Functions and Regulation of HAM Family Genes in Meristems During Gametophyte and Sporophyte Generations DOI Creative Commons
Yuan Geng,

Chong Xie,

Cankui Zhang

и другие.

Plant Cell & Environment, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 18, 2024

ABSTRACT A fascinating feature of land plants is their ability to continually initiate new tissues and organs throughout lifespan, driven by a pool pluripotent stem cells located in meristems. In seed plants, various types meristems are initiated maintained during the sporophyte generation, while gametophytes lack rely on for growth. contrast, seed‐free vascular such as ferns, develop both gametophyte generations, allowing independent growth generations. Recent findings have highlighted conserved lineage‐specific roles HAIRY MERISTEM (HAM) family GRAS‐domain transcriptional regulators plant lifecycle. Here, we review discuss how HAM genes maintain meristem indeterminacy sporophytes gametophytes, with focus studies performed two model species: flowering Arabidopsis thaliana fern Ceratopteris richardii . Additionally, summarize crucial tightly regulated functions microRNA171 (miR171)‐HAM regulatory modules, which define spatial patterns activities development across identities plants.

Язык: Английский

Sucrose homeostasis: Mechanisms and opportunity in crop yield improvement DOI Creative Commons
Javier A. Miret, Cara A. Griffiths, Matthew J. Paul

и другие.

Journal of Plant Physiology, Год журнала: 2024, Номер 294, С. 154188 - 154188

Опубликована: Янв. 29, 2024

Sugar homeostasis is a critical feature of biological systems. In humans, raised and dysregulated blood sugar serious health issue. plants, directed changes in sucrose allocation represent opportunities crop improvement. Plant tissue varies more than glucose found at higher concentrations (cytosol phloem ca. 100 mM v 3.9-6.9 for glucose). Tissue with developmental stage environment, but cytosol exhibit tight control. Sucrose consequence the integration photosynthesis, synthesis storage end-products such as starch, transport to sinks sink metabolism. Trehalose 6-phosphate (T6P)-SnRK1 TOR play central, still emerging roles regulating coordinating these processes. Overall, levels are strongly related growth photosynthesis. As key signal, T6P regulates levels, metabolic pathways coordinate source whole plant level. Emerging evidence shows that interacts meristems. With careful targeting, manipulation through exploiting natural variation, chemical intervention genetic modification delivering benefits yields. Regulation cereal grain set, filling retention may be most strategically important aspect food security.

Язык: Английский

Процитировано

11
Large-scale single-cell profiling of stem cells uncovers redundant regulators of shoot development and yield trait variation DOI Open Access
Xiaosa Xu, Michael J. Passalacqua, Brian R. Rice

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 6, 2024

SUMMARY Stem cells in plant shoots are a rare population of that produce leaves, fruits and seeds, vital sources for food bioethanol. Uncovering regulators expressed these stem will inform crop engineering to boost productivity. Single-cell analysis is powerful tool identifying specific groups cells. However, accessing shoot challenging. Recent single-cell analyses have not captured cells, failed detect cell like CLAVATA3 WUSCHEL . In this study, we finely dissected cell-enriched tissues from both maize arabidopsis RNA-seq profiling. We optimized protocols efficiently recover thousands A cross-species comparison identified conserved between arabidopsis. also performed on overproliferation mutants find additional candidate regulators. Expression genes was validated using spatial transcriptomics, functionally confirmed roles development. These candidates include family ribosome-associated RNA-binding proteins, two families sugar kinase related hypoxia signaling cytokinin hormone homeostasis. large-scale profiling provide resource mining regulators, which show significant association with yield traits. Overall, our discoveries advance the understanding development open avenues manipulating diverse crops enhance energy security.

Язык: Английский

Процитировано

6
The Central Role of Auxin in Orchestrating Apical Stem Cells in Plants DOI Open Access

Jun Ma,

Yanan Wang, Shuo Chen

и другие.

Plant Cell & Environment, Год журнала: 2025, Номер unknown

Опубликована: Март 28, 2025

ABSTRACT Plant stem cells, residing in the shoot and root apical meristems, are fundamental for continuous growth organ formation throughout plant life cycle. Their regulation is driven by convergence of endogenous developmental cues exogenous environmental signals, making them pivotal to overall development. Auxin, a key phytohormone, serves as major internal signal, orchestrating cell initiation, maintenance, differentiation, adaptation through intricate biosynthesis, transport, signaling networks. This review summarizes recent progress understanding cellular molecular mechanisms which auxin guides functions both meristems. Through these insights, we explore how plants utilize auxin‐driven pathways optimize ever‐changing environments.

