Hemoglobin‐Mediated Dual‐Crosslinked Silk Fibroin With Enhanced Elasticity, Rigidity, Toughness, and Shapeability for Tracheal Tissue Application DOI

Zhiyi Guo,

Xiang Fei, Bin Zhou

и другие.

Advanced Functional Materials, Год журнала: 2025, Номер unknown

Опубликована: Апрель 14, 2025

Abstract The study presents an elastic, rigid, and shapeable silk fibroin (SF)‐based scaffold with a dual‐crosslinked network for tracheal tissue engineering. Hemoglobin (Hb), biocompatible cost‐effective natural protein, is introduced as both catalyst crosslinker. in situ chemical crosslinking occurred via dityrosine reaction the presence of hydrogen peroxide. Subsequently, freeze‐drying induced formation β‐sheet crystals, further physically SF chains, resulting 3D porous Hb‐SF scaffold. Additionally, α‐helix subunits Hb acted “molecular springs”, enhancing scaffold's mechanical strength, elasticity, toughness. biomechanical properties are tunable by adjusting content, enabling precise shape fidelity, self‐recovery, durability. Notably, exhibited high fatigue resistance, enduring multiple compressive cycles without damage. In vitro tests confirmed its cytocompatibility potential cartilage regeneration. Furthermore, vivo implantation chondrocyte‐seeded scaffolds resulted biomimetic trachea structural closely resembling those native trachea. This Hb‐mediated, demonstrates enhanced rigidity, toughness, shapeability, presenting viable option regeneration applications.

Язык: Английский

Dynamic Col-HZ Hydrogel with Efficient Delivery of Bioactivator Promotes ECM Deposition and Cartilage Formation DOI Creative Commons

Honglei Wang,

Xu Wu, Lili Chen

и другие.

Materials Today Bio, Год журнала: 2025, Номер 31, С. 101623 - 101623

Опубликована: Фев. 28, 2025

Efforts in cartilage tissue engineering to repair injuries have seen limited success, primarily due the inability of scaffold materials establish a microenvironment conducive extracellular matrix (ECM) deposition by chondrocytes. Hydrogels, which mimic human tissue, are commonly employed as materials; however, their constrained network structure and low bioactivity impede chondrocyte ECM deposition, complicating repair. In this study, we developed dynamic Col-HZ hydrogels featuring adaptive networks forming hydrazone (HZ) bonds between bioactive natural collagen synthetic polyethylene glycol (PEG). contrast static that rely on covalent bonds, facilitate migration deposition. Additionally, aldehyde groups hydrogel can engage Schiff base bonding with amine groups. Leveraging non-covalent interaction, incorporated bioactivator TD-198946, known enhance synthesis, into hydrogel. This significantly boosted reduced inflammation. Transcriptomic sequencing bioinformatics analyses indicate both binding TD-198946 promote through modulation Wnt/β-catenin signaling pathway. Consequently, hydrogel, combination creates an improved supports facilitates formation.

Язык: Английский

Процитировано

0
Hemoglobin‐Mediated Dual‐Crosslinked Silk Fibroin With Enhanced Elasticity, Rigidity, Toughness, and Shapeability for Tracheal Tissue Application DOI

Zhiyi Guo,

Xiang Fei, Bin Zhou

и другие.

Advanced Functional Materials, Год журнала: 2025, Номер unknown

Опубликована: Апрель 14, 2025

Abstract The study presents an elastic, rigid, and shapeable silk fibroin (SF)‐based scaffold with a dual‐crosslinked network for tracheal tissue engineering. Hemoglobin (Hb), biocompatible cost‐effective natural protein, is introduced as both catalyst crosslinker. in situ chemical crosslinking occurred via dityrosine reaction the presence of hydrogen peroxide. Subsequently, freeze‐drying induced formation β‐sheet crystals, further physically SF chains, resulting 3D porous Hb‐SF scaffold. Additionally, α‐helix subunits Hb acted “molecular springs”, enhancing scaffold's mechanical strength, elasticity, toughness. biomechanical properties are tunable by adjusting content, enabling precise shape fidelity, self‐recovery, durability. Notably, exhibited high fatigue resistance, enduring multiple compressive cycles without damage. In vitro tests confirmed its cytocompatibility potential cartilage regeneration. Furthermore, vivo implantation chondrocyte‐seeded scaffolds resulted biomimetic trachea structural closely resembling those native trachea. This Hb‐mediated, demonstrates enhanced rigidity, toughness, shapeability, presenting viable option regeneration applications.

Язык: Английский

Процитировано

0