Polyamidoamine PAMAM G3.5-hematin nanozyme with enhanced peroxidase catalytic activities and biocompatibility for in situ hydrogelation DOI

Thanh Phu Nguyen,

Dinh Trung Nguyen,

Nguyen Huy Du

и другие.

Advances in Natural Sciences Nanoscience and Nanotechnology, Год журнала: 2025, Номер 16(2), С. 025015 - 025015

Опубликована: Апрель 24, 2025

Abstract Hematin has attracted great attention as an alternative catalyst to horseradish peroxidase (HRP) due its similar structure the iron-heme group in center. However, catalytic activities at neutral pH have been restricted poor solubility, which might be a drawback biomedical applications. Therefore, this study, we modified hematin with polyamidoamine G3.5 create novel HRP-mimicking biocatalyst (G3.5-He) enhanced oxidative activity under conditions, is ultimately used for catalyzing hydrogelation of phenol-conjugated gelatin. The chemical G3.5-He was analyzed by UV–vis, 1 H-NMR, and Fourier transform infrared spectroscopy, whereas size distribution nanoparticles were investigated varying conjugation degree transmission electron microscopy dynamic light scattering measurements. Meanwhile, biomimetic nanoenzyme carried out on catechol rutin oxidation assay, showed that possesses comparable more resistant against H 2 O than native HRP enzyme. In addition, effective biosensor quantify glucose level reaction ABTS. Interestingly, mild aqueous condition, rapidly catalyzed crosslinking gelatin-tyramine polymers situ formation hydrogel, resulting hydrogel excellent biocompatibility hemocompatibility. Taken together, suggest potential fabricate injectable hydrogels based phenol-rich various

Язык: Английский

Polymeric Nanosystems: A Breakthrough Approach to Treating Inflammation and Inflammation Related Diseases DOI Open Access
Maja D. Marković, Vesna V. Panić, Rada Pjanović

и другие.

Biopolymers, Год журнала: 2025, Номер 116(3)

Опубликована: Март 19, 2025

ABSTRACT Inflammation processes can cause mild to severe damage in the human body and lead a large number of inflammation‐related diseases (IRD) such as cancer, neural, vascular, pulmonary diseases. Limitations anti‐inflammatory drugs (AID) application are reflected high therapeutic doses, toxicity, low bioavailability solubility, side effects, etc. Polymeric nanosystems (PS) have been recognized safe effective technology that is able overcome these limitations by AID encapsulation answer specific demands IRD treatment. PS attracting great attention due their versatility, biocompatibility, fine‐tuned properties, functionality, ability for precise delivery targeted sites body. This article offers an overview three classes polymeric nanosystems: a) dendrimers, b) micelles nanoparticles, c) filomicelles, well preparation, In future, formulations clinical practice will certainly increase.

Язык: Английский

Процитировано

0
Polyamidoamine PAMAM G3.5-hematin nanozyme with enhanced peroxidase catalytic activities and biocompatibility for in situ hydrogelation DOI

Thanh Phu Nguyen,

Dinh Trung Nguyen,

Nguyen Huy Du

и другие.

Advances in Natural Sciences Nanoscience and Nanotechnology, Год журнала: 2025, Номер 16(2), С. 025015 - 025015

Опубликована: Апрель 24, 2025

Abstract Hematin has attracted great attention as an alternative catalyst to horseradish peroxidase (HRP) due its similar structure the iron-heme group in center. However, catalytic activities at neutral pH have been restricted poor solubility, which might be a drawback biomedical applications. Therefore, this study, we modified hematin with polyamidoamine G3.5 create novel HRP-mimicking biocatalyst (G3.5-He) enhanced oxidative activity under conditions, is ultimately used for catalyzing hydrogelation of phenol-conjugated gelatin. The chemical G3.5-He was analyzed by UV–vis, 1 H-NMR, and Fourier transform infrared spectroscopy, whereas size distribution nanoparticles were investigated varying conjugation degree transmission electron microscopy dynamic light scattering measurements. Meanwhile, biomimetic nanoenzyme carried out on catechol rutin oxidation assay, showed that possesses comparable more resistant against H 2 O than native HRP enzyme. In addition, effective biosensor quantify glucose level reaction ABTS. Interestingly, mild aqueous condition, rapidly catalyzed crosslinking gelatin-tyramine polymers situ formation hydrogel, resulting hydrogel excellent biocompatibility hemocompatibility. Taken together, suggest potential fabricate injectable hydrogels based phenol-rich various

Язык: Английский

Процитировано

0