Язык: Английский

Процитировано

0
A plant CLE peptide and its fungal mimic promote arbuscular mycorrhizal symbiosis via CRN-mediated ROS suppression DOI Creative Commons
Sagar Bashyal,

Hasani Everett,

S. Matsuura

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2025, Номер 122(16)

Опубликована: Апрель 14, 2025

CLAVATA3/EMBRYO SURROUNDING REGION-related (CLE) peptides have emerged as key regulators of plant–microbe interactions, including arbuscular mycorrhizal (AM) symbiosis. Here, we identify Medicago truncatula CLE16 a positive regulator AM MtCLE16 is expressed in root cells colonized by fungi (AMF) and its overexpression within tissues increases arbuscule abundance finetuning their growth lifespan. Functional transcriptomic analyses reveal that acts via the M. pseudokinase CORYNE (MtCRN) suppresses accumulation reactive oxygen species (ROS) roots, thereby attenuating immune responses promoting colonization mutualistic fungi. Notably, AMF also express MtCLE16-like peptides. We show Rhizophagus irregularis peptide, RiCLE1, attenuates ROS promotes MtCRN. This finding suggests RiCLE1 can interfere with MtCLE16-MtCRN signaling module host roots to benefit fungus. Our research uncovers functional mechanism underpinning cross-kingdom molecular mimicry interactions.

Язык: Английский

Процитировано

0
Integrative approaches to enhance reproductive resilience of crops for climate-proof agriculture DOI Creative Commons

Collins Agho,

Adi Avni,

Ariola Bacu

и другие.

Plant Stress, Год журнала: 2024, Номер unknown, С. 100704 - 100704

Опубликована: Дек. 1, 2024

Язык: Английский

Процитировано

3
Medicago truncatula CORYNE regulates inflorescence meristem branching, nutrient signaling, and arbuscular mycorrhizal symbiosis DOI Creative Commons

Johnathan Orosz,

Erica Xinlei Lin,

Penelope L. Lindsay

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 23, 2024

Abstract The CLAVATA signaling pathway regulates plant development and plant-environment interactions. consists of mobile, cell-type or environment-specific CLAVATA3/ESR-related (CLE) peptides, which are perceived by a receptor complex consisting leucine-rich repeat receptor-like kinases such as CLAVATA1 proteins CLAVATA2, often functions with the pseudokinase CORYNE (CRN). has been extensively studied in various species for its role meristem maintenance legumes modulating root interactions nitrogen-fixing rhizobia. Some involved nodulation, including CLAVATA1, also regulate mutualistic arbuscular mycorrhizal (AM) fungi. However, our knowledge on AM symbiosis regulation remains limited only handful genetic regulators have identified. Here we report that Medicago truncatula CRN controls inflorescence branching negatively MtCRN partially independently autoregulation signal MtCLE53 . Transcriptomic data revealed crn roots display signs perturbed related to range responses biotic abiotic factors, suggesting plays roles environment.

Язык: Английский

Процитировано

2
Identification and Expression Analysis of the WOX Transcription Factor Family in Foxtail Millet (Setaria italica L.) DOI Open Access

Lizhang Nan,

Yajun Li,

Cui Ma

и другие.

Genes, Год журнала: 2024, Номер 15(4), С. 476 - 476

Опубликована: Апрель 10, 2024

WUSCHEL-related homeobox (WOX) transcription factors are unique to plants and play pivotal roles in plant development stress responses. In this investigation, we acquired protein sequences of foxtail millet WOX gene family members through homologous sequence alignment a hidden Markov model (HMM) search. Utilizing conserved domain prediction, identified 13 genes, which were classified into ancient, intermediate, modern clades. Multiple results revealed that all proteins possess homeodomain (HD). The SiWOX clustered together the phylogenetic tree, exhibited analogous spatial structures, motifs. genes distributed across 7 chromosomes, featuring 3 pairs tandem repeats: SiWOX1 SiWOX13, SiWOX4 SiWOX5, SiWOX11 SiWOX12. Collinearity analysis demonstrated exhibit highest collinearity with green foxtail, followed by maize. primarily harbor two categories cis-acting regulatory elements: Stress response hormone response. Notably, prominent hormones triggering responses include methyl jasmonate, abscisic acid, gibberellin, auxin, salicylic acid. Analysis expression patterns unveiled potential functional diversity among different millet. These findings lay solid foundation for further elucidating functions evolution genes.

