Bioinspired Synthesis of Silver Nanoparticles Using L. Reuteri: Antibacterial Efficacy, Molecular Docking Insights, and Cytotoxicity Assessment

ChemistrySelect, Год журнала: 2025, Номер 10(2)

Опубликована: Янв. 1, 2025

Abstract This study reports the green synthesis of silver nanoparticles (AgNps) by using Lactobacillus reuteri . Characterized AgNps exhibited near‐spherical morphology with an average size 86.54 nm, as revealed SEM (scanning electron microscopy), TEM (transmission and AFM (atomic force microscopy) analyses. The displayed a slightly rough surface, some particles exhibiting surface protrusions sharp edges. zeta potential measurement −30.16 mV electrophoretic mobility −2.36 cm 2 /Vs that indicated presence negatively charged functional groups on suggesting stabilizing biomolecules prevent nanoparticle aggregation in solution. hydrodynamic was determined to be 140.3 XRD (X‐ray diffraction) analysis crystallinity 76.89% crystal 8.35 nm interplanar distance 0.233 nm. demonstrated potent antibacterial activity against Pseudomonas aeruginosa (92.9% killing efficiency) ATCC reference strain (76.8%). Molecular docking moderate interactions between optimized Ag 3 cluster key amino acid residues (Arg277, Gln279, Trp280, Asp273, Val276) P. protein These interactions, binding energies ranging from −1.89 −3.68 kcal/mol, suggested mechanism for observed activity. A charge transfer interaction involving contributing stabilization energy 3.66 identified factor Ag‐protein complex formation.

Язык: Английский

Bioactive silver nanoparticles derived from Carica papaya floral extract and its dual-functioning biomedical application

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 15, 2025

Replacing synthetic phytochemicals with natural plant extracts for metal nanoparticle synthesis enable cost-effective, large-scale production reduced environmental and health risks while enhancing biomedical efficacy. This study presents the green of silver nanoparticles (AgNPs) using a flavonol-enriched extract from male papaya flowers (KQE), an underutilized agricultural waste. Using 20% (v/v) KQE, highly stable, spherical KQ-AgNPs (12.3 ± 3.0 nm) were synthesized via in-situ generation free radicals, such as ortho-quinones, which Ag+ ions. exhibit superior antibacterial activity against both gram-positive gram-negative bacteria compared to chemically AgNPs (AgNPs-Chem) KQE alone. In vitro anticancer assays reveal enhanced cytotoxicity breast carcinoma cells (MCF-7) IC50 21.25 1.14 µg/mL, significantly lower than AgNPs-Chem (33.05 3.13 µg/mL), maintaining high biocompatibility normal (HEK-293) greater 169.96 2.3 µg/mL. highlights dual therapeutic potential KQ-AgNPs, emphasizing their efficacy exemplifying innovative waste-to-wealth approach.

Язык: Английский

Simple and hybrid materials for antimicrobial applications

Colloids and Surfaces B Biointerfaces, Год журнала: 2025, Номер 253, С. 114747 - 114747

Опубликована: Май 2, 2025

Язык: Английский