sterility has been reported flowering plants such maize, rice, and Arabidopsis . However, molecular cloning characterization cotton remain unaddressed. In this study, we identified 68 TKPR1s from four species, categorized into three clades. Transcriptomics RT‐qPCR demonstrated GhTKPR1_8 exhibited typical expression patterns tetrad stage anther. was localized endoplasmic reticulum. Moreover, ABORTED MICROSPORES (GhAMS) transcriptionally activated indicated by luciferase complementation tests. ‐knockdown inhibited anther dehiscence reduced viability cotton. Additionally, overexpression attkpr1 mutant restored its sterile phenotype. This study offers novel insights investigation while providing genetic resources studying sterility.

Язык: Английский

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Exploiting male sterility toward the development of hybrid rice

Seed Biology, Год журнала: 2024, Номер 3(1), С. 0 - 0

Опубликована: Янв. 1, 2024

Язык: Английский

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RIP5 Interacts with REL1 and Negatively Regulates Drought Tolerance in Rice

Cells, Год журнала: 2024, Номер 13(11), С. 887 - 887

Опубликована: Май 21, 2024

Improving the drought resistance of rice is great significance for expanding planting area and improving stable yield rice. In our previous work, we found that ROLLED AND ERECT LEAF1 (REL1) protein promoted enhanced tolerance to stress by eliminating reactive oxygen species (ROS) levels triggering abscisic acid (ABA) response. However, mechanism through which REL1 regulates removing ROS unclear. this study, identified interacting 5 (RIP5) it directly combines with in chloroplast. We RIP5 was strongly expressed ZH11 under drought-stress conditions, rip5-ko mutants significantly improved plants drought, whereas overexpression resulted greater susceptibility drought. Further investigation suggested negatively regulated decreasing content ascorbic (AsA), thereby reducing clearance. RNA sequencing showed knockout caused differential gene expression chiefly associated ascorbate aldarate metabolism. Furthermore, multiple experimental results suggest involved regulating inhibiting RIP5. Collectively, findings reveal importance inhibition affecting rice’s response stress. This work not only explains rice, but will also help improve

Язык: Английский

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The Residual Activity of Fatty Acyl‐CoA Reductase Underlies Thermo‐Sensitive Genic Male Sterility in Rice

Plant Cell & Environment, Год журнала: 2024, Номер unknown

Опубликована: Окт. 23, 2024

ABSTRACT Photoperiod/thermo‐sensitive genic male sterility (P/TGMS) is critical for rice two‐line hybrid system. Previous studies showed that slow development of pollen a general mechanism sterility‐to‐fertility conversion TGMS in Arabidopsis. However, whether this still exists unknown. Here, we identified novel line, ostms16 , which exhibits abnormal exine under high temperature and fertility restoration low temperature. In mutant, single base mutation OsTMS16 fatty acyl‐CoA reductase (FAR), reduced its enzyme activity, leading to defective wall. Under temperature, the mOsTMS16 M549I couldn't provide sufficient protection microspores. activity closer OsTMS16, so imperfect could protect microspore development. These results indicated residual mutant meet requirement different determinant factor P/TGMS lines. Additionally, previously found res2 polygalacturonase tetrad pectin wall degradation, restored multiple lines study, proved osres2 indicating also suitable rice.

Язык: Английский

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