Язык: Английский

Процитировано

1
A bushel of WUSCHEL is too much: Uncovering the role of cytokinin cis-regulatory elements in the maize WUSCHEL promoter DOI Creative Commons
Kyle W. Swentowsky

PLANT PHYSIOLOGY, Год журнала: 2024, Номер 194(4), С. 2183 - 2184

Опубликована: Янв. 12, 2024

Plants are shaped by structures called meristems that contain stem cells.The shoot apical meristem (SAM) produces above-ground vegetative and reproductive organs, including many harvested for agricultural production.Studies in plants such as maize, tomato, rice have demonstrated manipulating the size shape of through genetics is an effective method to improve crops.The most important SAM signaling pathway a wellstudied feedback loop between WUSCHEL (WUS) transcription factor CLAVATA3/Embryo Surrounding Region-Related (CLE) peptides (Fig. 1A; reviewed Lindsay et al. 2024).WUS expressed cells within at organizing center secreted epidermal layer, where it activates expression CLV3.The CLV3 peptide travels center, limits WUS expression.Manipulating CLV-WUS components alters or organization usually results changed final plant structure.This was first discovered Arabidopsis but functions similarly other plants, crop species.Although originally described SAM, module also shapes inflorescence meristems.Maize lacking functional CLE complete disorganization meristem, resulting highly malformed ears.However, when this only moderately reduced using CRISPR-Cas9 promoter editing, ears were just slightly disorganized contained more kernels (Liu 2021).This demonstrates how genome engineering genes could lead innovations improvement.In issue Plant Physiology, Chen ( 2023) investigated gene taking advantage naturally occurring maize mutant Barren inflorescence3 (Bif3) has small, ball-shaped with few kernels.The Bif3 phenotype caused tandem duplication homolog, ZmWUS1.The additional copy (ZmWUS1-B) causes overexpression excessive proliferation (Chen 2021).The authors used comparative genomics approach analyze conservation its promoter.Besides conserved body, 84-bp enhancer sequence Panicoideae subfamily.Within lies single AGATAT motif canonical binding site type-B ARABIDOPSIS RESPONSE REGULATORs control response cytokinin signaling.This necessary initiate (Zhang 2017) found all monocots eudicots, suggesting regulation critical function.The next sought determine controlled these elements.In addition ZmWUS1 duplication, experienced rearrangement leading 4 motifs ZmWUS1-B promoter.The edit obtained several alleles deletions different sizes.Alleles containing smaller deletions, 15-bp deletion Bif3-Δ15, phenotypically similar unedited gene-edited fewer copies

Язык: Английский

Процитировано

0
Evolutionary conservation of receptor compensation for stem cell homeostasis in Solanaceae plants DOI Creative Commons

Myeong-Gyun Seo,

Yoonseo Lim,

Anat Hendelman

и другие.

Horticulture Research, Год журнала: 2024, Номер 11(6)

Опубликована: Май 3, 2024

Abstract Stem cell homeostasis is pivotal for continuous and programmed formation of organs in plants. The precise control meristem proliferation mediated by the evolutionarily conserved signaling that encompasses complex interactions among multiple peptide ligands their receptor-like kinases. Here, we identified compensation mechanisms involving CLAVATA1 (CLV1) receptor its paralogs, BARELY ANY MERISTEMs (BAMs), stem two Solanaceae species, tomato groundcherry. Genetic analyses higher-order mutants deficient genes, generated via CRISPR-Cas9 genome editing, reveal SlBAM1 SlBAM2 compensate slclv1 mutations. Unlike compensatory responses between orthologous receptors observed Arabidopsis, mutations do not trigger transcriptional upregulation four SlBAM genes. within are also groundcherry, critical amino acid residues associated with physical interaction highly Our findings demonstrate evolutionary conservation both coding sequences kinases provides a strong buffering capacity during

Язык: Английский

Процитировано

0
Functions and Regulation of HAM Family Genes in Meristems During Gametophyte and Sporophyte Generations DOI Creative Commons
Yuan Geng,

Chong Xie,

Cankui Zhang

и другие.

Plant Cell & Environment, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 18, 2024

ABSTRACT A fascinating feature of land plants is their ability to continually initiate new tissues and organs throughout lifespan, driven by a pool pluripotent stem cells located in meristems. In seed plants, various types meristems are initiated maintained during the sporophyte generation, while gametophytes lack rely on for growth. contrast, seed‐free vascular such as ferns, develop both gametophyte generations, allowing independent growth generations. Recent findings have highlighted conserved lineage‐specific roles HAIRY MERISTEM (HAM) family GRAS‐domain transcriptional regulators plant lifecycle. Here, we review discuss how HAM genes maintain meristem indeterminacy sporophytes gametophytes, with focus studies performed two model species: flowering Arabidopsis thaliana fern Ceratopteris richardii . Additionally, summarize crucial tightly regulated functions microRNA171 (miR171)‐HAM regulatory modules, which define spatial patterns activities development across identities plants.

Язык: Английский

Процитировано

0