Characterization of Interaction Between Low‐Density Lipoprotein (LDL) and Alkaloid N‐Methyl Cytisine: The Potential Protective Effect on Lipid Oxidation DOI

A. Bucci,

Ana Yanina Bustos,

María de los Angeles Frías

и другие.

ChemistrySelect, Год журнала: 2025, Номер 10(7)

Опубликована: Фев. 1, 2025

Abstract Elevated levels of low‐density lipoprotein (LDL) in the blood are one main risk factors for atherosclerosis and coronary heart disease. Oxidized LDL (ox‐LDL) plays an important role pathogenesis atherogenesis. The interaction with natural alkaloids may exert a protective effect on oxidation lipoproteins. In this study, binding N ‐methylcytisine (NMC) to is examined at molecular level, its investigated. was isolated by ultracentrifugation quantitatively characterized, Bradford technique, using SDS‐PAGE technique resolution 8% size measured dynamic light scattering (DLS). characteristic bands attributed functional groups lipoproteins fraction, were analyzed Fourier transform infrared spectroscopy (FTIR‐ATR). Using UV–visible, Raman, FTIR fluorescence spectroscopies, we found that interacts alkaloid NMC has against oxidation. Protein‐protein ligand‐protein docking methods used construct model complex between I domain apoprotein B100 NMC. Our results indicate occurs I‐domain apoB‐100 involves polar hydrophobic residues.

Язык: Английский

Characterization of Interaction Between Low‐Density Lipoprotein (LDL) and Alkaloid N‐Methyl Cytisine: The Potential Protective Effect on Lipid Oxidation DOI

A. Bucci,

Ana Yanina Bustos,

María de los Angeles Frías

и другие.

ChemistrySelect, Год журнала: 2025, Номер 10(7)

Опубликована: Фев. 1, 2025

Abstract Elevated levels of low‐density lipoprotein (LDL) in the blood are one main risk factors for atherosclerosis and coronary heart disease. Oxidized LDL (ox‐LDL) plays an important role pathogenesis atherogenesis. The interaction with natural alkaloids may exert a protective effect on oxidation lipoproteins. In this study, binding N ‐methylcytisine (NMC) to is examined at molecular level, its investigated. was isolated by ultracentrifugation quantitatively characterized, Bradford technique, using SDS‐PAGE technique resolution 8% size measured dynamic light scattering (DLS). characteristic bands attributed functional groups lipoproteins fraction, were analyzed Fourier transform infrared spectroscopy (FTIR‐ATR). Using UV–visible, Raman, FTIR fluorescence spectroscopies, we found that interacts alkaloid NMC has against oxidation. Protein‐protein ligand‐protein docking methods used construct model complex between I domain apoprotein B100 NMC. Our results indicate occurs I‐domain apoB‐100 involves polar hydrophobic residues.

Язык: Английский

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