Controlling DNA Fragments Translocation across Nanopores with the Synergic Use of Site-Directed Mutagenesis, pH-Dependent Charge Tuning, and Electroosmotic Flow
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(30), С. 40100 - 40110
Опубликована: Июль 22, 2024
Biological
and
solid-state
nanopores
are
at
the
core
of
transformative
techniques
nanodevices,
democratizing
examination
matter
biochemical
reactions
single-molecule
level,
with
low
cost,
portability,
simplicity
in
operation.
One
crucial
hurdles
such
endeavors
is
fast
analyte
translocation,
which
limits
characterization,
a
rich
number
strategies
have
been
explored
over
years
to
overcome
this.
Here,
by
site-directed
mutagenesis
on
α-hemolysin
protein
nanopore
(α-HL),
sought
replace
selected
amino
acids
glycine,
electrostatic
binding
sites
were
induced
nanopore's
vestibule
constriction
region
achieved
most
favorable
case
20-fold
increase
translocation
time
short
single-stranded
DNA
(ssDNA)
neutral
pH,
respect
wild-type
(WT)
nanopore.
We
demonstrated
an
efficient
tool
controlling
ssDNA
time,
via
interplay
between
nanopore-ssDNA
surface
interactions
electroosmotic
flow,
all
mediated
pH-dependent
ionization
lining
pathway.
Our
data
also
reveal
nonmonotonic,
pH-induced
alteration
average
time.
Unlike
mildly
acidic
conditions
(pH
∼
4.7),
pH
2.8
maintained
symmetrically
or
asymmetrically
across
WT
α-HL,
we
evidenced
manifestation
dominant
determining
speeding
up
counteracting
ssDNA-nanopore
attractive
interactions.
envision
potential
applications
presented
approach
enabling
easy-to-use,
real-time
detection
sequences,
without
need
for
complex
modifications
mitigate
sequences.
Язык: Английский
Nanopore‐based detection of periodontitis biomarker miR31 in saliva samples
Electrophoresis,
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 21, 2024
MicroRNAs
(miRNAs)
play
important
roles
in
posttranscriptional
gene
regulation.
Aberrations
the
miRNA
levels
have
been
cause
behind
various
diseases,
including
periodontitis.
Therefore,
sensitive,
specific,
and
accurate
detection
of
disease-associated
miRNAs
is
vital
to
early
diagnosis
can
facilitate
inhibitor
screening
drug
design.
In
this
study,
we
developed
a
label-free,
real-time
sensing
method
for
miR31,
which
has
frequently
linked
periodontitis,
using
an
engineered
protein
nanopore
presence
complementary
ssDNA
as
molecular
probe.
Our
rapid
highly
sensitive
with
nanomolar
concentration
miR31
that
could
be
determined
minutes.
Furthermore,
our
sensor
showed
high
selectivity
toward
target
sequence
even
interfering
nucleic
acids.
addition,
artificial
saliva
human
samples
were
successfully
analyzed.
platform
used
detect
other
offers
potential
application
clinical
disease
biomarkers.
Язык: Английский
Molecular sandwich-based DNAzyme catalytic reaction towards transducing efficient nanopore electrical detection for antigen proteins
Faraday Discussions,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
A
molecular
sandwich-based
DNAzyme
catalytic
reaction
is
capable
of
transducing
detectable
nucleic
acids
as
a
substitute
for
difficult
to
yield
protein
detection
in
complicated
biological
matrices,
nanopore.
Язык: Английский
Protein nanopore-based sensors for public health analyte detection
Journal of Materials Chemistry B,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
High-throughput
and
label-free
protein
nanopore-based
sensors
with
engineering
strategies
are
extensively
used
to
identify
public
health
hazardous
substances
single
channel
recording.
Язык: Английский
Peptides in the detection of metal ions
Analytical Methods,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
By
means
of
their
specific
interactions
with
different
metal
ions,
naturally
occurring
proteins
control
structures
and
functions
many
biological
processes
in
organisms.
In
view
natural
metallopeptides,
scientists
have
proposed
artificial
peptides
which
coordinate
ions
through
functional
groups
either
for
introducing
a
special
reactivity
or
constructing
various
sensors.
However,
the
design
new
peptide
ligands
requires
deep
understanding
structures,
assembly
properties,
dynamic
behaviors
such
peptides.
This
review
briefly
describes
detection
strategies
Язык: Английский