Arabidopsis CIRP1 E3 ligase modulates drought and oxidative stress tolerance and reactive oxygen species homeostasis by directly degrading catalases

Journal of Integrative Plant Biology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 28, 2025

ABSTRACT Reactive oxygen species (ROS) plays critical roles in modulating plant growth and stress response its homeostasis is fine tuned using multiple peroxidases. H 2 O , a major kind of ROS, removed rapidly directly three catalases, CAT1, CAT2, CAT3, Arabidopsis. Although the activity regulations catalases have been well studied, their degradation pathway less clear. Here, we report that CAT2 CAT3 protein abundance was partially controlled 26S proteasome. To further identify candidate proteins modulate stability performed yeast‐two‐hybrid screening recovered several clones encoding with RING vWA domains, CIRP1 ( C AT2 I nteracting R ING P rotein 1 ). Drought oxidative downregulated transcripts. harbored E3 ubiquitination accelerated by direct interaction ubiquitination. The cirp1 mutants exhibited stronger drought tolerance, which opposite to cat2 cat3 mutants. Genetic analysis revealed acts upstream negatively regulate tolerance. increased tolerance due enhanced catalase (CAT) activities alleviated ROS levels. Our data CIRP1–CAT2/CAT3 module vital role alleviating levels balancing responses

Язык: Английский

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A new scheme for boosting in situ Fenton-like reaction in plant pathogenic tissues for selective structural degradation of capsid proteins

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Фев. 8, 2025

Язык: Английский

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Expression of PSMD2 gene in hepatocellular carcinoma and its correlation with immune checkpoints and prognosis

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 24, 2025

Hepatocellular carcinoma (HCC) is a prevalent and fatal tumor globally, characterized by complex pathogenesis poor prognosis. Despite significant advancements in the application of immune checkpoint inhibitors (ICIs) for cancer treatment, efficacy immunotherapy HCC remains suboptimal. PSMD2, crucial regulator ubiquitin-proteasome system, has attracted increasing attention its involvement various cancers; however, functions mechanisms are still poorly understood. This study aims to investigate expression PSMD2 HCC, association with prognosis, interaction checkpoints, thus establishing foundation further exploration role evasion HCC. We analyzed levels adjacent normal tissues utilizing GEPIA TIMER databases. Cox regression analysis was performed using R software evaluate relationship between Furthermore, we assessed correlation cell infiltration, as well including PD1, PD-L1, CTLA-4, tools. Additionally, examined therapy response through Tumor Immune Dysfunction Exclusion (TIDE) analysis. Finally, constructed protein-protein (PPI) network STRING database Cytoscape software, followed Gene Set Enrichment Analysis (GSEA). significantly overexpressed closely correlated prognosis (HR = 1.61, P 2.0e-4). infiltration demonstrated that positively several genes, types. TIDE indicated elevated associated increased potential immunotherapy. GSEA enrichment revealed primarily enriched p53 signaling pathway, ubiquitin-mediated proteolysis other cancer-related pathways. The not only but may also play modulating immunity, thereby affecting patient responses Consequently, presents promising novel therapeutic target biomarker

Язык: Английский

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Neophytadiene, a Plant Specialized Metabolite, Mediates the Virus‐Vector‐Plant Tripartite Interactions

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Апрель 3, 2025

While interactions between viruses and their vectors, as well host plants, have been extensively studied, the genetic mechanisms underlying tripartite remain largely unknown. In this study, phenotypic assays are integrated with molecular biology functional genomic approaches to elucidate involving tomato chlorosis virus (ToCV), a major threat production worldwide, whitefly, Bemisia tabaci, an insect vector, plants. ToCV infection induces of chlorophyll degradation product that acts volatile attractant for whiteflies. Furthermore, suppression Lhca4, gene encoding subunit light-harvesting complex I in by P9 protein leads neophytadiene biosynthesis. Overexpression Lhca4 reduced infection. OBP2, odorant-binding from B. capable binding is identified. Suppression BtOBP2 impaired vector's subsequent preference ToCV-infected The results not only reveal underpinnings, including P9, plant whitefly BtOBP2, governing virus-vector-plant interactions, but also highlight neophytadiene, specialized metabolite mediator intricate multitrophic suggesting new avenues managing vectored insects.

Язык: Английский

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Molecular identification of sugarcane glyceraldehyde-3-phosphate dehydrogenases and their involvement in SCMV infection in sugarcane

Industrial Crops and Products, Год журнала: 2025, Номер 232, С. 121225 - 121225

Опубликована: Май 24, 2025

Язык: Английский

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Cucumber Green Mottle Mosaic Virus Coat Protein Hijacks Mitochondrial ATPδ to Promote Viral Infection

Molecular Plant Pathology, Год журнала: 2024, Номер 25(11)

Опубликована: Ноя. 1, 2024

ABSTRACT The production and scavenging of reactive oxygen species (ROS) are critical for plants to adapt biotic abiotic stresses. In this study, we investigated the interaction between coat protein (CP) cucumber green mottle mosaic virus (CGMMV) ATP synthase subunit δ (ATPδ) in mitochondria. Silencing ATPδ by tobacco rattle virus‐based virus‐induced gene silencing impeded CGMMV accumulation Nicotiana benthamiana leaves. Both overexpression transgenic transient expression promoted infection. Nitro blue tetrazolium (NBT) 3,3′‐diaminobenzidine (DAB) staining revealed that inhibited O 2 − but not H production. treatment CGMMV‐infected leaves with ROS inhibitor diphenylene iodonium (DPI) induced a burst Reverse transcription‐quantitative PCR superoxide dismutase (SOD) activity assays showed ATPδ, infection, CP specifically NbFeSOD3/4 SOD activity, We speculate interacts hijacks it, thereby enhancing quenching upregulating NbFeSOD and, turn, activity.

