A multidimensional study for design of phytochemical profiling, antioxidant potential, and enzyme inhibition effects of ışgın (Rheum telianum) as an edible plant

Food Chemistry X, Год журнала: 2025, Номер 25, С. 102125 - 102125

Опубликована: Янв. 1, 2025

The present study reveals in vitro antioxidant properties and the phytochemical content of a novel Rheum species (Rheum telianum), which grows Southeastern Anatolia. To perform analysis, dried leaves seeds plants were ground extracted with ethanol to obtain plant secondary metabolites activity. Then, extracts subjected DPPH scavenging Cupric reducing (CUPRAC), Fe3+, Ferric power (FRAP). In addition capacity assays, quantitative phenolic, flavonoid, metabolite determined through spectrophotometric LC-MS/MS chromatographic methods. IC50 values showed that both R. telianum have high inhibitory over radicals 20.79 5.67 μg/mL, respectively. samples' dominant evaluated analysis results. inhibition effects on acetylcholinesterase butyrylcholinesterase, α-glycosidase human carbonic anhydrases II isoenzyme enzymes, associated some global diseases including Alzheimer's disease, type-2 diabetes mellitus glaucoma determined. conclusion, extracts' contents functional relationship plants' possible usage food, medicine, cosmetic industries was revealed.

Язык: Английский

Cardaria draba subspecies Shalepensis exerts in vitro and in silico inhibition of α-glucosidase, TRP1, and DLD-1 proliferation

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 26, 2025

In this study, in vitro enzyme activity assays were performed to investigate the inhibitory effects on α-glucosidase and tyrosinase-related protein 1, while silico molecular docking, dynamics, dynamics analyses provide information mechanisms. According obtained from approaches, inhibition properties are responsible for conformational changes structures, occupation of active site cleft by dominant compounds extract, as well long-term folding due departure usual motion. The IC50 values Cardaria draba (L.) DESV. subsp. Chalepensis extract 1 determined 1.89 ± 0.13 µg/ml 1.53 µg/ml, respectively. addition, value antiproliferative DLD-1 colon cancer cells was found be 6.9 µg/mL. Preclinical trials warranted validate extract's therapeutic potential. These findings suggest that exhibits properties, warranting further investigation its potential role interventions. Further research, particularly vivo studies, is required explore address DLD-1.

Язык: Английский

Phytochemical Analysis, Antioxidant, Anticholinergic, Antidiabetic, and Antiglaucoma Potentials of Sage (Salvia adiyamanensis)

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Апрель 10, 2025

In this study, the antioxidant effect of ethanolic extracts were prepared from aerial part Salvia adiyamanensis was investigated through five methods, including DPPH⋅, ABTS, and Fe³⁺, Cu²⁺, Fe³⁺-TPTZ reducing assays. The IC50 values extract determined as 35.40 ± 8.35 29.50 5.12 µg/mL for DPPH ABTS radicals scavenging enzyme inhibition property against α-glycosidase, acetylcholinesterase, butyrylcholinesterase, human carbonic anhydrase I, II, a result, IC₅₀ 30.78, 191.3, 8.02, 32.68 54.82 µg/mL, respectively. LC-MS/MS revealed that S. compromised characteristic phenolics rosmarinic acid, chlorogenic luteolin, quercetin, apigenin, caffeic acid Salvia genus. These findings strongly suggest has potential to be natural medicine treatment metabolic diseases such diabetes, Alzheimer’s disease, or glaucoma.

Язык: Английский