Язык: Английский

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Beyond movement: expanding functional landscape of luteovirus movement proteins

Trends in Plant Science, Год журнала: 2024, Номер unknown

Опубликована: Сен. 1, 2024

Язык: Английский

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A viral p3a protein targets and inhibits TaDOF transcription factors to promote the expression of susceptibility genes and facilitate viral infection

PLoS Pathogens, Год журнала: 2024, Номер 20(11), С. e1012680 - e1012680

Опубликована: Ноя. 7, 2024

The interactions among viruses and host plants are complex fascinating because these organisms interact with adapt to each other continuously. Many plant transcription factors play important roles in growth development the resistance viral infection. To facilitate infection of plants, some proteins typically target inhibit function factors. In this study, we found an interesting phenomenon wherein p3a protein barley yellow dwarf virus (BYDV) can zinc finger domain TaDOF factor wheat; promoter TaHSP70 gene; interacts through competitive binding, alleviating domain-mediated inhibition promoter, thereby promoting expression by BYDV. This study demonstrates that BYDV is immunosuppressive enriches our understanding pathogenesis

Язык: Английский

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A proposed newTombusviridaegenus featuring extremely long 5’ untranslated regions and a luteo/polerovirus-like gene block

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 24, 2024

Summary Tombusviridae is a large family of single-stranded, positive-sense RNA plant viruses with uncapped, non-polyadenylated genomes encoding 5-7 open reading frames (ORFs). Previously, we discovered, by high-throughput sequencing maize and teosinte RNA, novel genome virus call Maize-associated tombusvirus (MaTV). Here determined the precise termini MaTV using 5’ 3’ rapid amplification cDNA ends (RACE). In GenBank, discovered eleven other nearly complete viral MaTV-like organizations related RNA-dependent polymerase (RdRp) sequences. These came from diverse plant, fungal, invertebrate vertebrate organisms, some have been found in multiple organisms across globe. The available untranslated regions (UTRs) these are remarkably long: at least 438 to 727 nucleotides (nt), contrast those tombusvirids, which <150 nt. Moreover UTRs contain 6 12 AUG triplets that unlikely be start codons, because - possible exception there no or conserved ORFs UTRs. Such features suggest an internal ribosome entry site (IRES), but secondary structures. 50 nt upstream adjacent ORF1 codon, UTR was cytosine-rich guanosine-poor. As most ORF2 (RdRp gene) appears translated in-frame ribosomal readthrough stop codon. Indeed, all twelve identified structures known tombusviruses facilitate this readthrough. ORF5 predicted ORF3 (coat protein codon as genus Luteovirus . resulting domains highly divergent. ORF4 overlaps may initiate non-AUG We also obvious cap-independent translation elements, present tombusvirids. diverge sufficiently tombusvirids warrant classification new genus. Because they two leaky codons potential propose name Rimosavirus ( rimosa = Latin).

Язык: Английский

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A proposed new Tombusviridae genus featuring extremely long 5’ untranslated regions and a luteo/polerovirus-like gene block

Frontiers in Virology, Год журнала: 2024, Номер 4

Опубликована: Авг. 9, 2024

Tombusviridae is a large family of single-stranded, positive-sense RNA plant viruses with uncapped, non-polyadenylated genomes encoding 4–7 open reading frames (ORFs). Previously, we discovered, by high-throughput sequencing maize and teosinte RNA, novel genome virus call Maize-associated tombusvirus (MaTV). Here determined the precise termini MaTV using 5’ 3’ rapid amplification cDNA ends (RACE). In GenBank, discovered eleven other nearly complete viral MaTV-like organizations related RNA-dependent polymerase (RdRp) sequences. These came from diverse plant, fungal, invertebrate vertebrate organisms, some have been found in multiple organisms across globe. The available untranslated regions (UTRs) these are remarkably long: at least 438 to 727 nucleotides (nt), contrast those tombusvirids, which &lt;150 nt. Moreover UTRs contain 6 12 AUG triplets that unlikely be start codons, because - possible exception there no or conserved ORFs UTRs. Such features suggest an internal ribosome entry site (IRES), but only were 50 nt upstream adjacent ORF1 codon cytosine-rich guanosine-poor. ORF2 (RdRp gene) appears translated in-frame ribosomal readthrough stop codon. all twelve identified structures known tombusvirids facilitate this readthrough. ORF4 overlaps ORF3 (coat protein may initiate non-AUG ORF5 predicted proteins encoded 4 5 diverge highly each similarly organized luteo- poleroviruses. We also obvious cap-independent translation elements, present tombusvirids. sufficiently warrant classification new genus. Because they two leaky codons potential codon, propose name genus Rimosavirus ( rimosa = Latin).

Язык: Английский

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